Rational molecular design for improving digestive enzyme resistance of beta-glucosidase from Trichoderma viride based on inhibition of bound state formation

Wang, Hao; Lin, Xiangna; Li, Shuang; Lin, Jianlin; Xie, Chen; Liu, Daling; Yao, Dongsheng

doi:10.1016/j.enzmictec.2019.109465

Cited by 10 publications

(6 citation statements)

References 48 publications

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“…A standard colorimetric assay was developed on the basis of previously reported methods with slight modifications (Liu and Zhu, 2015;Kornecki et al, 2020;Wang et al, 2020). In brief, 25 µL appropriately diluted fermentation broth (containing cells) was added to 75 µL of 5 mM pNPG.…”

Section: β-Glucosidase Activity Assaymentioning

confidence: 99%

Development of a New High-Cell Density Fermentation Strategy for Enhanced Production of a Fungus β-Glucosidase in Pichia pastoris

et al. 2020

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Section: β-Glucosidase Activity Assaymentioning

confidence: 99%

Development of a New High-Cell Density Fermentation Strategy for Enhanced Production of a Fungus β-Glucosidase in Pichia pastoris

et al. 2020

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“…Bioinformatics analysis is used to provide conserved regions of thermostable enzymes or active sites for mutation through semi-rational design ( Wang et al, 2018 ; Wang H. et al, 2020 ). A semi-rational design was done to alter the activity of a chitinase PpChi1 cloned from Paenibacillus pasadenensis CS0611.…”

Section: Strategies To Enhance Thermostability and Specific Activity Of Cellulase And Xylanase Of Thermophilesmentioning

confidence: 99%

Thermostable Cellulases / Xylanases From Thermophilic and Hyperthermophilic Microorganisms: Current Perspective

Ajeje¹,

Hu²,

Song³

et al. 2021

Front. Bioeng. Biotechnol.

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The bioconversion of lignocellulose into monosaccharides is critical for ensuring the continual manufacturing of biofuels and value-added bioproducts. Enzymatic degradation, which has a high yield, low energy consumption, and enhanced selectivity, could be the most efficient and environmentally friendly technique for converting complex lignocellulose polymers to fermentable monosaccharides, and it is expected to make cellulases and xylanases the most demanded industrial enzymes. The widespread nature of thermophilic microorganisms allows them to proliferate on a variety of substrates and release substantial quantities of cellulases and xylanases, which makes them a great source of thermostable enzymes. The most significant breakthrough of lignocellulolytic enzymes lies in lignocellulose-deconstruction by enzymatic depolymerization of holocellulose into simple monosaccharides. However, commercially valuable thermostable cellulases and xylanases are challenging to produce in high enough quantities. Thus, the present review aims at giving an overview of the most recent thermostable cellulases and xylanases isolated from thermophilic and hyperthermophilic microbes. The emphasis is on recent advancements in manufacturing these enzymes in other mesophilic host and enhancement of catalytic activity as well as thermostability of thermophilic cellulases and xylanases, using genetic engineering as a promising and efficient technology for its economic production. Additionally, the biotechnological applications of thermostable cellulases and xylanases of thermophiles were also discussed.

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“…β-glucosidases (BGLs) are a class of enzymes that hydrolyze the β-1,4-glycosidic bond of the non-reducing terminal residue of β- d -glucoside while releasing glucose. BGLs have been applied in many biotechnological processes, such as the saccharification of lignocellulosic biomass for the production of bioethanol [ 1 ], wine and juice production to enhance flavor [ 2 ], and addition to feed to improve the digestion of cellulose in farm animals [ 3 ]. Additionally, BGLs play a role in cellulose digestion and phytohormone activation and participate in the hydrolysis of mammalian glucosyl ceramides [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in β-Glucosidase Sequence and Structure Engineering: A Brief Review

et al. 2023

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β-glucosidases (BGLs) play a crucial role in the degradation of lignocellulosic biomass as well as in industrial applications such as pharmaceuticals, foods, and flavors. However, the application of BGLs has been largely hindered by issues such as low enzyme activity, product inhibition, low stability, etc. Many approaches have been developed to engineer BGLs to improve these enzymatic characteristics to facilitate industrial production. In this article, we review the recent advances in BGL engineering in the field, including the efforts from our laboratory. We summarize and discuss the BGL engineering studies according to the targeted functions as well as the specific strategies used for BGL engineering.

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Rational molecular design for improving digestive enzyme resistance of beta-glucosidase from Trichoderma viride based on inhibition of bound state formation

Cited by 10 publications

References 48 publications

Development of a New High-Cell Density Fermentation Strategy for Enhanced Production of a Fungus β-Glucosidase in Pichia pastoris

Development of a New High-Cell Density Fermentation Strategy for Enhanced Production of a Fungus β-Glucosidase in Pichia pastoris

Thermostable Cellulases / Xylanases From Thermophilic and Hyperthermophilic Microorganisms: Current Perspective

Recent Advances in β-Glucosidase Sequence and Structure Engineering: A Brief Review

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