Ras suppressor 1 long form (RSU1L) silencing promotes apoptosis in invasive breast cancer cells

Christou, Christiana; Christodoulou, Maria-Ioanna; Zaravinos, Apostolos; Gkretsi, Vasiliki

doi:10.1016/j.cellsig.2022.110522

Cited by 3 publications

(3 citation statements)

References 33 publications

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“…In breast cancer studies, Vasaturo et al [ 24 ] demonstrated that overexpression of RSU1 in Michigan Cancer Foundation-7 (MCF-7) breast cancer cells induced p21 activation and reduced cancer cell proliferation by inhibiting cyclin-dependent kinase, suggesting that RSU1 acts as a tumor suppressor gene in breast cancer, but another study revealed that RSU1 is upregulated at both mRNA and protein levels in more aggressive and highly aggressive MDA-MB-231 breast cancer cells compared to non-invasive MCF-7 breast cancer cells[ 25 ]. Consistent with the above study, Christou et al [ 26 ] reported that high RSU1 expression is associated with poor breast cancer survival. In hepatocellular carcinoma, RSU1 expression has also been found to be elevated in more aggressive HepG2 hepatocellular carcinoma cells compared to non-metastatic Alexander cells[ 13 ], and RSU1 promotes invasion of hepatocellular carcinoma, similar to breast cancer cells.…”

Section: Discussionsupporting

confidence: 79%

Comprehensive analysis of cell-extracellular matrix protein Ras suppressor-1 in function and prognosis of gastrointestinal cancers

Xu,

Hou,

et al. 2023

World J Methodol

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The documentation of psychological trauma is obviously a challenge to clinicians while they are diving deep into remote events related to their clients or patients. The potential role of psychological trauma in the early developmental stages, and even the existence of adverse childhood experiences, is important to prove, yet it is difficult to do so. A diverse range of methods have been applied, all of which presumably benchmark a big therapeutic step; however, these enthusiastic methods frequently do not last for long. While hypnosis supporters, Freudian and Neo-Freudian disciples can be acute enough to enhance and uncover suppressed memories, modern psychiatry relies mostly on diversely structured interviews. Functional magnetic resonance and its related subtleties might help, but the questions that remain unanswered are numerous and confusing. Connecting early experiences with long-term memory while identifying psychological trauma its importance for the individual’s growth trajectory; thus, it remains an intriguing issue.

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Section: Discussionsupporting

confidence: 79%

Comprehensive analysis of cell-extracellular matrix protein Ras suppressor-1 in function and prognosis of gastrointestinal cancers

Xu,

Hou,

et al. 2023

World J Methodol

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“…Although direct evidence indicating the unique functions of these transcript isoforms in fatty liver disease is limited, some studies related to the transcripts indirectly suggested their possible disease-related roles. For instance, the chicken ENSGALT00010010626 and human ENST00000345264 transcript isoforms of RSU1 might have distinct functions in Ras protein activity because of their additional exon expressions [ 40 ]. When downregulated in fatty liver conditions, these isoforms could contribute to the disease’s characteristics.…”

Section: Discussionmentioning

confidence: 99%

Cross-species analysis of differential transcript usage in humans and chickens with fatty liver disease

