ABSTRACT-Online Bioinformatics tool needs access through the World Wide Web, which requires Internet service. To overcome this we programmed a new offline tool, ProGene1.0 for advanced protein and nucleotide analysis. ProGene 1.0 developed by programming language Microsoft Visual Basic 6.0 designed to predict chemical properties, Molecular mass, Isoelectric point, Functional group variation, Molecular composition, Nucleotide composition and Nucleotide to protein conversion. ProGene 1.0 is a desktop based tool that allows researchers to get the sequence level information on a novel and unknown or hypothetical protein or nucleotide.