Rapid profiling and pharmacokinetic studies of major compounds in crude extract from Polygonum multiflorum by UHPLC-Q-TOF-MS and UPLC–MS/MS

Wang, Linlin; Sang, Mangmang; Liu, Erwei; Banahene, Prince Osei; Zhang, Yi; Tao, Wang; Han, Lifeng; Gao, Xiumei

doi:10.1016/j.jpba.2017.03.016

Cited by 65 publications

(67 citation statements)

References 42 publications

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“…A simple, sensitive, and reliable UPLC-MS/MS method for the determination of the glycosides and aglycones of anthraquinones and 2,3,5,4′-tetrahydroxystilbene-2-O-β-Dglucoside in rat plasma was developed. is method is faster and more high-throughput with analytical time shortening from 18 min to 9 min while the number of simultaneous determined analytes increasing from 7 to 13 comparing with the previous reported method [38]. e method was acceptably validated and applied to a pharmacokinetic study of the constituents after oral administration of RPM extract in rats.…”

Section: Discussionmentioning

confidence: 87%

“…e ultraperformance liquid chromatography combined with triple quadrupole tandem mass spectrometry (UPLC-MS/MS), detection using different multiple reaction monitor (MRM) channels at the same time, is a powerful technique used for simultaneous quantification of multiple components in complex matrix due to its very high sensitivity and selectivity. Several LC-MS/MS analytical methods determining multicomponents of RPM and the application of pharmacokinetics-related investigations were reported [31,33,37,38]. However, at most 7 analytes of RPM were simultaneous determined according to the reports [38].…”

Section: Introductionmentioning

confidence: 99%

“…Several LC-MS/MS analytical methods determining multicomponents of RPM and the application of pharmacokinetics-related investigations were reported [31,33,37,38]. However, at most 7 analytes of RPM were simultaneous determined according to the reports [38]. erefore, the aim of this study was to develop and validate a method for simultaneous measurement of 13 components (chrysophanol, emodin, aloe-emodin, rhein, physcion, questin, citreorosein, questinol, TSG, torachrysone-8-O-glucoside (TG), chrysophanol-8-O-β-D-glucoside (CG), emodin-8-O-β-D-glucoside (EG), and physcion-8-O-β-D-glucoside (PG)) in rat plasma using an UPLC-MS/MS and investigate their pharmacokinetics after rats administration of RPM extract.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study

Cheng

Yang

et al. 2020

International Journal of Analytical Chemistry

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Radix Polygoni Multiflori (RPM) has been widely used to treat various diseases in Asian countries for many centuries. Although, stilbenes and anthraquinones, two major components of RPM, show various bioactive effects, it has been speculated that the idiosyncratic hepatotoxicity induced by RPM may be related to these constituents. However, information on the pharmacokinetics of stilbenes and anthraquinones at a subtoxic dose of RPM is limited. A simple and sensitive UPLC-MS/MS bioanalytical method for the simultaneous determination of 13 ingredients of RPM, including chrysophanol, emodin, aloe-emodin, rhein, physcion, questin, citreorosein, questinol, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside, torachrysone-8-O-glucoside, chrysophanol-8-O-β-D-glucoside, emodin-8-O-β-D-glucoside, and physcion-8-O-β-D-glucoside, in rat plasma was established. Acetonitrile was employed to precipitate the plasma with appropriate sensitivity and acceptable matrix effects. Chromatographic separation was performed using a waters HSS C18 column with a gradient elution using water and acetonitrile both containing 0.025% formic acid within a run time of 9 min. e constituents were detected in negative ionization mode using multiple reaction monitoring. e method was fully validated in terms of selectivity, linearity, accuracy, precision, recovery, matrix effects, and stability. e lower limit of quantitation of the analytes was 0.1-1 ng/mL. e intrabatch and interbatch accuracies were 87.1-109%, and the precision was within the acceptable limits. e method was applied to a pharmacokinetic study after oral administration of RPM extract to rats at a subtoxic dose of 36 g/kg.

