Reach out and touch someone: potential impact of DIET (direct interspecies energy transfer) on anaerobic biogeochemistry, bioremediation, and bioenergy

Lovley, Derek R.

doi:10.1007/s11157-011-9236-9

Cited by 158 publications

(106 citation statements)

References 26 publications

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“…This was similar to the previous studies that have reported the enrichment of Geobacter species in aquifer sediments by the addition of humics and other extracellular quinones [43]. The genus Geobacter has been surprisingly discovered to be capable of forming highly conductive network of pilis that facilitate long-range electron transfer [34,38,39], which is becoming an important feature of Geobacter species in anaerobic environments [17,26,27]. There is increasing evidence that Geobacter species are important syntrophic microorganisms capable of establishing syntrophic association with methanogens [19,29,45].…”

Section: Discussionsupporting

confidence: 89%

Extracellular Quinones Affecting Methane Production and Methanogenic Community in Paddy Soil

Yang

et al. 2013

Microb Ecol

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This study investigated the change of CH 4 production and methanogenic community in response to the presence of humic substances (humics) analogue, anthraquinone-2,6-disulfonate (AQDS). Anaerobic experiments used a Chinese paddy soil, and three concentration levels of 0.5, 5, and 20 mM AQDS were conducted. Results suggested that the effect of AQDS on methanogenesis was time-dependent and concentration-dependent. Twenty millimolars of AQDS was toxic for methanogenic activity almost for the entire experimental period. Slight inhibition of methanogenesis by AQDS respiration in the 0.5-and 5-mM AQDS-supplemented treatments occurred within the early period, while CH 4 accumulated throughout the later period was approximately five and ten times greater than that of the controls without AQDS, respectively. AQDS reduction coupling to acetate oxidization enriched Geobacter species, and the mcrA-targeted T-RFLP profiles revealed significant increase of Methanosarcina at the expense of Methanobacterium in the 0.5-and 5-mM AQDS treatments. The enriched syntrophic association between Geobacter and Methanosarcina was deduced to be an effective methanogenic pathway for converting acetate to CH 4 via direct interspecies electron transfer. This study implied the ecological importance of syntrophic interaction between methanogens and microorganisms enriched by anaerobic respiration of non-methanogenic terminal electron acceptors in paddy soils.

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Section: Discussionsupporting

confidence: 89%

Extracellular Quinones Affecting Methane Production and Methanogenic Community in Paddy Soil

Yang

et al. 2013

Microb Ecol

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“…Direct interspecies electron transfer (DIET) is a newly described alternative in which electrons are transferred from the electron-donating partner to the electron-accepting partner via biological electrical connections (32)(33)(34)(35)(36)(37) or conductive materials (16-19, 22, 38-40). DIET has been considered more effective than hydrogen/formate interspecies electron transfer due to the elimination of multiple enzymatic steps involved in hydrogen production and consumption (41)(42)(43). Methanosaeta harundinacea (36) and M. barkeri (37) are the only methanogens known to be capable of DIET.…”

Section: Fig 2 Xrd Spectra Of Ferrihydrite Incubated With Geobacter Mmentioning

confidence: 99%

Secondary Mineralization of Ferrihydrite Affects Microbial Methanogenesis in Geobacter-Methanosarcina Cocultures

Tang

et al. 2016

Appl Environ Microbiol

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The transformation of ferrihydrite to stable iron oxides over time has important consequences for biogeochemical cycling of many metals and nutrients. The response of methanogenic activity to the presence of iron oxides depends on the type of iron mineral, but the effects of changes in iron mineralogy on methanogenesis have not been characterized. To address these issues, we constructed methanogenic cocultures of Geobacter and Methanosarcina strains with different ferrihydrite mineralization pathways. In this system, secondary mineralization products from ferrihydrite are regulated by the presence or absence of phosphate. In cultures producing magnetite as the secondary mineralization product, the rates of methanogenesis from acetate and ethanol increased by 30.2% and 135.3%, respectively, compared with a control lacking ferrihydrite. Biogenic magnetite was proposed to promote direct interspecies electron transfer between Geobacter and Methanosarcina in a manner similar to that of ctype cytochrome and thus facilitate methanogenesis. Vivianite biomineralization from ferrihydrite in the presence of phosphate did not significantly influence the methanogenesis processes. The correlation between magnetite occurrence and facilitated methanogenesis was supported by increased rates of methane production from acetate and ethanol with magnetite supplementation in the defined cocultures. Our data provide a new perspective on the important role of iron biomineralization in biogeochemical cycling of carbon in diverse anaerobic environments. IMPORTANCE It has been found that microbial methanogenesis is affected by the presence of iron minerals, and their influences on methano-

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“…Figure 1 depicts the current state of knowledge on the microbial EET at anodes-see for details e.g. 2,4,6,8,9 The EET at the cathode is still mainly untapped 10 .…”

Section: Introductionmentioning

confidence: 99%

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization

Gimkiewicz

2013

JoVE

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The growth of anodic electroactive microbial biofilms from waste water inocula in a fed-batch reactor is demonstrated using a three-electrode setup controlled by a potentiostat. Thereby the use of potentiostats allows an exact adjustment of the electrode potential and ensures reproducible microbial culturing conditions. During growth the current production is monitored using chronoamperometry (CA). Based on these data the maximum current density (j max ) and the coulombic efficiency (CE) are discussed as measures for characterization of the bioelectrocatalytic performance. Cyclic voltammetry (CV), a nondestructive, i.e. noninvasive, method, is used to study the extracellular electron transfer (EET) of electroactive bacteria. CV measurements are performed on anodic biofilm electrodes in the presence of the microbial substrate, i.e. turnover conditions, and in the absence of the substrate, i.e. nonturnover conditions, using different scan rates. Subsequently, data analysis is exemplified and fundamental thermodynamic parameters of the microbial EET are derived and explained: peak potential (E p ), peak current density (j p ), formal potential (E f ) and peak separation (ΔE p ). Additionally the limits of the method and the state-of the art data analysis are addressed. Thereby this video-article shall provide a guide for the basic experimental steps and the fundamental data analysis.

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Reach out and touch someone: potential impact of DIET (direct interspecies energy transfer) on anaerobic biogeochemistry, bioremediation, and bioenergy

Cited by 158 publications

References 26 publications

Extracellular Quinones Affecting Methane Production and Methanogenic Community in Paddy Soil

Extracellular Quinones Affecting Methane Production and Methanogenic Community in Paddy Soil

Secondary Mineralization of Ferrihydrite Affects Microbial Methanogenesis in Geobacter-Methanosarcina Cocultures

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization

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