Rapid screening of embryonated chicken eggs for bluetongue virus infection with an antigen capture enzyme linked immunosorbent assay

Hosseini, Mohammad Hossein; Hawkes, R. A.; Kirkland, PD; Dixon, Robert

doi:10.1016/s0166-0934(98)00096-2

Cited by 8 publications

(6 citation statements)

References 9 publications

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“…In this study, the MAb was the capture antibody and rabbit HIS against rVP7 was the detection antibody and is in agreement with the previous reports [6,23] . After optimizing the dilutions of the reagents, viz.…”

Section: Characterization Of Mab Clonessupporting

confidence: 91%

Detection of bluetongue virus group-specific antigen using monoclonal antibody based sandwich ELISA

et al. 2010

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A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for use in the sandwich ELISA. The MAb, designated as 5B5 was specific to VP7 and belongs to IgG2a subclass and was selected for the development of the sELISA in this study. The MAb had a titer of 1:25 with BTV and 1:2 with the rVP7 protein. The sELISA is based on capturing of BTV antigen with VP7 specific MAb followed by detection using BTV polyclonal antiserum raised in rabbits. The assay was evaluated with six cell culture adapted serotypes of BTV that have been isolated from India, 1, 2, 15, 17, 18 and 23. The assay could detect BTV antigen as early as day 8 in blood. It was also successfully applied for the detection of BTV group specific antigen in clinical samples of blood, washed RBCs, buffy coat and plasma. A total of 102 field samples from animals, suspected of being infected with BTV, were tested and 29.42% were positive. The blood samples were also amplified in cell culture which improved the sensitivity of the assay. Results confirmed that the sELISA is rapid and specific.

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Section: Characterization Of Mab Clonessupporting

confidence: 91%

Detection of bluetongue virus group-specific antigen using monoclonal antibody based sandwich ELISA

et al. 2010

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“…Virus isolation on selected semi‐quantitative real‐time polymerase chain reaction (qRT‐PCR) positive liver homogenates from midge transmission experiments described below was performed following the methods described by Gard & Kirkland () and Hosseini et al . ().…”

Section: Methodsmentioning

confidence: 97%

“…Embryonated chicken eggs used for infection (donor hosts) and transmission (recipient hosts) experiments were obtained from a commercial supplier. Donor ECEs were used at the age of 10–11 days and inoculated with BTV via the intravascular (IV) route as described previously (Gard & Kirkland, ; Hosseini et al ., ). Recipient ECEs used for the transmission tests were 12 days old at the time of feeding.…”

Section: Methodsmentioning

confidence: 97%

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Application of an embryonated chicken egg model to assess the vector competence of Australian Culicoides midges for bluetongue viruses

Saag

Ward

Kirkland

2017

Medical Vet Entomology

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Culicoides biting midges (Diptera: Ceratopogonidae) are vectors of a number of globally important arboviruses that affect livestock, including bluetongue virus (BTV), African horse sickness virus and the recently emerged Schmallenberg virus. In this study, a model using embryonated chicken eggs (ECEs) was utilized to undertake vector competence studies of Australian Culicoides spp. for 13 laboratory-adapted or wild-type virus strains of BTV. A total of 7393 Culicoides brevitarsis were reared from bovine dung, and 3364 Culicoides were induced to feed from ECEs infected with different strains of BTV. Of those, 911 (27%) survived the putative extrinsic incubation period of 9-12 days. In some trials, virus was also transmitted onward to uninfected ECEs, completing the transmission cycle. This model does not rely on the use of colonized midges and has the capacity to assess the vector competence of field-collected insects with strains of virus that have not previously been passaged in laboratory culture systems. There is also potential for this model to be used in investigations of the competence of Culicoides spp. for other arboviruses.

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“…After the final wash, the equal volume of OPG medium was added in all the blood samples. The red blood cells were haemolysed as per the method described by Hosseini et al (1998). After this, the blood samples were transferred in screw capped vials and stored at 4°C until further use.…”

Section: Methodsmentioning

confidence: 99%

Seroepidemiology, molecular and entomological studies of bluetongue in sheep in Gujarat

Chauhan¹,

Bhagat²,

Dadawala³

et al. 2016

Afr. J. Microbiol. Res.

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Rapid screening of embryonated chicken eggs for bluetongue virus infection with an antigen capture enzyme linked immunosorbent assay

Cited by 8 publications

References 9 publications

Detection of bluetongue virus group-specific antigen using monoclonal antibody based sandwich ELISA

Detection of bluetongue virus group-specific antigen using monoclonal antibody based sandwich ELISA

Application of an embryonated chicken egg model to assess the vector competence of Australian Culicoides midges for bluetongue viruses

Seroepidemiology, molecular and entomological studies of bluetongue in sheep in Gujarat

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