Rat liver lysosomal and mitochondrial activities are modified by anabolic-androgenic steroids

Molano, F; Saborido, Ana; Delgado, Jerónimo; Moran, Mary C.; Megías, Alicia

doi:10.1097/00005768-199902000-00007

Cited by 36 publications

(38 citation statements)

References 33 publications

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“…These results suggest that BGLU levels could also be influenced by the male hormone. A similar conclusion was reached by Molano et al (24) in a study in which higher BGLU levels were observed in liver homogenates from androgenic steroid-treated rats than in liver homogenates from untreated animals.…”

Section: Discussionsupporting

confidence: 85%

Serum hexosaminidase and ß-glucuronidase activities in infants: effects of age and sex

Mabe

Beck

2003

Braz J Med Biol Res

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We investigated the effect of age and sex on the serum activity of hexosaminidase (HEX) and ß-glucuronidase (BGLU) in 275 normal term infants aged 12 h to 12 months. Up to six weeks of life, HEX was significantly higher in boys (P£0.023). During the age period of 1-26 weeks, BGLU was also higher in boys, but differences were significant only at 2-6 and 7-15 weeks (P£0.016). The developmental pattern of HEX and BGLU was sex dependent. HEX activity increased in both sexes from 4-7 days of life, reaching a maximum of 1.4-fold the birth value at 2-6 weeks of age in boys (P<0.001) and a maximum of 1.6-fold at 7-15 weeks in girls (P<0.001). HEX activity gradually decreased thereafter, reaching significantly lower levels at 27-53 weeks than during the first three days of life in boys (P = 0.002) and the same level of this age interval in girls. BGLU increased in both sexes from 4-7 days of age, showing a maximum increase at 7-15 weeks (3.3-fold in boys and 2.9-fold in girls, both P<0.001). Then BGLU decreased in boys to a value similar to that observed at 4-7 days of age. In girls, BGLU remained elevated until the end of the first year of life. These results indicate a variation of HEX and BGLU activities during the first year of life and a sex influence on their developmental pattern. This observation should be considered in the diagnosis of GM 2 gangliosidosis and mucopolysaccharidosis type VII.

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Section: Discussionsupporting

confidence: 85%

Serum hexosaminidase and ß-glucuronidase activities in infants: effects of age and sex

Mabe

Beck

2003

Braz J Med Biol Res

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“…This observation is consistent with previously reported results [7,21]. On the contrary, some studies reported that the supraphysiologic doses of AAS are associated with an increase in the plasma levels of these parameters [29][30][31].…”

Section: Alt (Iu/l) Ast (Iu/l) Alp (Iu/l)supporting

confidence: 93%

“…In addition, the conventional liver function tests do not always reflect liver abnormalities particularly at the initial stages. In this regard, previous studies indicate that prolonged AAS administration causes inflammatory or degenerative lesions in centrilobular hepatocytes, ultrastructural alterations in the canaliculi and degenerative changes in mitochondria and lysosomes without modifying classical serum indicators of hepatic function [6,7,21].…”

Section: Alt (Iu/l) Ast (Iu/l) Alp (Iu/l)mentioning

confidence: 96%

“…The elevation of SOD, GPx and GR levels may be attributed to hepatic dysfunction of mitochondrial respiratory chain complexes and mono-oxygenase systems in response to Su administration [7,21], it would be possible that these alterations were accompanied by an increased ROS production. Excessive ROS formation could lead to oxidative stress and cell damage [22].…”

Section: Alt (Iu/l) Ast (Iu/l) Alp (Iu/l)mentioning

confidence: 99%

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Effects of Resistance Training Accompanied by Sustanon Abuse on Hepatotoxicity in Rats with and without Training Experience

Rahmati¹,

Arazi²,

Ghafoori³

2017

Sports Nutr Ther

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Objectives: Abuse of anabolic androgenic steroids (AAS) has increased. Previous studies have shown that resistance training (RT) is the main exercise modality practiced by AAS abusers and people often use them in different training situations. Thus propose of this study was to evaluate the hepatotoxic effects of sustanon (Su) as an example of anabolic androgenic steroids in male rats with and without RT experience.Methods: Rats were divided into seven groups: control; Su -untreated sedentary rats (non-Su/ Sed); Sutreated sedentary rats (Su/Sed); Su -untreated trained rats (non-Su/Tr); Su -treated trained rats (Su/Tr); Suuntreated experience trained rats (non-Su/XTr); Su -treated experience trained rats (Su/XTr). Su -treated groups received sustanon 10 mg/kg intramuscularly once a week for 8 weeks. In the Tr and XTr groups, the animals climbed a 1.1 m vertical ladder, 3 days per week for 8 and 12 week, respectively. Results:After Su -treatment, the mean values of serum parameters related to hepatic function were within normal ranges. Superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GR) activities were higher (P<0.05) in the liver of Su treatment groups. Tr and XTr did not change any of the above parameters. Conclusion:The present findings suggest that the injection of Su during 8 weeks, either with or without RT up-regulation of enzymatic antioxidant activities. Moreover, these data demonstrated that liver function tests do not always reflect liver abnormalities particularly at the initial stages.

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“…These results are also consistent with Welder et al (1995) who cited that AAS have toxic effects in primary rat hepatic cultures. Controversially, Molano et al (1999) reported that prolonged administration of anabolic steroid stanozolol had no significant effect on classical serum markers of liver function. The increment in serum AST and ALT activities may be attributed to the release of these enzymes from the cystol of liver cells into the blood circulation as a result of hepatocytes damage (Navarro et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Boldenone-induced apoptotic, structural, and functional alterations in the liver of rabbits

2015

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Boldenone undecylenate (BOL) is an anabolic androgenic steroid used in livestock to improve growth and food conversion. This study investigated the actions of BOL on structure and functions of rabbit liver as well as the effects of its withdrawal. Eighteen mature male New Zealand rabbits were divided into 2 groups: Control group (n=6) were injected with 0.25 mL corn oil/kg body weight (BW), while BOL group (n=12) received 3 intramuscular injections, 2 wk apart, of BOL (4.5 mg/kg BW). Animals were scarified 1 d after last injection except for 6 rabbits from BOL group that served as the BOL-withdrawal group (4 wk after the 3 rd injection). Intramuscular injection of BOL increased (P<0.05) malondialdehyde (MDA) level, but markedly lowered activities of superoxide dismutase (SOD) and catalase (CAT) and reduced glutathione (GSH) concentration compared to both control and BOL-withdrawal groups. Treatment with BOL significantly (P<0.05) increased serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) compared to the control group. BOL injection caused different histopathological alterations and apoptosis in liver, but these changes were less evident in the BOL-withdrawal group. Expression of p53 and tumour necrosis factor-α (TNF-α) genes was up regulated in BOL compared to control group, while the expressions of p53 and TNF-α were down regulated in BOL-withdrawal group in comparison with BOL group. In conclusion, BOL injection induced structural and functional changes in the liver of rabbits, increasing oxidative stress and mediators of apoptosis such as ROS, p53 and TNF-α. All these parameters returned to near the control values after withdrawal.

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Rat liver lysosomal and mitochondrial activities are modified by anabolic-androgenic steroids

Cited by 36 publications

References 33 publications

Serum hexosaminidase and ß-glucuronidase activities in infants: effects of age and sex

Serum hexosaminidase and ß-glucuronidase activities in infants: effects of age and sex

Effects of Resistance Training Accompanied by Sustanon Abuse on Hepatotoxicity in Rats with and without Training Experience

Boldenone-induced apoptotic, structural, and functional alterations in the liver of rabbits

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