Rapid Tuberculosis Diagnosis Using Reporter Enzyme Fluorescence

Sule, Preeti; Tilvawala, Ronak; Mustapha, Toriq; Hassounah, Hany; Noormohamed, Aneesa; Kundu, Suprateek; Graviss, Edward A.; Walkup, Grant K.; Kong, Ying; Cirillo, Jeffrey D.

doi:10.1128/jcm.01462-19

Cited by 10 publications

(10 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, it has been suggested that unknown β-lactamase present in the clinical samples might be cleaved by the fluorogenic substrate because the hypothetical enzyme could have a similar active site to that of the M. tuberculosis enzyme [47].…”

Section: Detection Of M Tuberculosismentioning

confidence: 99%

“…However, sputum may interfere during the detection of this enzyme, increasing the probability of false-positive results. Hence, it has been suggested that unknown β-lactamase present in the clinical samples might be cleaved by the fluorogenic substrate because the hypothetical enzyme could have a similar active site to that of the M. tuberculosis enzyme [ 47 ].…”

Section: Detection Of M Tuberculosismentioning

confidence: 99%

See 1 more Smart Citation

Revisiting the methods for detecting Mycobacterium tuberculosis: what has the new millennium brought thus far?

Campelo

Sousa

Nogueira

et al. 2021

Access Microbiology

View full text Add to dashboard Cite

Tuberculosis (TB) affects around 10 million people worldwide in 2019. Approximately 3.4 % of new TB cases are multidrug-resistant. The gold standard method for detecting Mycobacterium tuberculosis , which is the aetiological agent of TB, is still based on microbiological culture procedures, followed by species identification and drug sensitivity testing. Sputum is the most commonly obtained clinical specimen from patients with pulmonary TB. Although smear microscopy is a low-cost and widely used method, its sensitivity is 50–60 %. Thus, owing to the need to improve the performance of current microbiological tests to provide prompt treatment, different methods with varied sensitivity and specificity for TB diagnosis have been developed. Here we discuss the existing methods developed over the past 20 years, including their strengths and weaknesses. In-house and commercial methods have been shown to be promising to achieve rapid diagnosis. Combining methods for mycobacterial detection systems demonstrates a correlation of 100 %. Other assays are useful for the simultaneous detection of M. tuberculosis species and drug-related mutations. Novel approaches have also been employed to rapidly identify and quantify total mycobacteria RNA, including assessments of global gene expression measured in whole blood to identify the risk of TB. Spoligotyping, mass spectrometry and next-generation sequencing are also promising technologies; however, their cost needs to be reduced so that low- and middle-income countries can access them. Because of the large impact of M. tuberculosis infection on public health, the development of new methods in the context of well-designed and -controlled clinical trials might contribute to the improvement of TB infection control.

show abstract

Section: Detection Of M Tuberculosismentioning

confidence: 99%

Section: Detection Of M Tuberculosismentioning

confidence: 99%

Revisiting the methods for detecting Mycobacterium tuberculosis: what has the new millennium brought thus far?

Campelo

Sousa

Nogueira

et al. 2021

Access Microbiology

View full text Add to dashboard Cite

show abstract

“…The animals were maintained with commercial diet and water ad libitum. The guinea pigs were divided into two groups of four, one group underwent aerosol infection with Mtb strain CDC1551, grown as described previously [ 41 , 42 ] and washed with saline and suspended at an OD 600 of 0.1 prior to nebulization using a Madison chamber, as described previously [ 41 , 43 ]. The other group was used as a control without any infection procedure.…”

Section: Methodsmentioning

confidence: 99%

Increased DNA methylation, cellular senescence and premature epigenetic aging in guinea pigs and humans with tuberculosis

Bobak

Abhimanyu

Natarajan

et al. 2022

Aging

Self Cite

View full text Add to dashboard Cite

Background: Tuberculosis (TB) is the archetypical chronic infection, with patients having months of symptoms before diagnosis. In the two years after successful therapy, survivors of TB have a three-fold increased risk of death. Methods: Guinea pigs were infected with Mycobacterium tuberculosis (Mtb) for 45 days, followed by RRBS DNA methylation analysis. In humans, network analysis of differentially expressed genes across three TB cohorts were visualized at the pathway-level. Serum levels of inflammation were measured by ELISA. Horvath (DNA methylation) and RNA-seq biological clocks were used to investigate shifts in chronological age among humans with TB. Results: Guinea pigs with TB demonstrated DNA hypermethylation and showed system-level similarity to humans with TB (p-value = 0.002). The transcriptome in TB in multiple cohorts was enriched for DNA methylation and cellular senescence. Senescence associated proteins CXCL9, CXCL10, and TNF were elevated in TB patients compared to healthy controls. Humans with TB demonstrate 12.7 years (95% CI: 7.5, 21.9) and 14.38 years (95% CI: 10.23-18.53) of cellular aging as measured by epigenetic and gene expression based cellular clocks, respectively. Conclusions: In both guinea pigs and humans, TB perturbs epigenetic processes, promoting premature cellular aging and inflammation, a plausible means to explain the long-term detrimental health outcomes after TB.

show abstract

“…The CDG-3 probe has high selectivity to BlaC, and is used to measure the levels of enzymes and M. tuberculosis in sputum. The sensitivity and specificity for CDG-3 according to the ROC curve were 88.1 and 86.1% in 160 clinical specimens from potential TB patients, with a negative predictive value of 93%; thus, it could be used to predict potential TB patients ( Sule et al, 2019 ). BlaC is secreted by the Tat secretion system in live M. tuberculosis ( McDonough et al, 2005 ); thus, the BlaC-specific reporter enzyme fluorescence test is suitable for the evaluation of the treatment results and phenotypic drug susceptibility.…”

Section: New Promising Diagnostic Techniquesmentioning

confidence: 99%

Improved Conventional and New Approaches in the Diagnosis of Tuberculosis

Dong

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Tuberculosis (TB) is a life-threatening infectious disease caused by Mycobacterium tuberculosis (M. tuberculosis). Timely diagnosis and effective treatment are essential in the control of TB. Conventional smear microscopy still has low sensitivity and is unable to reveal the drug resistance of this bacterium. The traditional culture-based diagnosis is time-consuming, since usually the results are available after 3–4 weeks. Molecular biology methods fail to differentiate live from dead M. tuberculosis, while diagnostic immunology methods fail to distinguish active from latent TB. In view of these limitations of the existing detection techniques, in addition to the continuous emergence of multidrug-resistant and extensively drug-resistant TB, in recent years there has been an increase in the demand for simple, rapid, accurate and economical point-of-care approaches. This review describes the development, evaluation, and implementation of conventional diagnostic methods for TB and the rapid new approaches for the detection of M. tuberculosis.

show abstract

Rapid Tuberculosis Diagnosis Using Reporter Enzyme Fluorescence

Cited by 10 publications

References 71 publications

Revisiting the methods for detecting Mycobacterium tuberculosis: what has the new millennium brought thus far?

Revisiting the methods for detecting Mycobacterium tuberculosis: what has the new millennium brought thus far?

Increased DNA methylation, cellular senescence and premature epigenetic aging in guinea pigs and humans with tuberculosis

Improved Conventional and New Approaches in the Diagnosis of Tuberculosis

Contact Info

Product

Resources

About