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Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study

Cheng

Yang

et al. 2020

International Journal of Analytical Chemistry

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“…Ten compounds were unambiguously identified as chlorogenic acid, ferulic acid, nodakenin, bergapten, 3,4‐dicaffeoylquinic acid, 3,5‐dicaffeoylquinic acid, 4,5‐dicaffeoylquinic acid, imperatorin, notopterol and isoimperatorin (peaks 6, 16, 17, 18, 21, 22, 34, 38, 42 and 44, respectively) based on the retention time and mass spectra of these standards. The identification of other compounds was deduced by reference to previously published data, performing accurate m / z value analysis of the TOF‐MS data, and with QTOF‐MS/MS fragment analysis. All detected compounds are shown in Table .…”

Section: Resultsmentioning

confidence: 99%

Rapid discrimination of Notopterygium incisum and Notopterygium franchetii based on characteristic compound profiles detected by UHPLC‐QTOF‐MS/MS coupled with multivariate analysis

Liu

et al. 2020

Phytochemical Analysis

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Introduction The herbs Notopterygium incisum (NI) and N. franchetii (NF) are referred to as “Qianghuo” in the Chinese Pharmacopeia and are popular for treatment of certain conditions, including headaches, rheumatoid arthritis and the common cold. Recently, several adulterations of NI and NF have been found in the Chinese herbal market. Objective The aim of this study was to rapidly identify the unique characteristic compounds of NI and NF, to discriminate Qianghuo from its adulterations. Methodology Twenty‐four batches of NI and NF samples with different origins were collected and extracted with methanol. The extracts were analysed using ultrahigh‐performance liquid chromatography‐quadrupole time‐of‐flight tandem mass spectrometry (UHPLC‐QTOF‐MS/MS). Principal component analysis (PCA) and orthogonal partial squared discriminant analysis (OPLS‐DA) were then used to distinguish between NI and NF and to identify their potential characteristic markers. Results Fifty compounds were identified or tentatively characterised according to the retention time, m/z value and MS/MS fragment analysis. Six compounds were selected as potential markers of NI and NF by PCA and OPLS‐DA. They were successfully applied to authenticate 17 kinds of Chinese patent medicines containing Qianghuo. The markers could not be detected in three of the Chinese patent medicines, indicating that they were counterfeit products. Conclusion The UHPLC‐QTOF‐MS/MS coupled with the multivariate analysis method could discriminate NI and NF from their adulterations. Moreover, the data clearly demonstrated significant differences in the chemical compositions of NI and NF. Further research is needed to examine the relationship between therapeutic efficacy and the chemical constituents of NI and NF.

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“…A total of 87 compounds were definitely or tentatively identified in RPMR and PPMR by chemical standards and/or the established in-house database (Table 1; Qiu et al, 2013; Lin et al, 2015a; Wang et al, 2017). The representative LC-MS base peak chromatograms (BPCs) for RPMR and PPMR are shown in Figure 1.…”

Section: Resultsmentioning

confidence: 99%

Integrating Targeted and Untargeted Metabolomics to Investigate the Processing Chemistry of Polygoni Multiflori Radix

Liang

Zhou

et al. 2018

Front. Pharmacol.

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Polygoni Multiflori Radix (PMR, Heshouwu in Chinese), derived from the tuberous roots of Polygonum multiflorum Thunb., is a widely-used Chinese medicinal material. For traditional clinical use, raw PMR (RPMR) is processed by nine cycles of steaming and drying to generate processed PMR (PPMR); RPMR and PPMR have distinct medicinal purposes based on the theory of traditional Chinese medicine. While PMR has been processed for hundreds of years, including the present, the chemistry of that processing has not been well studied. In this study, targeted and untargeted metabolomics analyses using ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) and ultra-performance liquid chromatography-quadrupole/triple quadrupole mass spectrometry (UPLC-QqQ-MS/MS) were integrated to investigate the processing chemistry of PMR. The results demonstrate that processing by nine cycles of steaming and drying qualitatively and quantitatively alters the chemical profile of PMR. Several mechanisms, namely hydrolysis, dehydration, isomerization, and Maillard reaction appear to be involved in the chemical transformation that occurs. The qualitative and quantitative data further suggest that nine cycles might be necessary for the preparation of PPMR, as PPMR that has been processed nine times shows significant differences in its chemical profile.

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Rapid profiling and pharmacokinetic studies of major compounds in crude extract from Polygonum multiflorum by UHPLC-Q-TOF-MS and UPLC–MS/MS

Cited by 65 publications

References 42 publications

Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study

Simultaneous Determination of 13 Constituents of Radix Polygoni Multiflori in Rat Plasma and Its Application in a Pharmacokinetic Study

Rapid discrimination of Notopterygium incisum and Notopterygium franchetii based on characteristic compound profiles detected by UHPLC‐QTOF‐MS/MS coupled with multivariate analysis

Integrating Targeted and Untargeted Metabolomics to Investigate the Processing Chemistry of Polygoni Multiflori Radix

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