1958
DOI: 10.2134/agronj1958.00021962005000040012x
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Rapid Tests for Free and Bound Coumarin in Sweetclover1

Abstract: Synopsis Two qualitative fluorometric methods are described. The tube test, in which scoring is based upon visual comparison of the fluorescence of test solutions with the fluorescence of standards, was more reliable than the paper test. The paper test should be used primarily for rapid, initial screening of large populations.

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Cited by 6 publications
(2 citation statements)
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“…The paper test employed was similar to that previously described for unheated samples (6), except that a drop of [:I-glucosidase preparation was added to each sample spot as soon as an entire sheet of samples had been taken. This procedure insured the hydrolysis of cis-a·hydroxycinnamic acid glucoside in those samples lacking endogenous [:I-glucosidase activity, and thus permitted the accurate classification of Cuand cucu plants regardless of constitution with respect to the B/b alleles.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The paper test employed was similar to that previously described for unheated samples (6), except that a drop of [:I-glucosidase preparation was added to each sample spot as soon as an entire sheet of samples had been taken. This procedure insured the hydrolysis of cis-a·hydroxycinnamic acid glucoside in those samples lacking endogenous [:I-glucosidase activity, and thus permitted the accurate classification of Cuand cucu plants regardless of constitution with respect to the B/b alleles.…”
Section: Methodsmentioning
confidence: 99%
“…This procedure also was similar to that previously described for unheated samples (6), except that a drop of sweetclover leaf extract containing cis-o-hydroxycinnamic acid glucoside (approximately I mg/ml) was added to the sample spot. By this means, B-plants were readily distinguished from bb plants.…”
Section: Methodsmentioning
confidence: 99%