Rapid profiling of cytokinins using supercritical fluid chromatography coupled with tandem mass spectrometry

Petřík, Ivan; Pěnčík, Aleš; Stýskala, Jakub; Tranová, Lenka; Amakorová, Petra; Strnad, Miroslav; Novák, Ondřej

doi:10.1016/j.aca.2023.342010

Cited by 3 publications

(1 citation statement)

References 45 publications

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“…Improvements in high-pressure liquid chromatography (HPLC) have been made with the introduction of ultra-high-pressure liquid chromatography (UHPLC), supercritical fluid chromatography (SFC), and ultra-high-pressure supercritical fluid chromatography (UHPSFC), which reduce the amount of solvents necessary in analyses while improving resolution [48]. When UHPSFC-tandem mass spectroscopy is employed, the determination of plant hormones (cytokinins) can be analyzed in 9 min at detection limits close to 0.03 fmol [49]. ACS has introduced the analytical method greenness score (AMGS) calculator developed by Hicks et al [48] that ranks chromatography methods according to instrument energy, solvent energy, and solvent EHS scores [10].…”

Section: Chromatography Solventsmentioning

confidence: 99%

Solvent Replacement Strategies for Processing Pharmaceuticals and Bio-Related Compounds—A Review

Lee,

Chong,

Ota

et al. 2024

Liquids

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An overview of solvent replacement strategies shows that there is great progress in green chemistry for replacing hazardous di-polar aprotic solvents, such as N,N-dimethylformamide (DMF), 1-methyl-2-pyrrolidinone (NMP), and 1,4-dioxane (DI), used in processing active industrial ingredients (APIs). In synthetic chemistry, alcohols, carbonates, ethers, eucalyptol, glycols, furans, ketones, cycloalkanones, lactones, pyrrolidinone or solvent mixtures, 2-methyl tetrahydrofuran in methanol, HCl in cyclopentyl methyl ether, or trifluoroacetic acid in propylene carbonate or surfactant water (no organic solvents) are suggested replacement solvents. For the replacement of dichloromethane (DCM) used in chromatography, ethyl acetate ethanol or 2-propanol in heptanes, with or without acetic acid or ammonium hydroxide additives, are suggested, along with methanol acetic acid in ethyl acetate or methyl tert-butyl ether, ethyl acetate in ethanol in cyclohexane, CO2-ethyl acetate, CO2-methanol, CO2-acetone, and CO2-isopropanol. Supercritical CO2 (scCO2) can be used to replace many organic solvents used in processing materials from natural sources. Vegetable, drupe, legume, and seed oils used as co-extractants (mixed with substrate before extraction) can be used to replace the typical organic co-solvents (ethanol, acetone) used in scCO2 extraction. Mixed solvents consisting of a hydrogen bond donor (HBD) solvent and a hydrogen bond acceptor (HBA) are not addressed in GSK or CHEM21 solvent replacement guides. Published data for 100 water-soluble and water-insoluble APIs in mono-solvents show polarity ranges appropriate for the processing of APIs with mixed solvents. When water is used, possible HBA candidate solvents are acetone, acetic acid, acetonitrile, ethanol, methanol, 2-methyl tetrahydrofuran, 2,2,5,5-tetramethyloxolane, dimethylisosorbide, Cyrene, Cygnet 0.0, or diformylxylose. When alcohol is used, possible HBA candidates are cyclopentanone, esters, lactone, eucalytol, MeSesamol, or diformylxylose. HBA—HBA mixed solvents, such as Cyrene—Cygnet 0.0, could provide interesting new combinations. Solubility parameters, Reichardt polarity, Kamlet—Taft parameters, and linear solvation energy relationships provide practical ways for identifying mixed solvents applicable to API systems.

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Section: Chromatography Solventsmentioning

confidence: 99%

Solvent Replacement Strategies for Processing Pharmaceuticals and Bio-Related Compounds—A Review

Lee,

Chong,

Ota

et al. 2024

Liquids

View full text Add to dashboard Cite

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Rapid analysis of six cytokinins in tomato plants using phenylboronic acid and ionic liquid functionalized monolithic capillary column combined with UHPLC-MS/MS

Deng,

Yang,

et al. 2025

Journal of Chromatography A

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Spatio-temporal plant hormonomics: from tissue to subcellular resolution

Petřík,

Hladík,

Zhang

et al. 2024

Journal of Experimental Botany

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Due to technological advances in mass spectrometry, significant progress has been achieved recently in plant hormone research. Nowadays, plant hormonomics is well established as a fully integrated scientific field focused on the analysis of phytohormones, mainly on their isolation, identification and spatiotemporal quantification in plants. This review represents a comprehensive meta-study of the advances in the phytohormone analysis by mass spectrometry over the past decade. To address current trends and future perspectives, Web of Science data were systematically collected and key features such as mass spectrometry-based analyses were evaluated using multivariate data analysis methods. Our findings showed that plant hormonomics is currently divided into targeted and untargeted approaches. Both aim to miniaturize the sample, allowing high-resolution quantification to be covered in plant organs as well as subcellular compartments. Therefore, we can study plant hormone biosynthesis, metabolism and signalling at a spatio-temporal resolution. Moreover, this trend has recently been accelerated by technological advances such as fluorescence-activated cell sorting or mass spectrometry imaging.

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Rapid profiling of cytokinins using supercritical fluid chromatography coupled with tandem mass spectrometry

Cited by 3 publications

References 45 publications

Solvent Replacement Strategies for Processing Pharmaceuticals and Bio-Related Compounds—A Review

Solvent Replacement Strategies for Processing Pharmaceuticals and Bio-Related Compounds—A Review

Rapid analysis of six cytokinins in tomato plants using phenylboronic acid and ionic liquid functionalized monolithic capillary column combined with UHPLC-MS/MS

Spatio-temporal plant hormonomics: from tissue to subcellular resolution

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