Rat lung decellularization using chemical detergents for lung tissue engineering

Tebyanian, Hamid; Karami, Ali; Motavallian, Ebrahim; Samadikuchaksaraei, Alí; Arjmand, Babak; Nourani, M R

doi:10.1080/10520295.2018.1544376

Cited by 25 publications

(9 citation statements)

References 25 publications

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“…As such, several protocols have been published which indicate that the lungs can be decellularized by chemical, enzymatic, physical, and combinative methods. 235 , 236 Most commonly, lung tissue is decellularized by perfusion through the airways, vasculature, or both, with combinations of detergents such as SDC and Triton X-100, or individually by low concentrations of SDS or CHAPS. Protocols have utilized different concentrations for each reagent, exposure times to reagents, routes for perfusion, and order of reagent administration, each with differing levels of success.…”

Section: Recent Novel Approaches To Decellularization By Tissue Typementioning

confidence: 99%

“…Protocols have utilized different concentrations for each reagent, exposure times to reagents, routes for perfusion, and order of reagent administration, each with differing levels of success. Tebyanian et al 236 found that a detergent-based approach using 2 mM CHAPS and 0.1% SDS for 24 h maintained the microarchitecture of rat lung better than 2 mM CHAPS and 0.5% Triton X-100 for 96 h. It is important to note that a donor tissue source must be taken into consideration when choosing a means of introducing decellularizing agents into the tissue, as lung from species of differing sizes cannot be decellularized under the same conditions. For instance, pressure-based perfusion of decellularizing agents into the tissue should be avoided for lungs sourced from larger donors, such as human or pig, but are acceptable for lungs sourced from smaller donors such as mouse or rat.…”

Section: Recent Novel Approaches To Decellularization By Tissue Typementioning

confidence: 99%

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Decellularization for the retention of tissue niches

Moffat

Jin

2022

J Tissue Eng

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Decellularization of natural tissues to produce extracellular matrix is a promising method for three-dimensional scaffolding and for understanding microenvironment of the tissue of interest. Due to the lack of a universal standard protocol for tissue decellularization, recent investigations seek to develop novel methods for whole or partial organ decellularization capable of supporting cell differentiation and implantation towards appropriate tissue regeneration. This review provides a comprehensive and updated perspective on the most recent advances in decellularization strategies for a variety of organs and tissues, highlighting techniques of chemical, physical, biological, enzymatic, or combinative-based methods to remove cellular contents from tissues. In addition, the review presents modernized approaches for improving standard decellularization protocols for numerous organ types.

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Section: Recent Novel Approaches To Decellularization By Tissue Typementioning

confidence: 99%

Section: Recent Novel Approaches To Decellularization By Tissue Typementioning

confidence: 99%

Decellularization for the retention of tissue niches

Moffat

Jin

2022

J Tissue Eng

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“…Samples were dehydrated in a serial dilution of ethanol, embedded in paraffin, and sectioned to 5.0 μm thicknesses using a Leica microtome (Leica Microsystems, Wetziar, Germany). Hematoxylin and Eosin (H&E) staining (Figure 5), Safranin O/Fast Green staining, and Immunohistochemical staining of collagen type II and type I (Figure 6), were performed for all sections and inspected using a light microscope [32][33][34][35][36] (Olympus, Shinjuku, Tokyo). In this study, scores were determined using histological sections from both the defect area and the adjacent natural cartilage to provide a rational judgment about the healing pattern and tissue integration according to the modified O'Driscoll scoring system (Table S1).…”

Section: Histopathological Studiesmentioning

confidence: 99%

Comparison of engineered cartilage based on BMSCs and chondrocytes seeded on PVA‐PPU scaffold in a sheep model

et al. 2022

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In this study, polyvinyl alcohol hydrogel chains were crosslinked by polyurethane in order to synthesize a suitable substrate for cartilage lesions. The substrate was fully characterized, and in vitro and in vivo investigations were conducted based on a sheep model. In vitro tests were performed based on the chondrocyte cells with the Alcian Blue and safranin O staining in order to prove the presence of proteoglycan on the surface of the synthesized substrate, which has been secreted by cultures of chondrocytes. Furthermore, the expression of collagen type I, collagen type II, aggrecan, and Sox9 was presented in the chondrocyte cultures on the synthesized substrate through RT-PCR. In addition, the H&E analysis and other related tests demonstrated the formation of neocartilage tissue in a sheep model. The results were found to be promising for cartilage tissue engineering and verified that the isolated chondrocyte cultures on the synthesized substrate retain their original composition. K E Y W O R D S cartilage tissue engineering, polyvinyl alcohol-polyurethane composite, sheep model 1 | INTRODUCTION Joint problems such as traumatic lesions of articular hyaline cartilages in athletes and people have created a major challenge in the general public health. Cartilage damages in both severe injuries and osteoarthritis are irreversible processes with no approved medical product for complete repair of cartilage dysfunction. 1 Articular cartilage has a limited capacity of self-repair due to its low cellularity and avascular matrix. A Masoud Taghizadehjahed and Asma Sepahdar contributed equally to this work.

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“…Triton X‐100 has also been used in combination with SDS to assist its wash during the procedure, which resulted in efficient cell removal and ECM preservation . Zwitterionic detergents such as 3‐[(3‐cholamidopropyl)dimethylammonio]‐1‐propanesulfonate (CHAPS) exhibit nondenaturing properties and were shown highly beneficial in maintaining ECM integrity of thin tissues like the lungs . Another type of chemical agents are alcohols, which dehydrate and lyse cells and were shown advantageous for lipid removal .…”

Section: Ecm Decellularization Techniquesmentioning

confidence: 99%

Processed Tissue–Derived Extracellular Matrices: Tailored Platforms Empowering Diverse Therapeutic Applications

Krishtul

Baruch

Machluf

2019

Adv Funct Materials

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Tissue-derived decellularized extracellular matrices (dECM) have gradually become the gold standard of scaffolds for tissue engineering, owing to their close mirroring of the intricate composition, architecture, and topology of the native extracellular matrix (ECM). Intriguingly, further manipulation of these acellular tissues through various processing techniques has been demonstrated to be an effective strategy to control their characteristics and impart them with ample valuable new traits, thereby expanding their applicability to a significantly wider spectrum of research and translational applications. Herein, state-of-the-art processed dECM platforms and their potential applications are focused on. The ECM characteristics that make it so appealing for tissue engineering are presented, followed by a concise discussion on the main considerations for choosing a dECM source for such applications. The key methodologies for dECM processing, including hydrogel production, bioprinting, electrospinning, and production of porous scaffolds, microcarriers, and microcapsules, as well as their inherent advantages and challenges, are introduced. To demonstrate the use of processed dECM platforms for tissue engineering, selected in vivo and in vitro applications recently developed utilizing these platforms are highlighted. Finally, concluding remarks and a prospective outlook for future developments and improvements in the field of processed dECM-based devices are given.

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Rat lung decellularization using chemical detergents for lung tissue engineering

Cited by 25 publications

References 25 publications

Decellularization for the retention of tissue niches

Decellularization for the retention of tissue niches

Comparison of engineered cartilage based on BMSCs and chondrocytes seeded on PVA‐PPU scaffold in a sheep model

Processed Tissue–Derived Extracellular Matrices: Tailored Platforms Empowering Diverse Therapeutic Applications

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