Reactive Nitrogen and Oxygen Species in Hepatic Encephalopathy

Rivera-Mancía, Susana; Tristán-López, Luis; Montes, Sergio

doi:10.1016/b978-0-12-804274-8.00037-0

Cited by 2 publications

(1 citation statement)

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“…The effect of oxidized bases in mRNAs may cause ribosome stalling on the transcripts, leading to a decrease of protein expression or slowing the translation process. In a similar manner, an important consequence of RNA nitration could be an impairment of protein synthesis ( Rivera-Mancía et al, 2017 ). Briefly, targeted RNA nitration might lead to the diminished expression of specified proteins and thus constitute a mechanism of post-transcriptional gene expression regulation.…”

Section: Discussionmentioning

confidence: 99%

RNA and mRNA Nitration as a Novel Metabolic Link in Potato Immune Response to Phytophthora infestans

et al. 2018

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Peroxynitrite (ONOO-) exhibits a well-documented nitration activity in relation to proteins and lipids; however, the interaction of ONOO- with nucleic acids remains unknown in plants. The study uncovers RNA and mRNA nitration as an integral event in plant metabolism intensified during immune response. Using potato-avr/vr Phytophthora infestans systems and immunoassays we documented that potato immunity is accompanied by two waves of boosted ONOO- formation affecting guanine nucleotides embedded in RNA/mRNA and protein tyrosine residues. The early ONOO- generation was orchestrated with an elevated level of protein nitration and a huge accumulation of 8-nitroguanine (8-NO2-G) in RNA and mRNA pools confirmed as a biomarker of nucleic acid nitration. Importantly, potato cells lacking ONOO- due to scavenger treatment and attacked by the avr pathogen exhibited a low level of 8-NO2-G in the mRNA pool correlated with reduced symptoms of programmed cell death (PCD). The second burst of ONOO- coincided both with an enhanced level of tyrosine-nitrated proteins identified as subtilisine-like proteases and diminished protease activity in cells surrounding the PCD zone. Nitration of both RNA/mRNA and proteins via NO/ONOO- may constitute a new metabolic switch in redox regulation of PCD, potentially limiting its range in potato immunity to avr P. infestans.

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Section: Discussionmentioning

confidence: 99%