Rapid Quantitative Method for Salmonella Detection in Polluted Waters

Abstract

A procedure has been developed for the enumeration of salmonellae in polluted waters using several modifications of existing techniques. Confirmation of salmonellae is achieved within 48 hr. This procedure includes selective enrichment in m-Tetrathionate Broth (22 ± 1 hr), plating on Brilliant Green Sulfa Agar (20 ± 1 hr), and confirmation by flagellar (H) agglutination of the growth in a mannosecontaining medium (6 ± 1 hr). An incubation temperature of 41.5 C was used throughout this procedure. Dilution to extinction techniques (most probable number) were employed to enumerate salmonellae. Large sample volumes were concentrated through the use of membrane filters. This technique proved to be rapid and reliable for the enumeration of salmonellae in water, waste water, and waste-water sludges.