Real Time and Conventional PCR for Characterization of Salmonella sp. from Imported Meat to Egypt

El-Sayed, M.; Abdeeen, E.; Akiela, M. A.; Farouk, T.; Zahran, Rasha

doi:10.14737/journal.aavs/2014/2.4.199.203

Cited by 6 publications

(4 citation statements)

References 27 publications

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“…The colony characteristics of Salmonella spp. found in this study was translucent, black smooth, small round colonies on SS agar, pink color colony with black centre in XLD agar, were similar to the findings of other authors (Elsayed et al,2014;Harsojo and Sari, 2015;Hamad et al 2010). In Gram's staining, the morphology of the isolated Salmonella spp.…”

Section: Discussionsupporting

confidence: 93%

Bacteriological quality assessment of buffalo meat collected from different districts of Bangladesh with particular emphasis on the molecular detection and antimicrobial resistance of the isolated Salmonella species

Hasan

Kabir

Rahman

et al. 2018

Asian Australas. J. Food. Saf. Secur.

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The research project was conducted to assess the bacteriological quality of buffalo meat samples collected from three upazilas namely Haluaghat, Sreepur and Madhupur of Bangladesh under the districts of Mymensingh, Gazipur and Tangail respectively with particular emphasis on the molecular detection and antimicrobial resistance of the isolate Salmonella species. Total viable count (TVC), total staphylococcal count (TStaC) and total salmonella count (TSC) of meat samples were determined and the mean values of TVC, TStaC and TSC for the Haluaghat, Sreepur and Madhupur were log 8.30, log 7.94, log 8.15; log6.21, log 6.40, log 5.43 and log 4.76, log 4.82, log 4.56 CFU/gm respectively which exceeded the ICMSF recommendations values. The variation of TVC and TSC in meats of different buffalo markets was significant at 5% level where the variation of TStaC was significant at 1% level. Nevertheless no significant variation was demonstrated between the interactions of the three upazilas. Among the samples, 46.67% (n=14) were found to be associated with Salmonella spp. The Salmonella spp. were identified by observing black centered colonies on XLD agar, positive to MR test and negative to VP and Indole test. All isolates of Salmonella spp. were positive to 16s rRNA gene based PCR (574bp). All isolates of Salmonella species were susceptible to ciprofloxacin, streptomycin and gentamicin. All isolates of Salmonella spp. (n=14; 100%) were resistant to amoxicillin and few isolates also resistant to erythromycin, tetracycline, azithromycin and cephradine. The findings of this study revealed the presence of multidrug resistant Salmonella spp. in buffalo meat of Mymensingh, Gazipur and Tangail districts of Bangladesh that posseses a serious threat to public health. Asian Australas. J. Food Saf. Secur. 2018, 2(1), 12-20

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Section: Discussionsupporting

confidence: 93%

Bacteriological quality assessment of buffalo meat collected from different districts of Bangladesh with particular emphasis on the molecular detection and antimicrobial resistance of the isolated Salmonella species

Hasan

Kabir

Rahman

et al. 2018

Asian Australas. J. Food. Saf. Secur.

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“…Higher incidences of Salmonella spp., of 80%, and 46.67%, however, were recorded in buffalo meat in Laos [ 26 ], and Bangladesh [ 27 ], respectively. Conversely, lower incidences of 7% [ 28 ], 7.11% [ 29 ], 18% [ 30 ], 10.66% [ 31 ], and 7.4% [ 32 ] were reported for Salmonella in buffalo meat samples from Iran, Laos, Egypt, India, and Nepal, respectively.…”

Section: Resultsmentioning

confidence: 99%

Prevalence of Multidrug-Resistant Salmonella enterica Serovars in Buffalo Meat in Egypt

