Rat astrocytes are more supportive for mouse OPC self‐renewal than mouse astrocytes in culture

Cheng, Xiaowen; Xie, Binghua; Qi, Jiajun; Zhao, Xiaofeng; Zhang, Zunyi; Qiu, Mengsheng; Yang, Junlin

doi:10.1002/dneu.22476

Cited by 4 publications

(3 citation statements)

References 39 publications

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“…The directed differentiation of iOPCs derived from Sox10-GFP virus-induced transdifferentiation into mature oligodendrocytes was performed as previously described (Cheng et al 2017 ). Briefly, iOPCs were cultured in PDL/Laminin-coated chamber slides (Millipore, Bedford, USA) with glial basal media supplemented with 30 ng/ml Triiodothyronine (T3) (Sigma-Aldrich, St. Louis, USA) for 3 days, then examined for MBP antigen expression by immunostaining.…”

Section: Methodsmentioning

confidence: 99%

EGF signaling promotes the lineage conversion of astrocytes into oligodendrocytes

Liu

et al. 2022

Mol Med

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Background The conversion of astrocytes activated by nerve injuries to oligodendrocytes is not only beneficial to axonal remyelination, but also helpful for reversal of glial scar. Recent studies have shown that pathological niche promoted the Sox10-mediated astrocytic transdifferentiation to oligodendrocytes. The extracellular factors underlying the cell fate switching are not known. Methods Astrocytes were obtained from mouse spinal cord dissociation culture and purified by differential adherent properties. The lineage conversion of astrocytes into oligodendrocyte lineage cells was carried out by Sox10-expressing virus infection both in vitro and in vivo, meanwhile, epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) inhibitor Gefitinib were adopted to investigate the function of EGF signaling in this fate transition process. Pharmacological inhibition analyses were performed to examine the pathway connecting the EGF with the expression of oligodendrogenic genes and cell fate transdifferentiation. Results EGF treatment facilitated the Sox10-induced transformation of astrocytes to O4+ induced oligodendrocyte precursor cells (iOPCs) in vitro. The transdifferentiation of astrocytes to iOPCs went through two distinct but interconnected processes: (1) dedifferentiation of astrocytes to astrocyte precursor cells (APCs); (2) transformation of APCs to iOPCs, EGF signaling was involved in both processes. And EGF triggered astrocytes to express oligodendrogenic genes Olig1 and Olig2 by activating extracellular signal-regulated kinase 1 and 2 (Erk1/2) pathway. In addition, we discovered that EGF can enhance astrocyte transdifferentiation in injured spinal cord tissues. Conclusions These findings provide strong evidence that EGF facilitates the transdifferentiation of astrocytes to oligodendrocytes, and suggest that targeting the EGF-EGFR-Erk1/2 signaling axis may represent a novel therapeutic strategy for myelin repair in injured central nervous system (CNS) tissues.

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Section: Methodsmentioning

confidence: 99%

EGF signaling promotes the lineage conversion of astrocytes into oligodendrocytes

Liu

et al. 2022

Mol Med

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“…Immunocytochemical analysis was carried out as previously described (Cheng et al, 2017). Antibodies used include anti-mouse A2B5 IgM, anti-BrdU IgM, O4 IgM (1:1 dilution in DPBS + 10% goat serum), anti-mouse Olig2 (1:1000, Millipore), anti-mouse MBP (1:500, Abcam), anti- rabbit EGFR (1:200, Abcam), anti-mouse GFAP (1:300, Chemicon), anti-rabbit Nestin (1:2000, Covance), and anti-rabbit neurofilament (1:100, Sigma).…”

Section: Methodsmentioning

confidence: 99%

EGF Enhances Oligodendrogenesis from Glial Progenitor Cells

Yang

Cheng

et al. 2017

Front. Mol. Neurosci.

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Emerging evidence indicates that epidermal growth factor (EGF) signaling plays a positive role in myelin development and repair, but little is known about its biological effects on the early generation and differentiation of oligodendrocyte (OL) lineage cells. In this study, we investigated the role of EGF in early OL development with isolated glial restricted precursor (GRP) cells. It was found that EGF collaborated with Platelet Derived Growth Factor-AA (PDGFaa) to promote the survival and self-renewal of GRP cells, but predisposed GRP cells to develop into O4− early-stage oligodendrocyte precursor cells (OPCs) in the absence of or PDGFaa. In OPCs, EGF synergized with PDGFaa to maintain their O4 negative antigenic phenotype. Upon PDGFaa withdrawal, EGF promoted the terminal differentiation of OPCs by reducing apoptosis and increasing the number of mature OLs. Together, these data revealed that EGF is an important mitogen to enhance oligodendroglial development.

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“…The directed differentiation of iOPCs into mature oligodendrocytes was performed as previously described [17]. Brie y, iOPCs were cultured in PDL/Laminin-coated chamber slides (Millipore, CA) with glial basal media supplemented with 30 ng/ml Triiodothyronine (T3) for 3 days, then examined for MBP antigen expression by immunostaining.…”

Section: Iopc Differentiationmentioning

confidence: 99%

EGF signaling promotes the lineage conversion of astrocytes into oligodendroglias

Liu

Xie

et al. 2021

Preprint

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Background The conversion of astrocytes activated by nerve injuries to oligodendrocytes is not only beneficial to axonal remyelination, but also helpful for reversal of glial scar. Recent studies have shown that Sox10 transcription factor can achieve this transdifferentiation process in collaboration with some unknown factors in the pathological microenvironment. The extracellular factors underlying the cell fate switching are not known. Methods Astrocytes were obtained from mouse cortical dissociation culture and purified by differential adherent properties. The lineage conversion of astrocytes into oligodendrocyte lineage cells was carried out by Sox10-expressing virus infection both in vitro and in vivo, meanwhile, epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) inhibitor Gefitinib were adopted to investigate the function of EGF signaling in this fate transition process. Pharmacological inhibition analyses were performed to examine the pathway connecting the EGF with the expression of oligodendrogenic genes and cell fate transdifferentiation. Results EGF treatment facilitated the Sox10-induced transformation of astrocytes to O4+ induced oligodendrocyte precursor cells (iOPCs) in vitro. The transdifferentiation of astrocytes to iOPCs went through two distinct but interconnected processes: (1) dedifferentiation of astrocytes to astrocyte precursor cells (APCs); (2) transformation of APCs to iOPCs, EGF signaling was involved in both processes. And EGF triggered astrocytes to express oligodendrogenic genes Olig1 and Olig2 by activating extracellular signal-regulated kinase 1 and 2 (Erk1/2) pathway. In addition, we discovered that EGF can enhance astrocyte transdifferentiation in injured spinal cord tissues. Conclusions These findings provide strong evidence that EGF facilitates the transdifferentiation of astrocytes to oligodendroglias, and suggest that targeting the EGF-EGFR-Erk1/2 signaling axis may represent a novel therapeutic strategy for myelin repair in injured central nervous system (CNS) tissues.

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Rat astrocytes are more supportive for mouse OPC self‐renewal than mouse astrocytes in culture

Cited by 4 publications

References 39 publications

EGF signaling promotes the lineage conversion of astrocytes into oligodendrocytes

EGF signaling promotes the lineage conversion of astrocytes into oligodendrocytes

EGF Enhances Oligodendrogenesis from Glial Progenitor Cells

EGF signaling promotes the lineage conversion of astrocytes into oligodendroglias

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