rDNA-based characterization of a new binucleate Rhizoctonia spp. causing root rot on kale in Brazil

Kuramae, Eiko E.; Buzeto, Alexandre Luis; Nakatani, Andréia Kazumi; Souza, Nilton Luiz de

doi:10.1007/s10658-007-9175-z

Cited by 14 publications

(8 citation statements)

References 29 publications

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“…AG determinations confirmed that the BNR isolates from this study that grouped within the same clade belonged to the same AG, while isolates in other clades belonged to different AGs. It has been demonstrated previously that grouping of Rhizoctonia isolates by molecular analysis based on the ITS sequences supports the AG grouping of Rhizoctonia isolates based on classical hyphal anastomosis reactions [12], [15], [16], [30]. This study confirms that molecular analysis based on the ITS sequences is appropriate for evaluating genetic diversity and characterizing potential AG groups of Rhizoctonia isolates.…”

Section: Discussionsupporting

confidence: 82%

“…are soil-borne fungal pathogens with a worldwide distribution [14], [15]. They can cause severe damage to a wide range of economically important agricultural and horticultural crops [16]–[18]. Disease caused by Rhizoctonia spp.…”

Section: Introductionmentioning

confidence: 99%

“…[12], [13], [15], [16], [29], [31], [32]. The ITS sequences lie between the 18S and 5.8S rRNA genes (ITS1) and between the 5.8S and 28S rRNA genes (ITS2).…”

Section: Introductionmentioning

confidence: 99%

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Wide Variation in Virulence and Genetic Diversity of Binucleate Rhizoctonia Isolates Associated with Root Rot of Strawberry in Western Australia

2013

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Strawberry (Fragaria×ananassa) is one of the most important berry crops in the world. Root rot of strawberry caused by Rhizoctonia spp. is a serious threat to commercial strawberry production worldwide. However, there is no information on the genetic diversity and phylogenetic status of Rhizoctonia spp. associated with root rot of strawberry in Australia. To address this, a total of 96 Rhizoctonia spp. isolates recovered from diseased strawberry plants in Western Australia were characterized for their nuclear condition, virulence, genetic diversity and phylogenetic status. All the isolates were found to be binucleate Rhizoctonia (BNR). Sixty-five of the 96 BNR isolates were pathogenic on strawberry, but with wide variation in virulence, with 25 isolates having high virulence. Sequence analysis of the internal transcribed spacers of the ribosomal DNA separated the 65 pathogenic BNR isolates into six distinct clades. The sequence analysis also separated reference BNR isolates from strawberry or other crops across the world into clades that correspond to their respective anastomosis group (AG). Some of the pathogenic BNR isolates from this study were embedded in the clades for AG-A, AG-K and AG-I, while other isolates formed clades that were sister to the clades specific for AG-G, AG-B, AG-I and AG-C. There was no significant association between genetic diversity and virulence of these BNR isolates. This study demonstrates that pathogenic BNR isolates associated with root rot of strawberry in Western Australia have wide genetic diversity, and highlights new genetic groups not previously found to be associated with root rot of strawberry in the world (e.g., AG-B) or in Australia (e.g., AG-G). The wide variation in virulence and genetic diversity identified in this study will be of high value for strawberry breeding programs in selecting, developing and deploying new cultivars with resistance to these multi-genetic groups of BNR.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

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Wide Variation in Virulence and Genetic Diversity of Binucleate Rhizoctonia Isolates Associated with Root Rot of Strawberry in Western Australia

2013

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“…R . solani AG 4 has been classified into subgroups HG‐I, HG‐II and HG‐III based on sclerotial form and rDNA‐ITS nucleotide sequences (Kuninaga & Yokosawa, ; Kuramae, Buzeto, Nakatani, & Souza, ).…”

Section: Introductionmentioning

confidence: 99%

Characterization and pathogenicity ofRhizoctoniaspp. isolated from kiwifruit in the Middle and Eastern Black Sea region of Turkey

