Read-out concepts for multiplexed bead-based fluorescence immunoassays on centrifugal microfluidic platforms

Riegger, L.; Grumann, M.; Nann, Thomas; Riegler, Jürgen; Ehlert, O.; Bessler, Wolfgang G.; Mittenbuehler, K.; Urban, G.; Pastewka, Lars; Brenner, Thilo; Zengerle, Roland; Ducrée, Jens

doi:10.1016/j.sna.2005.11.006

Cited by 71 publications

(67 citation statements)

References 18 publications

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“…3, B and C), signal amplification could potentially expand assay sensitivity toward the level of the most dilute protein biomarkers in serum. In particular, longwavelength emission quantum dots may provide for increased signal intensity and decreased autofluorescence due to a broad spectral shift (10 ). These and other improvements are well established in previous lab on a disk platforms, and may be incorporated into a POC incarnation with total sample-to-answer time approaching 10 min or less.…”

Section: Discussionmentioning

confidence: 93%

Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation

Schaff

Sommer

2011

Clinical Chemistry

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BACKGROUND:Centrifugal "lab on a disk" microfluidics is a promising avenue for developing portable, lowcost, automated immunoassays. However, the necessity of incorporating multiple wash steps results in complicated designs that increase the time and sample/reagent volumes needed to run assays and raises the probability of errors. We present proof of principle for a disk-based microfluidic immunoassay technique that processes blood samples without conventional wash steps.

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Section: Discussionmentioning

confidence: 93%

Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation

Schaff

Sommer

2011

Clinical Chemistry

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“…The broad generality of the centrifugal disks makes them applicable to many conventional detection modes, including matrixassisted laser desorption/ionization, 60,61 fluorescent assays using green fluorescent protein, 62 enzyme-linked immunosorbent assays ͑ELISA͒, 63 whole-cell reporter gene assays, 64 fluorescent antibody assays, 65 fluorescent detection of beads and quantum dots, 66 and blood absorbance measurements using in-disk optical paths. 67 The biodisks can be used as ionic biosensors.…”

Section: Detection Modes and Applications Of Biodisksmentioning

confidence: 99%

Invited Review Article: Review of centrifugal microfluidic and bio-optical disks

Nolte

2009

Review of Scientific Instruments

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Spinning biodisks have advantages that make them attractive for specialized biochip applications. The two main classes of spinning biodisks are microfluidic disks and bio-optical compact disks ͑BioCD͒. Microfluidic biodisks take advantage of noninertial pumping for lab-on-a-chip devices using noninertial valves and switches under centrifugal and Coriolis forces to distribute fluids about the disks. BioCDs use spinning-disk interferometry, under the condition of common-path phase quadrature, to perform interferometric label-free detection of molecular recognition and binding. The optical detection of bound molecules on a disk is facilitated by rapid spinning that enables high-speed repetitive sampling to eliminate 1 / f noise through common-mode rejection of intensity fluctuations and extensive signal averaging. Multiple quadrature classes have been developed, such as microdiffraction, in-line, phase contrast, and holographic adaptive optics. Thin molecular films are detected through the surface dipole density with a surface height sensitivity for the detection of protein spots that is approximately 1 pm. This sensitivity easily resolves a submonolayer of solid-support immobilized antibodies and their antigen targets. Fluorescence and light scattering provide additional optical detection techniques on spinning disks. Immunoassays have been applied to haptoglobin using protein A/G immobilization of antibodies and to prostate specific antigen. Small protein spots enable scalability to many spots per disk for high-throughput and highly multiplexed immonoassays.

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“…Of special recent note is an example whereby encoding has been coupled with flow cytometry [72]; two examples where QD-encoded beads have been used in lab-on-a-chip-type applications [73,74], one of which involves the use of a spinning disk format [73] and the other a microfluidic chip format [74]; and a fourth example where magnetic beads have been encoded with QDs and used to analyze gene expression [75]. In the first of these four examples, carboxylic acid-functionalized polystyrene beads were encoded by doping with controlled amounts of QDs (see Section 10.2.3) [72].…”

Section: Optical Encoding Applicationsmentioning

confidence: 99%