Abd-Elghany

Fathy

Zakaria

et al. 2022

Foods

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The current study aimed to investigate the presence of Salmonella spp. prevalence in buffalo meat in Egypt, along with studying the antimicrobial susceptibility of the recovered isolates. Salmonella spp. was detected in 25% of tested buffalo meat. A total of 53 (100%) isolates were genetically verified by PCR as Salmonella, based on the detection of the invA gene. The stn and hilA genes were detected in 71.7% (38/53), and 83.0% (44/53) of the recovered isolates, respectively. Salmonella Enteritidis (11/53; 20.7%) was the most commonly isolated serovar, followed by S. Typhimurium (9/53; 17%), S. Montevideo (6/53; 11.3%), meanwhile, S. Chester, S. Derby, S. Papuana, and S. Saintpaul were the least commonly identified serovars (a single strain for each; 1.9%). Among the 16 antimicrobials tested, amikacin, imipenem, gentamicin, cefotaxime, meropenem, ciprofloxacin, and enrofloxacin were the most effective drugs, with bacterial susceptibility percentages of 98.1%, 94.3%, 92.5%, 86.8%, 83.0%, 73.6%, and 69.8%, respectively. Meanwhile, the least effective ones were erythromycin, streptomycin, clindamycin, cefepime, and nalidixic acid, with bacterial resistance percentages of 100%, 98.1%, 88.7%, 77.4%, and 66%, respectively. Interestingly, the high contamination level of Egyptian buffalo meat with multidrug-resistant Salmonella (79.2%; 42/53) can constitute a problem for public health. Therefore, programs to control Salmonella contamination are needed in Egypt.

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“…From the result recorded in table 5 it's clear that 3 Salmonella serovars were identified in the examined frozen meat samples and identified as 2 (6.7%) strains as S. papuana, 1 (3.3%) strain as S. paratyphi A and 1 (3.3%) strain as S. vircho. Higher results were reported by Elsayed MS, et al [46] where they stated that S. paratyphi A was detected in 8/18 (44.44%) positive samples.…”

Section: Serotyping Of the Isolated Salmonellaementioning

confidence: 59%

“…Nearly similar results were recorded by El-Kader HA, et al [29] (10%), but higher results reported by AL-Jobori KM, et al [7] (40%) and Mahmood NR, et al [45] who isolated Salmonellae spp. at a rate of 24.76% and Elsayed MS, et al (18%) [46].…”

Section: Introductionmentioning

confidence: 97%

Molecular Diagnosis of Salmonellae in Frozen Meat and Some Meat Products

Shaltout¹,

El-Hai²

2019

Nutr Food Technol Open Access

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Volume 5 -Issue 1 | processing, distribution and storage as well as marketing. Such contamination may render the products of inferior quality or even unfit for human consumption and at times may constitute a public health hazard. The possibility of contamination of meat products with food poisoning bacteria especially Salmonellae organisms has been extensively reported [5].Traditional and conventional methods for identification of microbial pathogens depend on specific bacteriological and biochemical identification [6], which are time consuming, laborious and less accurate [7] and slow because Salmonellae needs 5-7 days to confirm its presence in meat products [8]. Several Polymerase Chain Reaction (PCR) assays have been developed by targeting various Salmonellae genes, such as invA [9,10]. The invA gene, is widely used as a target in PCR assays for Salmonella detection [11][12][13]. Materials and Methods Isolation and identification of SalmonellaeThe techniques adopted were carried out according to ICMSF (1978) [14]. Samples were cultivated for the isolation of Salmonellae species on MacConkey agar. After 24 h of incubation at 37°C, suspected colonies with typical characteristics of Salmonellae were sub-cultured on XLD (Xylose Lysine Deoxycholate) agar for 24 h at

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Real Time and Conventional PCR for Characterization of Salmonella sp. from Imported Meat to Egypt

Cited by 6 publications

References 27 publications

Bacteriological quality assessment of buffalo meat collected from different districts of Bangladesh with particular emphasis on the molecular detection and antimicrobial resistance of the isolated Salmonella species

Bacteriological quality assessment of buffalo meat collected from different districts of Bangladesh with particular emphasis on the molecular detection and antimicrobial resistance of the isolated Salmonella species

Prevalence of Multidrug-Resistant Salmonella enterica Serovars in Buffalo Meat in Egypt

Molecular Diagnosis of Salmonellae in Frozen Meat and Some Meat Products

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