RasI/R Quorum Sensing System Controls the Virulence of
            <i>Ralstonia solanacearum</i>
            Strain EP1

Yan, Jinli; Wang, Xiaoqing; Zhu, Minya; Shi, Hongyu; Gui, Yu; Wang, Huishan; Zhou, Xin; Liao, Lisheng; Zhang, Lian‐Hui

doi:10.1128/aem.00325-22

Cited by 16 publications

(10 citation statements)

References 67 publications

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“…In addition, we observed a significant upregulation for the efpH gene, a virulence regulatory gene homologous to efpR [9], but exclusively in clones evolved in tomato Marmande and in cabbage. We also observed significant variations in the expression pattern of genes involved in quorum-sensingdependent virulence signaling pathways such as solI/solR [10] in 14 clones; rasI/rasR [11] in 24 clones; as well as the lectin encoding genes lecM and rsl2 [12] in 21 clones.…”

Section: Resultsmentioning

confidence: 85%

“…In addition, we observed a significant up-regulation for the efpH gene, a virulence regulatory gene homologous to efpR [9], but exclusively in clones evolved in tomato Marmande and in cabbage. We also observed significant variations in the expression pattern of genes involved in quorum-sensing-dependent virulence signaling pathways such as solI/solR [10] in 14 clones; rasI / rasR [11] in 24 clones; as well as the lectin encoding genes lecM and rsl2 [12] in 21 clones. Genes involved in the denitrification pathway such as narJ/narK1 and nosZ [13] were exclusively up-regulated in the tomato Marmande and cabbage clones and nasF , a nitrate transporter protein, was found up-regulated in 23 clones.…”

Section: Resultsmentioning

confidence: 99%

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Transcriptomic profiling reveals host-specific evolutionary pathways promoting enhanced fitness in the plant pathogenRalstonia pseudosolanacearum

Gopalan-Nair

Jardinaud

Genin

et al. 2023

Preprint

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The impact of host diversity on the genotypic and phenotypic evolution of broad-spectrum pathogens is a remaining issue. Here, we used populations of the plant pathogen Ralstonia pseudosolanacearum that were experimentally evolved on five types of host plants, either belonging to different botanical families or differing in their susceptibility or resistance to the pathogen. We investigated whether changes in transcriptomic profiles dissociated from genetic changes could occur during the process of host adaptation, and whether transcriptomic reprogramming was dependent on host type. Genomic and transcriptomic variations were established for 31 evolved clones that showed a better fitness in their experimental host than the ancestral clone. Few genomic polymorphisms were detected in these clones, but significant transcriptomic variations were observed, with a high number of differentially expressed genes (DEGs). In a very clear way, a group of genes belonging to the network of regulation of the bacterial virulence such as efpR, efpH or hrpB, among others, were deregulated in several independent evolutionary lineages and appeared to play a key role in the transcriptomic rewiring observed in evolved clones. A double hierarchical clustering based on the 400 top DEGs for each clone revealed two major patterns of gene deregulation that depend on host genotype, but not on host susceptibility or resistance to the pathogen. This work therefore highlights the existence of two major evolutionary paths that result in a significant reorganization of gene expression during adaptive evolution and underscore clusters of co-regulated genes associated to bacterial adaptation on different host lines.

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Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Transcriptomic profiling reveals host-specific evolutionary pathways promoting enhanced fitness in the plant pathogenRalstonia pseudosolanacearum

Gopalan-Nair

Jardinaud

Genin

et al. 2023

Preprint

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“…A recent study showed that the novel RasI/R quorum sensing system regulates phylotype I strain EP1 virulence. When the signal molecule N-(3-hydroxydodecanoyl)-homoserine lactone synthetic gene rasI was knocked out, the mutant lost virulence toward eggplants ( Yan et al, 2022 ). The metabolites of NRPS, NRPS-like, and T1PKS types were also only found in plant pathogenic Ralstonia , except for R. pickettii K-288 T , which only contained a T1PKS gene cluster.…”

Section: Resultsmentioning

confidence: 99%

Proposal to classify Ralstonia solanacearum phylotype I strains as Ralstonia nicotianae sp. nov., and a genomic comparison between members of the genus Ralstonia

Liu

Zhang

et al. 2023

Front. Microbiol.

