Rapid screening of Aspergillus terreus mutants for overproduction of lovastatin

Ferron, M.; López, J.L. Casas; Pérez, J.A. Sánchez; Sevilla, J. M. Fernández; Chisti, Yusuf

doi:10.1007/s11274-004-3045-z

Cited by 30 publications

(10 citation statements)

References 11 publications

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“…It is likely that an inhibition zone was produced because A. terreus secrets metabolites that interfere with the growth of P. aphanidermatum . Ferrón et al (2005) and Wang et al (2011) reported the production of terremides A and B and lovastatin by A. terreus that were found responsible for the production of inhibition zones. In our study, the use of CF from A. terreus isolates resulted in electrolyte leakage in P. aphanidermatum mycelium and also interfered with oospore production in P. aphanidermatum .…”

Section: Discussionmentioning

confidence: 99%

Aspergillus terreus Inhibits Growth and Induces Morphological Abnormalities in Pythium aphanidermatum and Suppresses Pythium-Induced Damping-Off of Cucumber

2018

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The study investigated the efficacy of two isolates of Aspergillus terreus (65P and 9F) on the growth, morphology and pathogenicity of Pythium aphanidermatum on cucumber. In vitro tests showed that the two isolates inhibited the growth of P. aphanidermatum in culture. Investigating P. aphanidermatum hyphae close to the inhibition zone showed that the hyphae showed abnormal growth and loss of internal content. Treating P. aphanidermatum with the culture filtrate (CF) of A. terreus resulted in significant rise in cellular leakage of P. aphanidermatum mycelium. Testing glucanase enzyme activity by both A. terreus isolates showed a significant increase in glucanase activity. This suggests that the cell walls of Pythium, which consist of glucan, are affected by the glucanase enzyme produced by A. terreus. In addition, Aspergillus isolates produced siderephore, which is suggested to be involved in inhibition of Pythium growth. Also, the CFs of 65P and 9F isolates significantly reduced spore production by P. aphanidermatum compared to the control (P < 0.05). In bioassay tests, the two isolates of A. terreus increased the survival rate of cucumber seedlings from 10 to 20% in the control seedlings treated with P. aphanidermatum to 38–39% when the biocontrol agents were used. No disease symptoms were observed on cucumber seedlings only treated with the isolates 65P and 9F of A. terreus. In addition, the A. terreus isolates did not have any negative effects on the growth of cucumber seedlings. This study shows that isolates of A. terreus can help suppress Pythium-induced damping-off of cucumber, which is suggested to be through the effect of A. terreus and its glucanase enzyme on P. aphanidermatum mycelium.

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Section: Discussionmentioning

confidence: 99%

Aspergillus terreus Inhibits Growth and Induces Morphological Abnormalities in Pythium aphanidermatum and Suppresses Pythium-Induced Damping-Off of Cucumber

2018

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“…Most of the methods used for the hyper-production of statins in A. terreus species include: Chemical mutagenesis, involving use of mutagenic chemicals such as ethyl methanesulfonate (EMS) and N -methyl- N ’-nitro- N -nitrosoguanidine (NTG) [ 45 , 82 , 83 ] and Physical mutagenesis, involving the use of radiation such as high radiation heavy ion beams and ultraviolet radiation [ 57 , 71 ]. …”

Section: Exploitation Of a Terreus For Statin mentioning

confidence: 99%

“…Chemical mutagenesis, involving use of mutagenic chemicals such as ethyl methanesulfonate (EMS) and N -methyl- N ’-nitro- N -nitrosoguanidine (NTG) [ 45 , 82 , 83 ] and…”

Section: Exploitation Of a Terreus For Statin mentioning

confidence: 99%

Exploitation of Aspergillus terreus for the Production of Natural Statins

Subhan

Faryal

Macreadie

2016

JoF

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The fungus Aspergillus (A.) terreus has dominated the biological production of the “blockbuster” drugs known as statins. The statins are a class of drugs that inhibit HMG-CoA reductase and lead to lower cholesterol production. The statins were initially discovered in fungi and for many years fungi were the sole source for the statins. At present, novel chemically synthesised statins are produced as inspired by the naturally occurring statin molecules. The isolation of the natural statins, compactin, mevastatin and lovastatin from A. terreus represents one of the great achievements of industrial microbiology. Here we review the discovery of statins, along with strategies that have been applied to scale up their production by A. terreus strains. The strategies encompass many of the techniques available in industrial microbiology and include the optimization of media and fermentation conditions, the improvement of strains through classical mutagenesis, induced genetic manipulation and the use of statistical design.

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“…The bioassay method for screening the lovastatin producing fungi was adopted from Kumar et al (2000) [25] and Vilches et al (2005) [26] using Saccharomyces cerevisiae as indicator organisms, and the ability of lovastatin to inhibit fungal growth was tested. Yeast growth inhibition was performed by seeding 15 ml of glucose yeast peptone agar medium with 0.25 ml of yeast culture (S. cerevisiae).…”

Section: Lovastatin Detectionmentioning

confidence: 99%

Purification and Cytotoxicity Study of Lovastatin From Soil Fungi

Balraj

Saibaba

Archunan

et al. 2017

Asian J Pharm Clin Res

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Objective: The objective of the present study is to evaluate the anticancer potential of lovastatin obtained from fungal source.Methods: About 15 fungal cultures were isolated from soil samples collected from Bharathiar University, India, and all are identified and characterized through microscopic characterization. Lovastatin producing capability was confirmed through bioassay against Saccharomyces cerevisiae, and the ability of selected fungus to produce lovastatin was further confirmed by high-performance liquid chromatography. Maximum lovastatin producing fungi were further selected for purification (overloaded elution chromatography) and characterization done using inhibition rate (IR). 5-diphenyl tetrazolium bromide (MTT) assay using A549 cell line was performed for antitumor activity evaluation.Results: Among the 15 isolates, Aspergillus flavus exhibited the maximum zone of inhibition (1.5 cm) against the test organism through solid-state fermentation. The resemblance in retention time (RT) of peak shown in chromatograms of standard lovastatin (RT=25.1 minutes) and sample (RT=25.1 minutes) were similar. This confirmed the presence of lovastatin in the selected fungal isolate (A. flavus). The presence of two functional groups in lovastatin C=O and O-H was confirmed by IR spectrum 50% of cell lysis was observed in MTT assay.Conclusion: Lovastatin obtained from soil fungi is capable of producing lovastatin in good proportions. Obtained fungal lovastatin exhibited significant antitumor activity against A549 cell line. Like other biological derivatives, lovastatin from soil fungi had greater potential in anticancer activity, and further biosynthetic pathway insights in their production can improve the yield which aid in large scale production.

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Rapid screening of Aspergillus terreus mutants for overproduction of lovastatin

Cited by 30 publications

References 11 publications

Aspergillus terreus Inhibits Growth and Induces Morphological Abnormalities in Pythium aphanidermatum and Suppresses Pythium-Induced Damping-Off of Cucumber

Aspergillus terreus Inhibits Growth and Induces Morphological Abnormalities in Pythium aphanidermatum and Suppresses Pythium-Induced Damping-Off of Cucumber

Exploitation of Aspergillus terreus for the Production of Natural Statins

Purification and Cytotoxicity Study of Lovastatin From Soil Fungi

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