Rat genotype influences quantitative and qualitative aspects of xenogeneic immune response to pig blood mononuclear cells

Abstract

Recipient genotype had significant and broad effects on IR to porcine BMC and may influence xenograft rejection and xenotolerance induction. Moreover, this study suggests that caution should prevail when interpreting data derived from a single inbred strain, particularly given that humans, the target species, are genetically diverse.

Analysis of gene expression patterns by microarray hybridization in blood mononuclear cells of SLA-DRB1 defined Canadian Yorkshire pigs

“…Therefore, SLA-defined pigs constitute an invaluable resource to study immune response, disease resistance and production traits, as well as an important large animal model for biomedical research [ 5 , 6 ]. Three lines of commercial Yorkshire pigs with defined SLA-DRB1 genotypes were produced at the University of Guelph for xenotransplantation and immune response research [ 7 , 8 ]. The aims of this study were to characterize the SLA-DRB1 alleles in these three pig lines and explore differential transcriptional activity between the three groups using heterologous (bovine probes – porcine targets) cDNA microarray and qPCR.…”