Rapid Quantification and Validation of Biomarker Scopoletin in Paederia foetida by qNMR and UV–Vis for Herbal Preparation

Tan, Dai Chuan; Quek, Alexandra; Kassim, Nur Kartinee; Ismail, Intan Safinar; Lee, Joanna Jinling

doi:10.3390/molecules25215162

Cited by 14 publications

(10 citation statements)

References 27 publications

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“…Peak 5 and 6 exhibited closely related chromatographic and spectral features, which were in accordance with that of dicaffeoylquinic acid, and the two compounds were thus assigned to be isomeric compounds thereof. Peak 3 gave an UV absorbance spectrum similar to that of a 6,7-di-oxy-coumarin [ 41 ]. The base peak of the ESI+ spectrum was found to be the pseudomolecular ion at m / z 193 confirmed by the presence of [M+Na+H] + at m / z 215 and [M-H] − at m / z 191.…”

Section: Resultsmentioning

confidence: 99%

Chemical Profiling and Biological Activity of Extracts from Nine Norwegian Medicinal and Aromatic Plants

Johny

Thomsen²,

Karlsen

et al. 2022

Molecules

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There is an increased interest in identifying beneficial compounds of plant origin that can be added to animal diets to improve animal performance and have a health-promoting effect. In the present study, nine herb species of the Norwegian wild flora or which can be cultivated in Norway were selected for phytogenic evaluation (hops, maral root, mint, oregano, purslane, rosemary, roseroot, sweet wormwood, yarrow). Dried herbs were sequentially extracted with dichloromethane (DCM), ethanol (EtOH) and finally water (H2O) by ultrasound-assisted extraction (UAE). The UAE protocol was found to be more rational than conventional Soxhlet with respect to DCM extraction. Total extraction yield was found to be highest for oregano (Origanum vulgare) with 34.4 g 100−1 g dry matter (DM). H2O-extracts gave the highest yields of the three solvents, with up to 25 g 100−1 g DM for purslane (Portulaca oleracea ssp. sativa) and mint (Mentha piperita). EtOH- and H2O-extracts were the most efficient extracts with respect to free radical scavenging capacity (ABTS (=2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), and oregano, mint, hops (Humulus lupulus) and maral root-leaves (Leuzea carthamoides) were found to be the most efficient antioxidant sources. Hops (EtOH-extract) contained α- and β-acids, xanthohumols, chlorogenic acid and the hitherto unreported 3-O-glucosides of kaempferol and quercetin. Maral root-leaves contained among other compounds hexosides of the 6-hydroxy- and 6-methoxy-kaempferol and -quercetin, whereas roseroot (Rosea rhodiola) revealed contents of rosavin, rhodiosin and rhodionin. Sweet wormwood (Artemisia annua) contained chlorogenic acid and several derivatives thereof, scopoletin and poly-methylated flavones (eupatin, casticin, chrysoplenetin). Antimicrobial potential of different plant extracts was demonstrated against Gram-positive and Gram-negative bacteria using the indicator organisms Staphylococcus aureus, and Escherichia coli, and the Atlantic salmon bacterial pathogens Moritella viscosa, Tenacibaculum finnmarkense and Aliivibrio wodanis. DCM extracts possessed the highest activities. Data demonstrate the potential ability of herb extracts as natural antimicrobials. However, future safety studies should be performed to elucidate any compromising effect on fish health.

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Section: Resultsmentioning

confidence: 99%

Chemical Profiling and Biological Activity of Extracts from Nine Norwegian Medicinal and Aromatic Plants

Johny

Thomsen²,

Karlsen

et al. 2022

Molecules

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“…10 Moreover, in different ingredients of churna and in composite churna a rapid, sensitive UV spectroscopic method was developed by using gallic acid as marker at 271nm and validated by different parameters as mentioned in ICH. 11 Furthermore, the other literature review revealed that there are various methods available for the detection and quantification of eugenol alone or along with other constituents using UV-spectroscopy, 12 High-performance liquid chromatography 13 and LC-MS. 14 Likewise, scopoletin was estimated by UV-spectroscopy, 15 HPTLC 16 and High-performance liquid chromatography. 17 Hence, from the literature review it is clear that there is no method reported in pharmacopoeias for simultaneous analysis of eugenol and scopoletin by UV spectroscopy in Avipattikar churna.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous Estimation of Eugenol and Scopoletin by UV-Spectroscopic Method Using in-house Avipattikar churna

Shah¹,

Pundarikakshudu²,

Patel³

et al. 2023

J Young Pharm.

