rDNA analyses as an aid to the taxonomy of species of Ganoderma

Gottlieb, Alexandra M.; Ferrer, Esther; Wright, Jorge E.

doi:10.1017/s095375620000304x

Cited by 62 publications

(43 citation statements)

References 36 publications

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“…Structure of upper surface Aspect and structure of the abhymenial surface is a discriminating morphological feature of (Steyaert 1980;Gottlieb et al 1999;Moncalvo 2000;Welti and Courtecuisse 2010). In the Trametes group differences in pileus-structure (glabrous or tomentose) have already been described for each species studied here and are considered by Laessøe and Ryvarden (2010) as an essential feature for species recognition; they nevertheless never been used for phylogenetic interpretation.…”

Section: Morphological Characters In the Four Branches Within The Tramentioning

confidence: 99%

Molecular phylogeny of Trametes and related genera, and description of a new genus Leiotrametes

Welti

Moreau²,

Favel

et al. 2012

Fungal Diversity

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A phylogenetic analysis of Trametes and related taxa is proposed, based on a wide sampling of temperate and tropical species. Concatenate sequences of ribosomal DNA (ITS1-5.8S-ITS2) and RPB2 gene from mycelia cultures were analyzed by Maximum Likelihood and Bayesian methods, whilst morphological features were documented from the corresponding herbarium vouchers. Congruent results were obtained from analyses of ribosomal LSU sequences downloaded from Genbank. The Bayesian analysis of ITS + RPB2 sequences gave the best resolution for the phylogenetic reconstructions and revealed the existence of three main lineages in the Trametes-

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Section: Morphological Characters In the Four Branches Within The Tramentioning

confidence: 99%

Molecular phylogeny of Trametes and related genera, and description of a new genus Leiotrametes

Welti

Moreau²,

Favel

et al. 2012

Fungal Diversity

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“…This could be attributed to the difficulties in visualizing fragments smaller than 50 bp or comigrating of same-sized fragments on the gel [3] . Another possible reason could be small fragments may be unresolved or lost during gel electrophoresis [8] . The total sizes of the restriction fragments were larger than the undigested fragment when digestions were conducted using Bsu 15I, Hin 6I, Eco RI and Eco 88I.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Fusarium Oxysporum F. Sp. Cubense of Banana

Leong¹,

Zakaria²,

Baharuddin³

2009

American J. of Applied Sciences

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Problem statement: Morphological characterization of Fusarium species which emphasize on microscopic and cultural characteristics are not sufficient to characterize Fusarium Oxysporum F. sp. Cubense (FOC) from banana as these characteristics could easily influence by environmental factors. As an alternative molecular methods were used to characterize and to assess genetic variation of FOC from different banana cultivars. Knowledge on the genetic variation is important to determine the genetic relationship between FOC isolates from different banana cultivars. Approach: Two PCRbased methods, Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) and restriction analysis of the Internal Transcribed Spacer and 5.8S regions (ITS+5.8S regions) were used to characterize Fusarium Oxysporum F. sp. Cubense (FOC) isolates from different banana cultivars. The genetic relationship of the FOC isolates were analyzed using Un-weighted Pair-Group Method with Arithmetic Averages (UPGMA) cluster analysis based on Jaccard Similarity Coefficient. Results: Restriction patterns of the ITS+5.8S regions using nine restriction enzymes namely, Alu I, Eco RI, Eco 88I, Bsu RI, Bsu 15I, Hin fI, Hin 6I, Msp I and Taq I and ERIC-PCR showed low variation among the FOC isolates studied, indicating close relationship among the isolates. Unweighted Pair-group Method with Arithmetic Averages (UPGMA) cluster analysis based on Jaccard Similarity Coefficient showed that the FOC isolates were grouped into two main clusters with similarity value of 41.4-100% in PCR-RFLP of ITS + 5.8S and 45-100% similarity based on ERIC-PCR analysis, respectively. Cluster analysis of the combined data also showed that the FOC isolates were grouped into two clusters, sharing 42.9-100% similarity. Conclusion/Recommendations: The results of the present study indicate that the FOC isolates were closely related regardless of banana cultivars and location.

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“…However, the change of fruiting body color from the same genus can be greatly affected by growth conditions. As a result, the concept of species in this genus has not been well established nor universally accepted (Gottlieb et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…These methods include hybridization-based methods (Luo et al, 2005), polymerase chain reaction (PCR)-based methods (Hseu et al, 1996;Luo et al, 2005;Zheng et al, 2007;Wu et al, 2009), and nuclear sequencing-based methods. These sequences contain the nucleotide sequences of a 25S-28S nuclear large subunit ribosomal DNA (rDNA) (Moncalvo et al, 1995a;Lee et al, 2006), 5.8S nuclear small subunit (SSU) rDNA (Latiffah et al, 2002), nuclear internal transcribed spacer (ITS) region rDNA (Moncalvo et al, 1995b;Gottlieb et al, 2000;Smith and Sivasithamparam, 2000;Latiffah et al, 2002), and a specific gene (Zhou et al, 2008) using nuclear sequencing-based methods. Although phylogenetic studies of nuclear rDNA sequences have provided insights into the relationships among Ganoderma species, the relationships between this genus and other genera of the Polyporaceae remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of widely cultivated Ganoderma in China based on the mitochondrial V4-V6 region of SSU rDNA

Zhou¹,

Su²,

Zhang³

2015

Genet. Mol. Res.

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ABSTRACT. Ganoderma mushroom is one of the most prescribed traditional medicines and has been used for centuries, particularly in China, Japan, Korea, and other Asian countries. In this study, different strains of Ganoderma spp and the genetic relationships of the closely related strains were identified and investigated based on the V4-V6 region of mitochondrial small subunit ribosomal DNA of the Ganoderma species. The sizes of the mitochondrial ribosomal DNA regions from different Ganoderma species showed 2 types of sequences, 2.0 or 0.5 kb. A phylogenetic tree was constructed, which revealed a high level of genetic diversity in Ganoderma species. Ganoderma lucidum G05 and G. eupense G09 strains were clustered into a G. resinaceum group. Ganoderma spp G29 and G22 strains were clustered into a G. lucidum group. However, Ganoderma spp G19, G20, and G21 strains were

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rDNA analyses as an aid to the taxonomy of species of Ganoderma

Cited by 62 publications

References 36 publications

Molecular phylogeny of Trametes and related genera, and description of a new genus Leiotrametes

Molecular phylogeny of Trametes and related genera, and description of a new genus Leiotrametes

Molecular Characterization of Fusarium Oxysporum F. Sp. Cubense of Banana

Phylogenetic analysis of widely cultivated Ganoderma in China based on the mitochondrial V4-V6 region of SSU rDNA

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