Rapid, specific, simple, in-field detection ofXanthomonas campestrispathovarmusacearumby loop-mediated isothermal amplification

Abstract

This is the first LAMP assay for Xcm which provides a significant improvement compared to existing diagnostics.

A diagnostic tool for improved detection of Xanthomonas fragariae using a rapid and highly specific LAMP assay designed with comparative genomics

“…The sensitivity of the LAMP assay with genomic DNA of X. fragariae was 10 2 fg, which was equal to the sensitivity of the LAMP assay designed for X. campestris pv . musacearum (Hodgetts et al ., ). Nevertheless, the sensitivity obtained is slightly lower than assays designed for other Xanthomonas pathovars (Bühlmann et al ., 2013b; Lang et al ., ), Pseudomonas pathovars (Li et al ., ; Ruinelli et al ., ) and Erwinia amylovora (Bühlmann et al ., 2013a).…”

“…Because the reaction is isothermal, a LAMP protocol can be performed in the field using a simple water bath or a heating block with fluorescence reader (Okuda et al ., ; Tomlinson, ). This technological advantage of LAMP could reduce the time between sampling and sample analysis and is therefore attractive for regulatory laboratories and quarantine officials (Lang et al ., ; Hodgetts et al ., ); it is also useful for deployment in nonspecialized laboratories using a portable detection device that is simple to operate (Bühlmann et al ., 2013a).…”

Xanthomonas campestris pv. musacearum: a major constraint to banana, plantain and enset production in central and east Africa over the past decade

“…Hodgetts et al . () developed and evaluated a loop‐mediated isothermal amplification (LAMP) assay for Xcm diagnosis. The LAMP primers target the general secretion pathway protein D gene and are able to detect Xcm DNA at a concentration as low as 51 fg, providing a level of sensitivity much greater than that of an ordinary Xcm PCR assay.…”

Evaluation of a loop-mediated isothermal amplification assay based onhrpZgene for rapid detection and identification ofPseudomonas syringaepv.lachrymansin cucumber leaves

“…), not only due to the rapid and sensitive detection provided, but also due to the wide range of detection strategies available and the ability of the technique to be deployed away from the conventional laboratory setting (Hodgetts et al . ; Niu et al . ).…”