Rapid resolution liquid chromatography (RRLC) analysis and studies on the stability of Shuang-Huang-Lian preparations

Ma, Yuan-Chun; Wang, Xiaoqiang; Hou, FeiFei; Ma, Jie; Luo, Mai; Chen, Alice; Jin, Peter; Lu, Shane; Xu, Iris

doi:10.1016/j.jpba.2010.08.019

Cited by 25 publications

(15 citation statements)

References 17 publications

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“…Shuang‐Huang‐Lian oral liquid (SHL) is a TCMF comprising three herbs: Scutellaria baicalensis , Forsythia suspensa and Lonicera japonica at a ratio of 1:2:1 (Chinese Pharmacopoeia Commission, ). It has been widely used in clinic for the treatment of respiratory diseases, including acute upper respiratory tract infection (Ni, Zhang, Hou, Shi, & Guo, ; Zhang et al, ), acute bronchitis (Ma et al, ) and tonsillitis with significant effect (Cao, Wang, Yu, & Ye, ). Moreover, SHL is the recommended TCMF for treatment of influenza in the Influenza Diagnosis and Treatment Guidelines (2011 edition) in China.…”

Section: Introductionmentioning

confidence: 99%

A target and nontarget strategy for identification or characterization of the chemical ingredients in Chinese herb preparation Shuang‐Huang‐Lian oral liquid by ultra‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry

Zhang

Yao

et al. 2017

Biomedical Chromatography

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A target and nontarget strategy based on in-house chemical components library was developed for rapid and comprehensive analysis of complicated components from traditional Chinese medicine preparation Shuang-Huang-Lian oral liquid. The sample was analyzed by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry using generic acquisition parameters. Automated detection and data filtering were performed on the UNIFI™ software and the detected peaks were evaluated against an in-house library. As a result, a total of 170 chemical components (110 target compounds and 60 nontarget ones) were identified or tentatively characterized, including 54 flavonoids, 30 phenylethanoid glycosides, 16 iridoid glycosides, 14 lignans, 32 organic acids, 19 triterpenoid saponins and five other types of compounds. Among them, 44 compounds were further confirmed by comparison with reference standards. It was demonstrated that this systematical approach could be successfully applied for rapid identification of multiple compounds in traditional Chinese medicine and its preparations. Furthermore, this work established the foundation for the further investigation on the metabolic fates of multiple ingredients in Shuang-Huang-Lian oral liquid.

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Section: Introductionmentioning

confidence: 99%

A target and nontarget strategy for identification or characterization of the chemical ingredients in Chinese herb preparation Shuang‐Huang‐Lian oral liquid by ultra‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry

Zhang

Yao

et al. 2017

Biomedical Chromatography

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“…Shuanghuanglian preparations(SHLs), including oral liquid, injection powder, capsule, and tablet, belong to a family of refined traditional Chinese preparation. It comprises several herbs including Flos Lonicerae, Radix Scutellariae, and Fructus Forsythiae [3][4][5] . Pharmacological action has demonstrated that it is a bacteriacide, diminishes inflammation, antiviral and is useful for treating fever, cough, pneumonia etc 6 .…”

Section: Introductionmentioning

confidence: 99%

Determination of Chlorogenic Acid, Baicalin and Forsythin in Shuanghuanglian Preparations by HPLC-Dad

Yan

et al. 2012

J. Chil. Chem. Soc.

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High performance liquid chromatography coupled with diode array detection (HPLC-DAD) was developed for the simultaneous determination of three bioactive components -chlorogenic acid, baicalin and forsythin -in traditional Chinese medicinal Shuanghuanglian preparations. The analytes were extracted successfully using 80% aqueous methanol for 15 min in an ultrasonic bath. The optical chromatographic separation conditions were performed on a Finnigan Surveyor HPLC system with an Agilent Zorbax ODS C18 (4.6×150 mm, particle size 5 µm ) at 30 o C using a gradient elution program with a mixture of acetonitrile and 0.2% phosphoric acid (v/v) as mobile phase within 25 min. Method validation results were obtained through the generation of calibration curves with the mixed standard solutions and a standard addition method, regression equations revealed linear relationships (correlation coefficients 0.9965-0.9985) between the peak area and concentration of the three analytes. The average recoveries were 90-106% (n= 6) with standard deviation below 3.37%. The developed HPLC assay was specific and could be readily utilized for comprehensive evaluation of Shuanghuanglian preparations.

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“…However, these analysis methods are either time‐consuming or require expensive equipment. In recent years, rapid resolution liquid chromatography (RRLC) has been widely used in quantitative and fingerprint analysis of natural medicines (Huang et al ., ; Qiao, Sheng, et al ., ; Ma et al ., ) because of its satisfactory separation and much shorter analysis time (Yoshida and Majors, ). Here, we present, to our knowledge, the first report on the chromatographic fingerprint analysis and simultaneous determination of six coumarins in A. dahuricae radix by the RRLC method.…”

Section: Introductionmentioning

confidence: 99%

Development of a Rapid Resolution Liquid Chromatographic Method Combined with Chemometrics for Quality Control of Angelicae dahuricae radix

Fan

Deng

Zhou

et al. 2011

Phytochemical Analysis

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Rapid resolution liquid chromatography (RRLC) analysis and studies on the stability of Shuang-Huang-Lian preparations

Cited by 25 publications

References 17 publications

A target and nontarget strategy for identification or characterization of the chemical ingredients in Chinese herb preparation Shuang‐Huang‐Lian oral liquid by ultra‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry

A target and nontarget strategy for identification or characterization of the chemical ingredients in Chinese herb preparation Shuang‐Huang‐Lian oral liquid by ultra‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry

Determination of Chlorogenic Acid, Baicalin and Forsythin in Shuanghuanglian Preparations by HPLC-Dad

Development of a Rapid Resolution Liquid Chromatographic Method Combined with Chemometrics for Quality Control of Angelicae dahuricae radix

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