Rapid RP-HPLC method for simultaneous estimation of metformin, pioglitazone, and glimepiride in human plasma

Sebaiy, Mahmoud M.; El-Adl, Sobhy M.; Baraka, Mohamed M.; Hassan, Amira A.

doi:10.1556/1326.2018.00515

Cited by 29 publications

(11 citation statements)

References 21 publications

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“…The software used for analysis was UV-VIS Analyst. [18][19][20][21][22][23][24][25] The instrument used for the High Performance Liquid Chromatography consisted of binary gradient system. The software used of the HPLC instruments for the method development and the validation purpose was HPLC workstation.…”

Section: Methodsmentioning

confidence: 99%

Simultaneous estimation of pioglitazone, glimepiride & metformin hydrochloride in bulk & tablet dosage form by UV, RP-HPLC method

Shaikh

Dighe²

2021

IJPCA

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A stability-indicating UV spectroscopic and high-performance liquid chromatography (RP-HPLC) method is developed for the quantification ofPioglitazone, Glimepiride & Metformin Hydrochloride drug substances. UV spectroscopic method was developed and validated, the wavelength selected for simultaneous estimation were 226nm for pioglitazone, 229nm for glimepiride and 232nm for metformin hydrochloride. The isosbestic point found for the analysis was 229nm. Selected mobile phase was a combination of methanol and water with a ratio of 70% Methanol and 30 % HPLC water with the flow rate of 0.85ml/min. The analyte was analysed on the C18 HPLC column having the pore size of 5 microns at room temperature. The method is validated according to ICH guidelines, the retention time of about 4.0min for metformin, 5.5min for Pioglitazone and 6.8min for Glimepiride was observed. The linearity range with regression co-efficient for Pioglitazone, Glimepiride & Metformin Hydrochloride is 3-15 μg/mL,0.4-1.2 μg/mL and 100-500 μg/mL and 0.9998, 0.9991, 0.9991 respectively.

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Section: Methodsmentioning

confidence: 99%

Simultaneous estimation of pioglitazone, glimepiride & metformin hydrochloride in bulk & tablet dosage form by UV, RP-HPLC method

Shaikh

Dighe²

2021

IJPCA

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“…In addition, REM helps in reducing the body weight and blood pressure in diabetes mellitus patients. [8][9][10] Many analytical methods were illustrated for the estimation of MET from pharmaceutical preparations and plasma by spectroscopic, 11,12 HPLC, [13][14][15] HPLTC, 16 LC-MS, 17,18 and CZE, 19,20 alone and with other drugs. Few analytical procedures were also depicted in the texts for the analysis of REM.…”

Section: -Methyl-4-[[4-(1-methylethoxy)phenyl]methyl]-1-(1-mentioning

confidence: 99%

Smart UV Derivative Spectrophotometric Methods for Simultaneous Determination of Metformin and Remogliflozin: Development, Validation and Application to the Formulation

Attimarad¹,

Nair²,

Nagaraja³

et al. 2023

Ind. J. Pharm. Edu. Res

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Background:The result of pharmaceutical industry research for the new class and the new combination of drugs for the treatments of diabetes is the newly approved combination of metformin (MET) and remogliflozin (REM). For the quality control of this formulation, three smart, reproducible and non-sophisticated spectroscopic techniques were developed by modification of UV spectra. Materials and Methods: The first two methods were based on the measurements of the peak height of the third derivative and second derivative ratio spectra of MET and REM and the third method was the constant center spectrum subtraction method. Results: The proposed methods exhibited Beer's law in the range of 2.5 to 30 µg/ml and 1 to 24 µg/ml for MET and REM correspondingly by all three methods. The mean percentage recovery was found to be in the range of 99.08% to 100.15% for MET and 98.73% to 100.27% for REM. Further, both analytes were quantified from the formulation using proposed spectroscopic methods with high accuracy. Comparison of all three methods with the reported HPLC method showed no variation in the assay outcomes in relation to accuracy and precision. Conclusion: The suggested techniques are simple, accurate and reproducible, hence could be used for regular quality control of formulation consisting of MET and REM.

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“…Several methods are illustrated in the literature for the analysis of metformin alone and along with different classes of agents from formulations and body fluids. Recent methods reported were UV-spectrophotometry [ 19 , 20 , 21 ], RP-HPLC [ 22 , 23 , 24 , 25 ], HPTLC [ 24 , 25 ], capillary electrophoresis [ 26 , 27 ] and LCMS [ 28 , 29 , 30 , 31 , 32 ]. There were few reports in the literature for the simultaneous estimation of metformin along with SGLT-2 inhibitors [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Green UV Derivative Spectrophotometric Methods for Simultaneous Determination Metformin and Remogliflozin from Formulation: Evaluation of Greenness

Attimarad

Nair

Sreeharsha

et al. 2021

IJERPH

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The recent trend in green analytical chemistry is the development of green analytical methods using environmentally friendly solvents. Therefore, three ecofriendly manipulated UV spectroscopic techniques have been validated for the concurrent quantification of newly approved remogliflozin etabonate (REM) and metformin HCl (MET) tablets using water as a solvent. The first method was established using first derivative absorption spectroscopic method by determining the peak amplitude at 233.0 nm for REM and 252.2 nm for MET, a zero crossing of one the component. The second and third methods were based on the peak amplitude difference and first-order derivative absorption of the ratio spectra developed by the manipulation of scanned UV spectra. REM and MET showed good linearity in the series of 1–20 µg ml−1 and 2.5–35 µg ml−1, respectively, by all three methods with an excellent correlation coefficient (r2 ≥ 0.998). Further, the proposed UV spectroscopic techniques were validated as per International Council for Harmonization guidelines. The methods showed good sensitivity, accuracy, and precision. Anticipated procedures were effectively utilized for the concurrent quantification of REM and MET in laboratory prepared mixtures and tablets. The high percent recovery with low standard deviation found for both analytes by all three methods confirms the accuracy and precision of the procedures. Finally, the greenness of the proposed spectroscopic methods, evaluated by semi-quantitative and quantitative methods, showed the eco-friendly nature of the methods. Furthermore, the proposed approaches were simple, accurate, sensitive, economic, and environmentally friendly and hence can be utilized for regular quality control of REM and MET formulation.

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Rapid RP-HPLC method for simultaneous estimation of metformin, pioglitazone, and glimepiride in human plasma

Cited by 29 publications

References 21 publications

Simultaneous estimation of pioglitazone, glimepiride & metformin hydrochloride in bulk & tablet dosage form by UV, RP-HPLC method

Simultaneous estimation of pioglitazone, glimepiride & metformin hydrochloride in bulk & tablet dosage form by UV, RP-HPLC method

Smart UV Derivative Spectrophotometric Methods for Simultaneous Determination of Metformin and Remogliflozin: Development, Validation and Application to the Formulation

Development and Validation of Green UV Derivative Spectrophotometric Methods for Simultaneous Determination Metformin and Remogliflozin from Formulation: Evaluation of Greenness

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