Ratiometric Fluorescent Lateral Flow Immunoassay for Point‐of‐Care Testing of Acute Myocardial Infarction

Wang, Jing; Jiang, Chenxing; Jin, Jiening; Huang, Liang; Yu, Wenbo; Su, Bin; Hu, Jun

doi:10.1002/ange.202103458

Cited by 122 publications

(19 citation statements)

References 66 publications

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“…To achieve rapid on-site detection, we designed a smartphone-based immunochromatography reader and developed an analysis application that could rapidly quantify the results [ 21 , 22 ]. As shown in Figure 6 A, we used our smartphone camera to directly capture the fluorescent images of the strips using a small and portable reader.…”

Section: Resultsmentioning

confidence: 99%

Quantum Dot Nanobeads as Multicolor Labels for Simultaneous Multiplex Immunochromatographic Detection of Four Nitrofuran Metabolites in Aquatic Products

et al. 2022

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A multicolor immunochromatographic assay platform based on quantum dot nanobeads (QBs) for the rapid and simultaneous detection of nitrofuran metabolites in different aquatic products is documented. These metabolites include 3-amino-2-oxazolidinone (AOZ), 1-aminohydantoin (AHD), semicarbazide (SEM), and 3-amino-5-morpholino-methyl-1,3-oxazolidinone (AMOZ). QBs with emission colors of red, yellow, green, and orange were employed and functionalized with the corresponding antibodies to each analyte to develop a multicolor channel. The visual detection limits (cutoff values) of our method for AOZ, AHD, SEM, and AMOZ reached up to 50 ng/mL, which were 2, 20, 20, and 20 times lower than those of traditional colloidal gold test strips, respectively. The test strip is capable of detection within 10 min in real samples while still achieving good stability and specificity. These results demonstrate that the developed multicolor immunochromatographic assay platform is a promising technique for multiplex, highly sensitive, and on-site detection of nitrofuran metabolites.

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Section: Resultsmentioning

confidence: 99%

Quantum Dot Nanobeads as Multicolor Labels for Simultaneous Multiplex Immunochromatographic Detection of Four Nitrofuran Metabolites in Aquatic Products

et al. 2022

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“…For the purpose of enhancing visual assay capacity of conventional FL immunochromatographic assay, our group demonstrated a ratiometric fluorescent lateral flow immunoassay (RFLFIA) strip for detecting heart‐type fatty acid‐binding protein (H‐FABP) visually [84] . As shown in Figure 5d, the RFLFIA strip with two reversed signals was designed with an appropriate reporter and capture probes consisting of two kinds of QDs conjugated with two different types of H‐FABP antibodies.…”

Section: Visual Sensing Applicationsmentioning

confidence: 99%

Semiconductor/Carbon Quantum Dot‐based Hue Recognition Strategy for Point of Need Testing: A Review

Wang

2023

ChemistryOpen

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The requirement to establish novel methods for visual detection is attracting attention in many application fields of analytical chemistry, such as, healthcare, environment, agriculture, and food. The research around subjects like “point‐of‐need”, “hue recognition”, “paper‐based sensor”, “fluorescent sensor”, etc. has been always aimed at the opportunity to manufacture convenient and fast‐response devices to be used by non‐specialists. It is possible to achieve economic rationality and technical simplicity for optical sensing toward target analytes through introduction of fluorescent semiconductor/carbon quantum dot (QD) and paper‐based substrates. In this Review, the mechanisms of anthropic visual recognition and fluorescent visual assays, characteristics of semiconductor/carbon QDs and ratiometric fluorescence test paper, and strategies of semiconductor/carbon QD‐based hue recognition are described. We cover latest progress in the development and application of point‐of‐need sensors for visual detection, which is based on a semiconductor/carbon quantum dot‐based hue recognition strategy generated by ratiometric fluorescence technology.

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“…Fluorescence-based optical test strip readers sense and quantify fluorescent signals from immunoassay test strips in rapid and accurate detection of target analytes at the point of care and are used for environmental monitoring, food safety [1,2], and medical diagnosis [3][4][5][6][7][8]. These devices employ fluorescence technology in detecting specific substances in a biological sample.…”

Section: Introductionmentioning

confidence: 99%

Emerging Technologies for Fluorescence-Based Optical Test Strip Readers

AKSOY

DULDA

Ertas

2023

EJOSAT

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Fluorescence-based optical test strip readers are used to detect and quantify fluorescent signals from immunoassay test strips in medicine, especially for point-of-care applications. The design of optical systems including light sources and detection systems in these devices is not only indispensable but also the most critical part for specific detection applications. This study aims to provide detailed information about fluorescence-based optical test strip readers, existing and emerging technologies, and their contributions to the design of the device. The most commonly used technologies of light sources and detection systems have been discussed and compared for the ideal design. Arc and Xenon lamps may not be appropriate for portable and low-cost devices as they are larger and more costly when compared to LEDs and laser diodes. Photodiodes and CMOS detectors can be used for the design of low-cost, portable fluorescence-based optical test strip readers as they are cheaper and smaller in size when compared to CCDs and PMTs. Both light source and detector should be chosen according to the application priorities and spectral characteristics of the fluorescent molecule by integrating them with proper optical elements like filters, mirrors, etc. This study contributes to the people who are interested in the design of fluorescence-based optical test strip readers as it serves as a guideline for the optical test strip reader systems.

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Ratiometric Fluorescent Lateral Flow Immunoassay for Point‐of‐Care Testing of Acute Myocardial Infarction

Cited by 122 publications

References 66 publications

Quantum Dot Nanobeads as Multicolor Labels for Simultaneous Multiplex Immunochromatographic Detection of Four Nitrofuran Metabolites in Aquatic Products

Quantum Dot Nanobeads as Multicolor Labels for Simultaneous Multiplex Immunochromatographic Detection of Four Nitrofuran Metabolites in Aquatic Products

Semiconductor/Carbon Quantum Dot‐based Hue Recognition Strategy for Point of Need Testing: A Review

Emerging Technologies for Fluorescence-Based Optical Test Strip Readers

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