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Objectives: In this study, a new brisk, efficient and precise method of first derivative UV spectrophotometric was developed and validated for the estimation of eugenol and scopoletin simultaneously from the in-house Avipattikar churna. Materials and Methods: The method involved estimation of eugenol and scopoletin by simultaneous equation of first derivative spectra at 296 nm and 278.66 nm respectively in 0.1 N HCl. As per the Beer-Lambert law, proportional absorbance (linearity) was recorded in the concentration limit of 20-100μg/mL for both eugenol and scopoletin. Results: The method validation was done in reference to ICH (International council for harmonisation of technical requirements for pharmaceuticals for human use) norms comprising of accuracy, linearity, precision, the limit of detection, and quantification of standard from churna. This method was found to be precise as % relative standard deviation was less than 2%. The mean recoveries of triplicate samples were found in the range of 98.52 -101.30% and 100.51 -100.56 % for eugenol and scopoletin respectively. The limit of detection and limit of quantification were found to be 2.62μg/mL and 7.96μg/mL for eugenol respectively. The limit of detection and limit of quantification were found to be 1.54μg/mL 4.66μg/mL for scopoletin respectively. Conclusion: The developed estimation method was found to be specific, rapid, precise, and accurate for the analysis of eugenol and scopoletin in the Ayurvedic churna and in formulation without showing any interference.

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“…Substep seven: The yellow color was observed using the detector on exposure LED lamps as the modified Indonesia Patent Registered P00202107382 on September 9, 2021. The limit detection of RTD was evaluated based on the yellow color formation of the samples at the lowest concentrations of serial tetracycline dilutions [ 28 , 29 ]. Criteria of the color yellow and hypertext markup language code are as follows as presented in Figure-1 [ 30 - 32 ].…”

Section: Methodsmentioning

confidence: 99%

Designing prototype rapid test device at qualitative performance to detect residue of tetracycline in chicken carcass

2022

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Background and Aim: Human health problems due as a microbial resistance or tumors and cancers because consumption of the carcasses containing residues of tetracycline are main global problems in the context of fight against antimicrobial resistance phenomena. Explanation of the sustainable development goals, particularly point 3, is well recognized that all animal products for human consumption must be safe to live a healthy life. This study aimed to design a prototype of rapid test devices (RTD) based on principles of precipitate to obtain a specific color change after the process of reactions as an indicator to determine tetracycline residues in the carcass. Materials and Methods: Five samples of tetracycline-containing poultry carcasses using artificial add the tetracycline at pharmaceutics grade were examined using a prototype of a strong reaction solution for tetracycline fixation based on the concept bonded by ion Fe(III) at atom O in position atom C-1 at the ring of tetracycline and ion N+ as the functional branch of tetracycline. RTD detection was evaluated using a yellow color presentation and an absorbance spectrometric technique at a wavelength of 273 nm. Results: The following chemicals were used to create the best-fixed tetracycline residue: HCl and H2SO4 dissolved in H2O, chromatographic grade of 0.1 N and 0.5 N of HNO3, and 1% Fe (III) Cl. The RTD had a higher limit of detection (LOD) than the ultraviolet-visible spectrophotometer. Conclusion: The results of this study revealed that RTD, as constructed in this study, can be used to detect residue at LOD 44.764 μg/mL during 120 min of exposure through a light-emitting diode at 980 nm wavelength (p<0.05). The necessity for using RTD was because of the apparent limitations of conventional devices.

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Rapid Quantification and Validation of Biomarker Scopoletin in Paederia foetida by qNMR and UV–Vis for Herbal Preparation

Cited by 14 publications

References 27 publications

Chemical Profiling and Biological Activity of Extracts from Nine Norwegian Medicinal and Aromatic Plants

Chemical Profiling and Biological Activity of Extracts from Nine Norwegian Medicinal and Aromatic Plants

Simultaneous Estimation of Eugenol and Scopoletin by UV-Spectroscopic Method Using in-house Avipattikar churna

Designing prototype rapid test device at qualitative performance to detect residue of tetracycline in chicken carcass

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