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A Gram-negative, aerobic, rod-shaped, motile bacterium with multi-flagella, strain RST, was isolated from bacterial wilt of tobacco in Yuxi city of Yunnan province, China. The strain contains the major fatty acids of C16:0, summed feature 3 (C16:1ω7c and/or C16:1ω6c), and summed feature 8 (C18:1ω7c and/or C18:1ω6c). The polar lipid profile of strain RST consists of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and unidentified aminophospholipid. Strain RST contains ubiquinones Q-7 and Q-8. 16S rRNA gene sequence (1,407 bp) analysis showed that strain RST is closely related to members of the genus Ralstonia and shares the highest sequence identities with R. pseudosolanacearum LMG 9673T (99.50%), R. syzygii subsp. indonesiensis LMG 27703T (99.50%), R. solanacearum LMG 2299T (99.28%), and R. syzygii subsp. celebesensis LMG 27706T (99.21%). The 16S rRNA gene sequence identities between strain RST and other members of the genus Ralstonia were below 98.00%. Genome sequencing yielded a genome size of 5.61 Mbp and a G + C content of 67.1 mol%. The genomic comparison showed average nucleotide identity (ANIb) values between strain RST and R. pseudosolanacearum LMG 9673T, R. solanacearum LMG 2299T, and R. syzygii subsp. indonesiensis UQRS 627T of 95.23, 89.43, and 91.41%, respectively, and the corresponding digital DNA–DNA hybridization (dDDH) values (yielded by formula 2) were 66.20, 44.80, and 47.50%, respectively. In addition, strains belonging to R. solanacearum phylotype I shared both ANIb and dDDH with strain RST above the species cut-off values of 96 and 70%, respectively. The ANIb and dDDH values between the genome sequences from 12 strains of R. solanacearum phylotype III (Current R. pseudosolanacearum) and those of strain RST were below the species cut-off values. Based on these data, we concluded that strains of phylotype I, including RST, represent a novel species of the genus Ralstonia, for which the name Ralstonia nicotianae sp. nov. is proposed. The type strain of Ralstonia nicotianae sp. nov. is RST (=GDMCC 1.3533T = JCM 35814T).

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“…In addition, we observed a significant upregulation for the efpH gene, a virulence regulatory gene homologous to efpR [ 18 ], but exclusively in clones evolved in tomato Marmande and in cabbage. We also observed significant variations in the expression pattern of genes involved in quorum-sensing-dependent virulence signalling pathways such as solI/solR [ 19 ] in 14 clones; rasI / rasR [ 20 ] in 24 clones; as well as the lectin encoding genes lecM and rsl2 [ 21 ] in 21 clones. Genes involved in the denitrification pathway such as narJ/narK1 and nosZ [ 22 ] were exclusively upregulated in the tomato Marmande and cabbage clones and nasF , a nitrate transporter protein, was found to be upregulated in 23 clones.…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic profiling reveals host-specific evolutionary pathways promoting enhanced fitness in the plant pathogen Ralstonia pseudosolanacearum

Gopalan-Nair,

Jardinaud,

Legrand

et al. 2023

Microbial Genomics

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The impact of host diversity on the genotypic and phenotypic evolution of broad-spectrum pathogens is an open issue. Here, we used populations of the plant pathogen Ralstonia pseudosolanacearum that were experimentally evolved on five types of host plants, either belonging to different botanical families or differing in their susceptibility or resistance to the pathogen. We investigated whether changes in transcriptomic profiles, associated with or independent of genetic changes, could occur during the process of host adaptation, and whether transcriptomic reprogramming was dependent on host type. Genomic and transcriptomic variations were established for 31 evolved clones that showed better fitness in their experimental host than the ancestral clone. Few genomic polymorphisms were detected in these clones, but significant transcriptomic variations were observed, with a large number of differentially expressed genes (DEGs). In a very clear way, a group of genes belonging to the network of regulation of the bacterial virulence such as efpR, efpH or hrpB, among others, were deregulated in several independent evolutionary lineages and appeared to play a key role in the transcriptomic rewiring observed in evolved clones. A double hierarchical clustering based on the 400 top DEGs for each clone revealed 2 major patterns of gene deregulation that depend on host genotype, but not on host susceptibility or resistance to the pathogen. This work therefore highlights the existence of two major evolutionary paths that result in a significant reorganization of gene expression during adaptive evolution and underscore clusters of co-regulated genes associated with bacterial adaptation on different host lines.

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RasI/R Quorum Sensing System Controls the Virulence of Ralstonia solanacearum Strain EP1

Abstract: Quorum sensing (QS) is a key regulator of virulence factors in many plant-pathogenic bacteria. Previous studies unveiled two QS systems (i.e., PhcBSR and SolI/R) in several R. solanacearum strains.

Cited by 16 publications

References 67 publications

Transcriptomic profiling reveals host-specific evolutionary pathways promoting enhanced fitness in the plant pathogenRalstonia pseudosolanacearum

Transcriptomic profiling reveals host-specific evolutionary pathways promoting enhanced fitness in the plant pathogenRalstonia pseudosolanacearum

Proposal to classify Ralstonia solanacearum phylotype I strains as Ralstonia nicotianae sp. nov., and a genomic comparison between members of the genus Ralstonia

Transcriptomic profiling reveals host-specific evolutionary pathways promoting enhanced fitness in the plant pathogen Ralstonia pseudosolanacearum

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