Rapid screening astaxanthin-hyperproducing <i>Haematococcus pluvialis</i>
 mutants through near-infrared spectroscopy

Liu, Jinghua; Song, Lirong; Huang, Qing

doi:10.1111/lam.12531

Cited by 6 publications

(4 citation statements)

References 20 publications

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“…The use of astaxanthin biosynthesis inhibitors diphenylamine (DPA) and nicotine, or the expression of astaxanthin biosynthetic genes under week promoters can help to overcome these limitations and facilitate the screening [ 144 , 166 , 167 ]. Other screening methods using flow-cytometry and near-infrared spectroscopy have been employed to screen astaxanthin hyper-producers [ [173] , [174] , [175] ]. A high-throughput screening using an Raman-Activated Droplet Sorting (RADS) showed promising results for screening astaxanthin hyper-producers [ 176 ].…”

Section: Strategies For Microbial Cell Factories Optimizationmentioning

confidence: 99%

Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies

Basiony

Ouyang

Wang

et al. 2022

Synthetic and Systems Biotechnology

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Section: Strategies For Microbial Cell Factories Optimizationmentioning

confidence: 99%

Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies

Basiony

Ouyang

Wang

et al. 2022

Synthetic and Systems Biotechnology

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“…NIR spectroscopy can be used for quantitative analysis because it is less affected by moisture [ 58 ]. Previous studies have shown that NIR spectroscopy can be effectively utilized for analyzing algae and plant mutants, including plasma mutated Haematococcus pluvialis strains [ 59 ], space mutated tomatoes [ 60 ], and chemically mutated peanuts [ 61 ]. The NIR spectra of G .…”

Section: Resultsmentioning

confidence: 99%

Improved production of polysaccharides in Ganoderma lingzhi mycelia by plasma mutagenesis and rapid screening of mutated strains through infrared spectroscopy

Zhang

et al. 2018

PLoS ONE

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As a traditional Chinese medicine, Ganoderma lingzhi has attracted increasing attention for both scientific research and medical application. In this work, in order to improve the production of polysaccharides from an original wide-type (WT) strain (named "RWY-0") of Ganoderma lingzhi, we applied atmospheric-pressure dielectric barrier discharge (DBD) nonthermal plasma to the protoplasts of RWY-0 for mutagenesis treatment. Through a randomly amplified polymorphic DNA (RAPD) assay, at least 10 mutagenic strains were confirmed. They also showed different mycelium characteristics in terms of shape, color, size and biomass in liquid fermentation. The mutant strains were examined by infrared spectroscopy, and based on the established near-infrared (NIR) quantification model, the polysaccharide contents in these mutants were quantitatively evaluated. As a result, we found that the Ganoderma polysaccharide contents in some of the mutant strains were significantly changed compared with that of the original WT strain. The polysaccharide content of RWY-1 G. lingzhi was considerably higher than that of the WT strain, with an increase of 25.6%. Thus, this preliminary work demonstrates the extension of the plasma mutagenesis application in acquiring polysaccharide-enhanced Ganoderma lingzhi mutants and shows the usefulness of NIR spectroscopy in the rapid screening of mutagenic strains for other important ingredients.

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“…AXT is one of the most potent antioxidants known in nature, and the label‐free high‐throughput screening for this type of molecule is of particular interest for biomanufacturing applications. [ 29 ] Our system is capable of tracking the dynamic AXT accumulation process with high throughput (≈2700 events min −1 ). Thus, resonance band‐based monitoring of AXT production is greatly improved using the pDEP‐DLD‐RFC and can be employed in efforts to elevate overall system productivity (e.g., monitoring the microalgal cultivation to synchronize cells to a high‐AXT state).…”

Section: Discussionmentioning

confidence: 99%

Robust Spontaneous Raman Flow Cytometry for Single‐Cell Metabolic Phenome Profiling via pDEP‐DLD‐RFC

et al. 2023

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A full-spectrum spontaneous single-cell Raman spectrum (fs-SCRS) captures the metabolic phenome for a given cellular state of the cell in a label-free, landscape-like manner. Herein a positive dielectrophoresis induced deterministic lateral displacement-based Raman flow cytometry (pDEP-DLD-RFC) is established. This robust flow cytometry platform utilizes a periodical positive dielectrophoresis induced deterministic lateral displacement (pDEP-DLD) force that is exerted to focus and trap fast-moving single cells in a wide channel, which enables efficient fs-SCRS acquisition and extended stable running time. It automatically produces deeply sampled, heterogeneity-resolved, and highly reproducible ramanomes for isogenic cell populations of yeast, microalgae, bacteria, and human cancers, which support biosynthetic process dissection, antimicrobial susceptibility profiling, and cell-type classification. Moreover, when coupled with intra-ramanome correlation analysis, it reveals state-and cell-type-specific metabolic heterogeneity and metabolite-conversion networks. The throughput of ≈30-2700 events min −1 for profiling both nonresonance and resonance marker bands in a fs-SCRS, plus the >5 h stable running time, represent the highest performance among reported spontaneous Raman flow cytometry (RFC) systems. Therefore, pDEP-DLD-RFC is a valuable new tool for label-free, noninvasive, and high-throughput profiling of single-cell metabolic phenomes.

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Rapid screening astaxanthin-hyperproducing Haematococcus pluvialis mutants through near-infrared spectroscopy

Cited by 6 publications

References 20 publications

Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies

Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies

Improved production of polysaccharides in Ganoderma lingzhi mycelia by plasma mutagenesis and rapid screening of mutated strains through infrared spectroscopy

Robust Spontaneous Raman Flow Cytometry for Single‐Cell Metabolic Phenome Profiling via pDEP‐DLD‐RFC

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