RDM1 plays an oncogenic role in human ovarian carcinoma cells

Abstract

Ovarian cancer is one of the deadliest gynecological cancer, with a low overall 5-year survival rate. RDM1, RAD52 motif-containing protein 1, is sensitive to cisplatin, a common chemotherapy drug and it has an important role inDNA damage repair pathway. Until now, the effect of RDM1 in ovarian cancer is undiscovered. Here, clinical data shows that the tumour tissues of ovarian carcinoma patients with higher mRNA and protein expression of RDM1. Knockdown of RDM1 in ovarian carcinoma cells reduces cell proliferation and promotes apoptosis, consistent with the role RDM1 in the overexpression experiments. The research of xenograft mouse model shows stable knockdown of RDM1 significantly inhibits ovarian cancer tumour growth. These in vitro and in vivo results conclude that RDM1 plays an oncogenic role in human ovarian carcinoma. Interestingly, p53/RAD51/RAD52 signalling pathway can be regulated by RDM1, and the negative regulation of p53 by RDM1 may be one of major mechanisms for RDM1 to accomplish its oncogenic functions in ovarian carcinoma. Therefore, RDM1 may be a new target for the treatment of ovarian carcinoma.

Multi-Omics Approaches for Revealing the Epigenetic Regulation of Histone H3.1 during Spermatogonial Stem Cell Differentiation In Vitro

“…Red and blue dots represented highly- and scarcely-expressed genes at 48 h, respectively ( Figure 4 E–H). We also revealed that the genes with relatively high expression at 0 h were primarily related to cell growth and proliferation, particularly cancer cell proliferation, such as Tfap4 [ 17 ], Rdm1 [ 18 ], Ddx39b [ 19 ], Pgam1 [ 20 ], and so on. Some of the highly expressed genes at 48 h were related to growth inhibition and differentiation, such as Maf1 [ 21 ], Zfp771 [ 22 ], Smarcd1 [ 23 ], Hoxb4 [ 24 ], and so on ( Figure 4 E).…”

The genetic architecture of complete blood counts in lactating Holstein dairy cows

“…Previous research on humans has shown that the RDM1 gene is involved in mediating DNA damage repair through homologous recombination and the cellular response to a chemotherapy drug ( Hamimes et al, 2005 ; Hamimes et al, 2006 ; Messaoudi et al, 2007 ). Moreover, knockdown of this gene reduced the proliferation of tumor cells, increased cell apoptosis, and induced cell cycle arrest ( Li et al, 2017 ; Xu et al, 2018 ; Chen et al, 2019 ; Tong et al, 2020 ). The expression of RDM1 is correlated with the degree of immune infiltration of immune cells, including macrophages and neutrophils, in a variety of cancer types ( Qui et al, 2021 ).…”

Correlation analysis of RDM1 gene with immune infiltration and clinical prognosis of hepatocellular carcinoma

“…As cancer progresses, more mutations accumulate in the DNA repair proteins of the tumor (41). Previous studies have shown that RDM1 potentiates RAD52-RAD51 signaling via p53-mediated transcriptional suppression (20,21). P53 plays an important role in regulating cell growth and apoptosis by triggering cell growth arrest or apoptosis through the regulation of various downstream targets (42).…”

“…Although there are few reports on the developmental role of RDM1 in tumors, studies have highlighted that RDM1 is upregulated in papillary thyroid cancer, non-small cell lung cancer, ovarian cancer and breast cancer. Knockdown of RDM1 can reduce the proliferation of tumor cells, increase cell apoptosis, and induce cell cycle arrest (18)(19)(20)(21). However, the role of RDM1 in LIHC remains largely unexplored.…”