2019
DOI: 10.1371/journal.pbio.3000291
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Rapid ultrasensitive detection platform for antimicrobial susceptibility testing

Abstract: Rapid detection and phenotyping of pathogenic microbes is critical for administration of effective antibiotic therapies and for impeding the spread of antibiotic resistance. Here, we present a novel platform, rapid ultrasensitive detector (RUSD), that utilizes the high reflectance coefficient at high incidence angles when light travels from low- to high-refractive-index media. RUSD leverages a principle that does not require complex manufacturing, labeling, or processing steps. Utilizing RUSD, we can detect ex… Show more

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Cited by 22 publications
(21 citation statements)
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“…However, this method requires a longer testing time than our study. Cansizoglu et al [35] introduced a novel platform for rapid ultra-sensitive detection of antimicrobial susceptibility. In that study, the minimum inhibitory concentration of bacteria was measured within 2 to 4 h by using a rapid ultra-sensitive detector (RUSD) utilizing changes in refractive index between two media with a different refractive angle.…”
Section: Discussionmentioning
confidence: 99%
“…However, this method requires a longer testing time than our study. Cansizoglu et al [35] introduced a novel platform for rapid ultra-sensitive detection of antimicrobial susceptibility. In that study, the minimum inhibitory concentration of bacteria was measured within 2 to 4 h by using a rapid ultra-sensitive detector (RUSD) utilizing changes in refractive index between two media with a different refractive angle.…”
Section: Discussionmentioning
confidence: 99%
“…Any delay in appropriate antimicrobial therapy can lead to increased mortality for severe infections (Delaloye and Calandra, 2014 ; Liu et al, 2017 ). There is, therefore, an urgent need for more rapid AST (Sanguinetti and Posteraro, 2017 ; Kim et al, 2018 ; Cansizoglu et al, 2019 ; Idelevich and Becker, 2019 ).…”
Section: Antimicrobial Susceptibility Testing Methods For Existing CLmentioning
confidence: 99%
“…Suitable methods include multiplex TaqMan PCR for specific antibiotic resistance genes for a target panel of the most prevalent pathogens and next generation sequencing technologies such as MinION nanopore sequencing that offer real-time sequencing to rapidly identify bacterial ID and drug resistance profiles 37 40 . Single cell AST could also elucidate variability in susceptibility phenotypes not often detectable and discernable in bulk populations 41 , 42 .…”
Section: Resultsmentioning
confidence: 99%