Chokeshaiusaha,

Sananmuang,

Puthier

et al. 2023

Vet World

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Background and Aim: Fatty liver disease is a common condition, characterized by excess fat accumulation in the liver. It can contribute to more severe liver-related health issues, making it a critical concern in avian and human medicine. Apart from modifying the gene expression of liver cells, the disease also alters the expression of specific transcript isoforms, which might serve as new biological markers for both species. This study aimed to identify cross-species genes displaying differential expressions in their transcript isoforms in humans and chickens with fatty liver disease. Materials and Methods: We performed differential gene expression and differential transcript usage (DTU) analyses on messenger RNA datasets from the livers of both chickens and humans with fatty liver disease. Using appropriate cross-species gene identification methods, we reviewed the acquired candidate genes and their transcript isoforms to determine their potential role in fatty liver disease’s pathogenesis. Results: We identified seven genes - ALG5, BRD7, DIABLO, RSU1, SFXN5, STIMATE, TJP3, and VDAC2 - and their corresponding transcript isoforms as potential candidates (false discovery rate ≤0.05). Our findings showed that these genes most likely contribute to fatty disease development and progression. Conclusion: This study successfully identified novel human-chicken DTU genes in fatty liver disease. Further research is encouraged to verify the functions and regulations of these transcript isoforms as potential diagnostic markers for fatty liver disease in humans and chickens. Keywords: cross-species biomarkers, differential gene expression, differential transcript usage, fat metabolism, fatty liver disease, lipidosis, transcript isoforms.

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“…Ethanolic extracts were weighted and dissolved in 0.1% Dimethyl Sulfoxide (DMSO) (diluted with PBS) to obtain stock solutions with concentrations ranging from 200-1000 µg/mL. Cell viability following treatment with the plant extracts was assessed using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay [23]. Briefly, MDA-MB231, HeLa, SKOV3, HT-29, and HCT-116 cells were seeded in triplicate in 96-well plates at a density of 10 4 cells/well.…”

Section: Mtt Assaymentioning

confidence: 99%

Phytochemical Profiles and Biological Activities of Plant Extracts from Aromatic Plants Cultivated in Cyprus

Chrysargyris,

Petrovic,

Tomou

et al. 2024

Biology

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Medicinal and aromatic plants’ properties, still an interesting research area, are attributed to the presence of various specialized products that possess important pharmacological activities. In the present study, six medicinal/aromatic plants (Sideritis cypria, Origanum dubium, Melissa officinalis, Mentha piperita, Thymus capitatus, and Salvia fruticosa) were evaluated for their phytochemical and nutritive composition, as well as their biological activities, including antioxidant, antimicrobial, and cytotoxic properties. The results obtained indicate that M. piperita was rich in proteins and minerals such as N and Mg, while S. cypria accumulated more K, Na, P, and Ca. The highest content of phenols and flavonoids was observed in M. piperita, followed by O. dubium and T. capitatus, which eventually influenced their high antioxidant capacity. NMR screening revealed the presence of (i) triterpenoids and hydroxycinnamic acid derivatives in M. officinalis; (ii) terpenoids, flavonoids, and phenolic acid derivatives in S. fruticosa; (iii) flavonoids and phenolic acid derivatives in M. piperita; (iv) phenolic monoterpenes in O. dubium and T. capitatus; and (v) terpenoids, flavones, and phenylethanoid glycosides in S. cypria. The results of the antimicrobial activity showed that the tested samples overall had quite good antimicrobial potential. High antibacterial activity was found in O. dubium and T. capitatus, while O. dubium and S. cypria exhibited great antifungal activities. The studied species also had an important effect on the viability of female-derived and colon cancer cells. In particular, in colon cancer cells, the extracts from T. capitatus, M. officinalis, M. piperita, and S. fruticosa exhibited a stronger effect on cell viability in the more metastatic cell line at significantly lower concentrations, indicating an important therapeutic potential in targeting highly metastatic tumors. This finding is worth further investigation. The present study unveiled interesting phytochemical profiles and biological properties of the six medicinal/aromatic plants, which should be further explored, contributing to green chemistry and the possible creation of natural health products for humans’ health/nutrition and additives in cosmetics.

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Ras suppressor 1 long form (RSU1L) silencing promotes apoptosis in invasive breast cancer cells

Cited by 3 publications

References 33 publications

Comprehensive analysis of cell-extracellular matrix protein Ras suppressor-1 in function and prognosis of gastrointestinal cancers

Comprehensive analysis of cell-extracellular matrix protein Ras suppressor-1 in function and prognosis of gastrointestinal cancers

Cross-species analysis of differential transcript usage in humans and chickens with fatty liver disease

Phytochemical Profiles and Biological Activities of Plant Extracts from Aromatic Plants Cultivated in Cyprus

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