Rapid reversed-phase high-performance liquid chromatography method for quantitation, at high pH, of the recombinant apolipo-protein A-IMilano in Escherichia coli fermentation broth

Mulugeta, Ezra; Carnö, Sofi; Becker, Susanne; Hagman, Anders; Mascher, Erik

doi:10.1016/s0021-9673(97)01203-x

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Protein Analysis By Perfusion Chromatography2

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“…An automated reversed-phase high-performance liquid chromatography method for quantitative determination of recombinant apolipoprotein A-IMilano (r-Apo A-IM) in E. coli fermentation broth was developed and evaluated. 322 The use of a perfusion media (Poros IIR/H) made it possible to achieve rapid separation and good resolution at high pH. The r-Apo A-IM-containing fraction was well separated from other proteins, allowing a reliable quantification.…”

Section: Protein Analysis By Perfusion Chromatographymentioning

confidence: 99%

“…Biotechnology depends on the ability to rapidly determine the quality of products produced in fermentation broths or other reactors, and the fast analysis possible with perfusion chromatography has allowed this to be done as reported in several examples. An automated reversed-phase high-performance liquid chromatography method for quantitative determination of recombinant apolipoprotein A-IMilano (r-Apo A-IM) in E. coli fermentation broth was developed and evaluated . The use of a perfusion media (Poros IIR/H) made it possible to achieve rapid separation and good resolution at high pH.…”

Section: Protein Analysis By Perfusion Chromatographymentioning

confidence: 99%

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