Rat subthalamic stimulation: Evaluating stimulation-induced dyskinesias, choosing stimulation currents and evaluating the anti-akinetic effect in the cylinder test

Huotarinen, Antti; Leino, Sakari; Tuominen, Raimo K.; Laakso, Aki

doi:10.1016/j.mex.2019.10.012

Cited by 4 publications

(2 citation statements)

References 41 publications

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“…A cylinder test was performed to evaluate the motor function recovery of the forelimbs as previously described [ 17 , 18 ]. The rat was placed in a glass cylinder (50 cm in diameter, 70 cm in height).…”

Section: Methodsmentioning

confidence: 99%

Acetylglutamine facilitates motor recovery and alleviates neuropathic pain after brachial plexus root avulsion in rats

Wu,

Chen,

et al. 2023

J Transl Med

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Background Brachial plexus root avulsion (BPRA), a disabling peripheral nerve injury, induces substantial motoneuron death, motor axon degeneration and denervation of biceps muscles, leading to the loss of upper limb motor function. Acetylglutamine (N-acetyl-L-glutamine, NAG) has been proven to exert neuroprotective and anti-inflammatory effects on various disorders of the nervous system. Thus, the present study mainly focused on the influence of NAG on motor and sensory recovery after BPRA in rats and the underlying mechanisms. Methods Male adult Sprague Dawley (SD) rats were subjected to BPRA and reimplantation surgery and subsequently treated with NAG or saline. Behavioral tests were conducted to evaluate motor function recovery and the mechanical pain threshold of the affected forelimb. The morphological appearance of the spinal cord, musculocutaneous nerve, and biceps brachii was assessed by histological staining. Quantitative real-time PCR (qRT‒PCR) was used to measure the mRNA levels of remyelination and regeneration indicators in myocutaneous nerves. The protein levels of inflammatory and pyroptotic indicators in the spinal cord anterior horn were measured using Western blotting. Results NAG significantly accelerated the recovery of motor function in the injured forelimbs, enhanced motoneuronal survival in the anterior horn of the spinal cord, inhibited the expression of proinflammatory cytokines and pyroptosis pathway factors, facilitated axonal remyelination in the myocutaneous nerve and alleviated atrophy of the biceps brachii. Additionally, NAG attenuated neuropathic pain following BPRA. Conclusion NAG promotes functional motor recovery and alleviates neuropathic pain by enhancing motoneuronal survival and axonal remyelination and inhibiting the pyroptosis pathway after BPRA in rats, laying the foundation for the use of NAG as a novel treatment for BPRA.

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Section: Methodsmentioning

confidence: 99%

Acetylglutamine facilitates motor recovery and alleviates neuropathic pain after brachial plexus root avulsion in rats

Wu,

Chen,

et al. 2023

J Transl Med

View full text Add to dashboard Cite

show abstract

“…A Cylinder Test was performed to evaluate the motor function of the affected forelimbs as described in the literature [16,17]. The rat was placed in a glass cylinder (50 cm in diameter, 70 cm in height).…”

Section: Cylinder Testmentioning

confidence: 99%

Acetylglutamine facilitates motor recovery after brachial plexus root avulsion in ratsby promoting motoneuronal survival and axonal remyelination

Chen

et al. 2023

Preprint

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Background: Brachial plexus root avulsion (BPRA) is a disabling peripheral nerve injury that induces substantial death of motoneurons, degeneration of motor axons and de-innervation of biceps muscles, leading toloss of upper limb motor function. Acetylglutamine (N-acetyl-L-glutamine, NAG) has been proved to exert neuroprotective and anti-inflammatory effects in various disorders in the nervous system. Hence, the present study focused on the effect of NAG on motor recovery after BPRA in rats and the underlying mechanisms. Methods: Adult male Sprague Dawley rats were subject to BPRA and reimplantation surgery andsubsequently treated with NAG or saline. Behavioral tests were conducted to evaluate motor function recovery and mechanical pain threshold of the affected forelimb. The morphological appearance of the spinal cord, musculocutaneous nerve, and biceps brachii was assessed by histological staining. Quantitative real-time PCR was utilized to measure the mRNA levels of remyelination and regenerstion indicators on myocutaneous nerves. The protein levels of inflammatory and pyroptotic indicators in the anterior horn of the spinal cord were measured using Western blot analysis. Results: Our results indicated NAG could significantly accelerate recovery of motor function in the injured forelimbs, enhance motoneuronal survival in the anterior horn of the spinal cord, inhibit the expression of proinflammatory cytokines and pyroptosis pathway, facilitate axonal remyelination in the myocutaneous nerve and alleviate atrophy of biceps brachii. Additionally, NAG attenuated neuropathic pain following BPRA. Conclusion: NAG promotes functional motor recovery by enhancing motoneuronal survival and axonal remyelination and inhibiting the pyroptosis pathway after BPRA in rats, laying the foundation for NAG to be a novel strategy for BPRA treatment.

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Therapeutic Efficacy and Migration of Mesenchymal Stem Cells after Intracerebral Transplantation in Rats with Experimental Ischemic Stroke

et al. 2023

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Rat subthalamic stimulation: Evaluating stimulation-induced dyskinesias, choosing stimulation currents and evaluating the anti-akinetic effect in the cylinder test

Abstract: Graphical abstract

Cited by 4 publications

References 41 publications

Acetylglutamine facilitates motor recovery and alleviates neuropathic pain after brachial plexus root avulsion in rats

Acetylglutamine facilitates motor recovery and alleviates neuropathic pain after brachial plexus root avulsion in rats

Acetylglutamine facilitates motor recovery after brachial plexus root avulsion in ratsby promoting motoneuronal survival and axonal remyelination

Therapeutic Efficacy and Migration of Mesenchymal Stem Cells after Intracerebral Transplantation in Rats with Experimental Ischemic Stroke

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