Türkkan

Erper

Kılıçoğlu

et al. 2018

Journal of Phytopathology

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In this study, 58 Rhizoctonia isolates were obtained from plants exhibiting symptoms of root and stem rot in kiwifruit orchards in six different provinces in the Black Sea region of Turkey. The isolates were analysed for their cultural characteristics, anastomosis groups (AGs) and subgroups according to nucleotide sequence variations in the internal transcribed spacer (ITS) regions including the 5.8S gene of nuclear ribosomal DNA (rDNA) and pathogenicity. Based on ITS1‐5.8S‐ITS2 rDNA sequencing, 52 binucleate (BN) Rhizoctonia isolates were identified as belonging to AG‐O (17), AG‐L (1), AG‐G (7), AG‐I (1), AG‐A (9), AG‐Fa (9), AG‐Fb (1), AG‐R (3) and AG‐P (4). Of the remaining 6 isolates, three belonged to multinucleate (MN) R. solani AG 4 HG‐I, one belonged to AG 4 HG‐II, one belonged to AG 5 and one belonged to AG 1‐IB. The sequences of these isolates were aligned and compared with other Rhizoctonia sequences retrieved from GenBank (NCBI), and phylogenetic analysis was used to determine evolutionary relationships among them. All isolates used in the temperature experiments grew between 15–35°C, and their growth was completely inhibited at 5 and 40°C, with a few exceptions. In the pathogenicity tests carried out using 58.6% of the isolates on kiwifruit seedlings, it was determined that there were statistically significant virulence differences among BN and MN Rhizoctonia isolates (p < 0.05). The virulence of BN Rhizoctonia ranged from 0.2 to 4.0, whereas that of MN varied from 2.4 to 4.0. Unlike some isolates of AG‐R, AG 4 HG‐II and AG 1‐IB, which had no significant difference between them in virulence, AG‐P and AG 4 HG‐I isolates significantly reduced plant height, shoot and root dry weights and root length when compared to control plants. To our knowledge, it is the first detailed report of AGs of BN and MN Rhizoctonia, the causal agent of root and stem rot on kiwifruit in Turkey and in the world.

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“…AG2-2 has also been associated with leaf blight of endive in California, USA (Koike & Subbarao, 1999), AG4 has been recorded on lamb's lettuce in Italy (Gullino et al, 2019), isolates from spinach in Brazil belonged to AG4-HGIII (Kuramae et al, 2003), and a binucleate Rhizoctonia sp. causing hypocotyl and root rot in kale has also been reported in Brazil (Kuramae et al, 2007). In Greece, although the pathogen has been isolated from lettuce and spinach (Farr & Rossman, 2021;Holevas et al, 2000), there are no reports describing the AGs of R. solani in these crops.…”

mentioning

confidence: 98%

Molecular identification and pathogenicity of Rhizoctonia solani and Pythium spp. associated with damping‐off disease on baby leafy vegetables in Greece

Tziros

Karaoglanidis

2022

Plant Pathology

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Leafy vegetables have gained in popularity as they provide consumers with a variety of products of high nutritional value and different organoleptic characteristics such as colour, taste and texture (Koukounaras et al., 2020). These vegetables form an important part of the "ready to eat" salad sector also known as fresh-cut or bagged salads (Gullino et al., 2019). In the last few years, mainly because of their appealing presentation, most leafy vegetables are offered in the market as fresh-cut or baby leaves rather than as intact products (Martínez-Sánchez et al., 2012). The most widely cultivated leafy vegetables in Greece are lettuce, wild and cultivated rocket, endive, lamb's lettuce, spinach and table beet. Recently, new crop species such as kale, mizuna, mustard plant, tatsoi and cardamom plant have been introduced. In Greece, leafy vegetables are cultivated in the open field or, more often, under protection in multitunnels or in greenhouses.

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rDNA-based characterization of a new binucleate Rhizoctonia spp. causing root rot on kale in Brazil

Cited by 14 publications

References 29 publications

Wide Variation in Virulence and Genetic Diversity of Binucleate Rhizoctonia Isolates Associated with Root Rot of Strawberry in Western Australia

Wide Variation in Virulence and Genetic Diversity of Binucleate Rhizoctonia Isolates Associated with Root Rot of Strawberry in Western Australia

Characterization and pathogenicity ofRhizoctoniaspp. isolated from kiwifruit in the Middle and Eastern Black Sea region of Turkey

Molecular identification and pathogenicity of Rhizoctonia solani and Pythium spp. associated with damping‐off disease on baby leafy vegetables in Greece

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