Rat renal arcade segment expresses vasopressin-regulated water channel and vasopressin V2 receptor.

Kishore, Bellamkonda; Mandon, B.; Oza, Narendra B.; DiGiovanni, Susan R.; Coleman, Richard A.; Ostrowski, Nancy L.; Wade, James B.; Knepper, Mark A.

doi:10.1172/jci118731

Cited by 64 publications

(48 citation statements)

References 34 publications

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“…Vasopressin receptors and AQP2 have also been identified in CNT in rat, consistent with emerging evidence of a role for this segment in water balance (25,33). The present studies do not exclude the presence of vasopressin receptors in CNT in mouse and human kidney; however, given the gradual transition from DCT to CNT and from CNT to CCD in these species (11), with overlap of Na ϩ -Cl Ϫ cotransporter, ENaC, and AQP2 staining along the distal convolution, precise identification of this segment is problematic.…”

Section: Discussionsupporting

confidence: 69%

Axial heterogeneity of vasopressin-receptor subtypes along the human and mouse collecting duct

Carmosino¹,

Brooks²,

Cai³

et al. 2007

American Journal of Physiology-Renal Physiology

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Vasopressin and vasopressin antagonists are finding expanded use in mouse models of disease and in clinical medicine. To provide further insight into the physiological role of V1a and V2 vasopressin receptors in the human and mouse kidney, intrarenal localization of the receptors mRNA was determined by in situ hybridization. V2-receptor mRNA was predominantly expressed in the medulla, whereas mRNA for V1a receptors predominated in the cortex. The segmental localization of vasopressinreceptor mRNAs was determined using simultaneous in situ hybridization and immunohistochemistry for segment-specific markers, including aquaporin-2, Dolichos biflorus agglutinin, epithelial Na channels, Tamm Horsfall glycoprotein, and thiazide-sensitive Na ϩ -Cl Ϫ cotransporter. Notably, V1a receptor expression was exclusively expressed in V-ATPase/anion exchanger-1-labeled alpha-intercalated cells of the medullary collecting duct in both mouse and human kidney. In cortical collecting ducts, V1a mRNA was more widespread and detected in both principal and intercalated cells. V2-receptor mRNA is diffusely expressed along the collecting ducts in both mouse and human kidney, with higher expression levels in the medulla. These results demonstrate heterogenous axial expression of both V1a and V2 vasopressin receptors along the human and mouse collecting duct. The restricted expression of V1a-receptor mRNA in intercalated cells suggests a role for this receptor in acid-base balance. These findings further suggest distinct regulation of renal transport function by AVP through V1a and V2 receptors in the cortex vs. the medulla. in situ hybridization; vasopressin receptors; intercalated cell ARGININE VASOPRESSIN (AVP) is the key hormone responsible for producing a concentrated urine and is secreted in high concentrations during antidiuresis. At least two different vasopressin

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Section: Discussionsupporting

confidence: 69%

Axial heterogeneity of vasopressin-receptor subtypes along the human and mouse collecting duct

Carmosino¹,

Brooks²,

Cai³

et al. 2007

American Journal of Physiology-Renal Physiology

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“…Comparable results have been reported in the kidneys of rats, mice, rabbits, dogs, monkeys, horses, and mini pigs Kishore et al, 1996;Loffing et al, 2000;Rojek et al, 2006;Eskild-Jedsen et al, 2007;Floyd et al, 2007;Bauchet et al, 2011). To our knowledge, the present study is the first report of AQP2 localization and expression in the kidneys of Sus scrofa.…”

Section: Discussionsupporting

confidence: 77%

Immunohistochemical identification of aquaporin 2 in the kidneys of wild boars (Sus scrofa)

Michałek¹,

Czerniawska-Piątkowska²,

Grabowska³

et al. 2015

Turk J Biol

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The first aquaporin discovered, AQP2, was found in 1993 by Fushimi and coworkers in rat renal collecting tubules (Fushimi et al., 1993). In subsequent years, the presence of this protein was also confirmed in human and mouse kidneys. In the different sections of renal tubules 7 additional isoforms of AQPs (AQP1, AQP3, AQP4, AQP6, AQP7, AQP8, and AQP11) have been identified, but AQP2 is of particular importance for the regulation of water balance according to body needs. It is this protein that is fused to the apical plasma membrane in response to stimulation by antidiuretic hormone vasopressin (AVP) in the collecting duct principal cells, thereby increasing membrane permeability to water. As a result, low amounts of concentrated urine are excreted (Noda and Sasaki, 2005).

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“…11,12 Aquaporin 2 (AQP2) is a water channel expressed along the CNT and CD. 13,14 Our data indicate that ␣ENaC expression within the CNT is important for sodium and potassium balance.…”

mentioning

confidence: 73%

Sodium and Potassium Balance Depends on αENaC Expression in Connecting Tubule

Christensen¹,

Perrier²,

Wang

et al. 2010

Journal of the American Society of Nephrology

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Mutations in ␣, ␤, or ␥ subunits of the epithelial sodium channel (ENaC) can downregulate ENaC activity and cause a severe salt-losing syndrome with hyperkalemia and metabolic acidosis, designated pseudohypoaldosteronism type 1 in humans. In contrast, mice with selective inactivation of ␣ENaC in the collecting duct (CD) maintain sodium and potassium balance, suggesting that the late distal convoluted tubule (DCT2) and/or the connecting tubule (CNT) participates in sodium homeostasis. To investigate the relative importance of ENaC-mediated sodium absorption in the CNT, we used Cre-lox technology to generate mice lacking ␣ENaC in the aquaporin 2-expressing CNT and CD. Western blot analysis of microdissected cortical CD (CCD) and CNT revealed absence of ␣ENaC in the CCD and weak ␣ENaC expression in the CNT. These mice exhibited a significantly higher urinary sodium excretion, a lower urine osmolality, and an increased urine volume compared with control mice. Furthermore, serum sodium was lower and potassium levels were higher in the genetically modified mice. With dietary sodium restriction, these mice experienced significant weight loss, increased urinary sodium excretion, and hyperkalemia. Plasma aldosterone levels were significantly elevated under both standard and sodium-restricted diets. In summary, ␣ENaC expression within the CNT/CD is crucial for sodium and potassium homeostasis and causes signs and symptoms of pseudohypoaldosteronism type 1 if missing.

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Rat renal arcade segment expresses vasopressin-regulated water channel and vasopressin V2 receptor.

Cited by 64 publications

References 34 publications

Axial heterogeneity of vasopressin-receptor subtypes along the human and mouse collecting duct

Axial heterogeneity of vasopressin-receptor subtypes along the human and mouse collecting duct

Immunohistochemical identification of aquaporin 2 in the kidneys of wild boars (Sus scrofa)

Sodium and Potassium Balance Depends on αENaC Expression in Connecting Tubule

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