RBCs labeled at two biotin densities permit simultaneous and repeated measurements of circulating RBC volume

Mock, Donald M.; Lankford, Gary L.; Widness, John A.; Burmeister, Leon F.; Kahn, Daniel; Strauss, Ronald G.

doi:10.1111/j.1537-2995.2004.00654.x

Cited by 23 publications

(52 citation statements)

References 28 publications

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“…Advantages include: 1) the biotinylating reagents required are inexpensive and commercially available; 2) the required measurement and synthesis equipment (a flow cytometer and sterile hood) are readily available; and 3) technician time required for analysis is modest (18, 19). Labeling RBCs with biotin, an essential B vitamin, has been shown to be nontoxic (11), and there have been no adverse clinical consequences other than asymptomatic, transient antibody formation in 15 to 20% of adults (20, 21).…”

Section: Discussionmentioning

confidence: 99%

Tracking donor RBC survival in premature infants: agreement of multiple populations of biotin-labeled RBCs with Kidd antigen–mismatched RBCs

et al. 2013

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BackgroundAnemia, a common condition among critically ill premature infants, is affected by red blood cell (RBC) survival (RCS). We hypothesized that transfused allogeneic Kidd antigen mismatched RBCs would demonstrate the same concurrent RCS tracking as RBCs multi-labeled at separate, discrete low densities with biotin (BioRBCs).MethodsAllogeneic RBCs from adult donors were labeled at four biotin densities, mixed, and transfused into 17 anemic premature infants. Nine of the donors and neonates were Kidd antigen mismatched. Serial post-transfusion blood samples were assayed for up to eight weeks by flow cytometry to track the survival of the proportions of Kidd antigen mismatched and biotinylated RBCs.ResultsUsing linear mixed modeling to compare results, RCS of the three lowest BioRBC densities was similar to RCS by Kidd antigen mismatch and to one another. RCS of RBCs labeled at the highest BioRBC density was shortened.ConclusionsRCS of different populations of RBCs can be tracked concurrently and reliably using the three lowest BioRBC densities. Although comparable RCS results can be achieved using Kidd antigen mismatches, BioRBCs are preferred for investigating neonatal anemias because biotin labeling of both allogeneic and autologous RBCs is possible.

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Section: Discussionmentioning

confidence: 99%

Tracking donor RBC survival in premature infants: agreement of multiple populations of biotin-labeled RBCs with Kidd antigen–mismatched RBCs

et al. 2013

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“…Thus, the distinct RBC subpopulations labeled with different densities of biotin can potentially be used to measure RCV simultaneously or repetitively in the same subject. Because the densities are independently enumerated, no base line correction8 is needed. Such serial measurements would assist in quantitative estimates of erythropoiesis despite the intervening events such as hemmorhage, transfusion, and somatic growth that typically confounds attempts to quantify erythropoiesis in clinical situations.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the biotin method is practical because 1) the required biotinylation reagents are inexpensive and commercially available, 2) the required instruments (a flow cytometer and sterile hood) are available in most medical center clinical laboratories, and 3) technician time required for analysis is modest 8,9…”

Section: Introductionmentioning

confidence: 99%

Red blood cell (RBC) volume can be independently determined in vivo in the sheep using ovine RBCs labeled at different densities of biotin

Mock

Matthews²,

Zhu³

et al. 2010

Transfusion

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BACKGROUND-To investigate the pathophysiology of anemia and responses to RBC transfusions and erythropoietin, repeated measurement of the circulating red cell volume (RCV) would be useful. Ovine erythropoiesis is similar to human erythropoiesis. Accordingly, a method for measuring RCV using either human or sheep RBCs labeled at different biotin densities has been previously validated in vitro. Here preclinical studies validating this method for in vivo measurement of circulating RCV in sheep are reported.

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“…As long as the peaks representing the fluorescent-labeled BioRBC population(s) remains separate from the unlabeled RBC and from each other, enumeration of the proportion of BioRBC can be accurately determined. Consequently, loss of label does not limit accuracy in determining RCV [5, 6, 55] or RCS under conditions of either normal survival [53] or accelerated removal [50]. …”

Section: Methods For Biotin Rbc Labelingmentioning

confidence: 99%

Measurement of Posttransfusion Red Cell Survival With the Biotin Label

Mock

Widness

Veng‐Pedersen

et al. 2014

Transfusion Medicine Reviews

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The goal of this review is to summarize and critically assess information concerning the biotin method to label red blood cells (RBC) for use in studies of RBC and transfusion biology — information that will prove useful to a broad audience of clinicians and scientists. A review of RBC biology, with emphasis on RBC senescence and in vivo survival is included, followed by an analysis of the advantages and disadvantages of biotin labeled RBC (BioRBC) for measuring circulating RBC volume, post-transfusion RBC recovery, RBC lifespan, and RBC age-dependent properties. The advantages of BioRBC over 51Cr RBC labeling, the current reference method, are discussed. Because the biotin method is straightforward and robust, including the ability to follow the entire lifespans of multiple RBC populations concurrently in the same subject, BioRBC offers distinct advantages for studying RBC biology and physiology, particularly RBC survival. The method for biotin labeling, validation of the method, and application of BioRBCs to studies of sickle cell disease, diabetes, and anemia of prematurity are reviewed. Studies documenting the safe use of BioRBC are reviewed; unanswered questions requiring future studies, remaining concerns, and regulatory barriers to broader application of BioRBC including adoption as a new reference method are also presented.

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RBCs labeled at two biotin densities permit simultaneous and repeated measurements of circulating RBC volume

Abstract: Using RBCs labeled with two different densities of biotin, RBC volume can be accurately measured simultaneously and repeatedly in the same subject without radiation exposure.

Cited by 23 publications

References 28 publications

Tracking donor RBC survival in premature infants: agreement of multiple populations of biotin-labeled RBCs with Kidd antigen–mismatched RBCs

Tracking donor RBC survival in premature infants: agreement of multiple populations of biotin-labeled RBCs with Kidd antigen–mismatched RBCs

Red blood cell (RBC) volume can be independently determined in vivo in the sheep using ovine RBCs labeled at different densities of biotin

Measurement of Posttransfusion Red Cell Survival With the Biotin Label

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