Rapid, sensitive and cost-effective determination of immune checkpoint inhibitor activity using a magnetic bead-based binding assay

Meng, Qian‐Fang; Fu, Yujia; Li, Shenzhi; Yan, Yujuan; Duan, Danli; Anthony, Donald D.; Zhu, Yueping; Wu, Xuehua; Qian, Feng; Wu, Changxin

doi:10.1016/j.jim.2021.113134

Cited by 1 publication

(1 citation statement)

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“…Immunoaffinity-based SPE has been introduced in the past decades, which employs sorbents covalently attached with antibodies of the aimed analytes. Particularly, immunomagnetic beads exploit the benefits of both magnetic and immunoaffinity-based SPE, enabling efficient, environmentally friendly and readily scalable extraction from large-volume samples. , For instance, Rehm et al reported poly-3-hydroxybutyrate inclusions densely coated with ferrofluid and specific antibodies for isolation and electrochemical detection of cancer biomarkers . This technique was firstly described in biological matrices including blood and urine, where the atibody–antigen binding potency was optimized .…”

Section: Introductionmentioning

confidence: 99%

Magnetic Liposomes Infused with GPCR-Expressing Cell Membrane for Targeted Extraction Using Minimum Organic Solvent: An Investigative Study of Trace THC in Sewage

et al. 2023

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Trace analysis of lipophilic substances in complex environmental, food, or biological matrices has proven to be a challenge, on account of their high susceptibility to adsorption by particulate matter and liquid–solid interfaces. For this purpose, liquid–liquid extraction (LLE) is often employed as the separation method, which uses water-immiscible organic solvents. As an alternative, magnetic solid-phase extraction (MSPE) allows for adsorption, separation, and recovery of analytes from large volumes of aqueous samples with minimum usage of organic solvents. However, the poor selectivity hampers its performance in various scenarios, especially in sewage samples where complicated and unpredictable interference exists, resulting in block of the active adsorption sites of the sorbent. To this end, we propose receptor-affinity MSPE employing magnetic liposomes decorated with cell membranes expressing G-protein-coupled receptor as the sorbents. Application of the novel sorbent CM@Lip@Fe infused with CB1 cannabinoid receptors was demonstrated for the targeted extraction and enrichment of tetrahydrocannabinol from sewage matrix. Thanks to the high affinity and molecular selectivity of the ligand-receptor interactions, a limit of quantitation of 5.17 ng/L was achieved coupled with HPLC-MS/MS in unfiltered raw sewage, featuring minimum usage of organic solvents, fivefold enhanced sensitivity, low sorbent dosage (75 mg/L of sewage), and high efficiency as major advantages over conventional LLE. This work establishes a framework for efficient separation of specific molecules from complex media, thus promising to extend and refine standard LLE as the clean-up procedure for trace analysis.

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