Real-time and label-free biosensing using moiré pattern generated by bioresponsive hydrogel

Kim, Semin; Kim, Geehong; Ji, Yong Woo; Moon, Chae-Eun; Jung, Yuna; Lee, Jaejong; Koh, Won‐Gun

doi:10.1016/j.bioactmat.2022.11.010

Cited by 4 publications

(2 citation statements)

References 48 publications

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“…These changes can then be detected through microscopy. No optical quality testing was conducted [72], but this mechanism is likely not as visually intrusive as intraocular fluorescence. PCO is a common post-cataract surgery complication developing from the migration of lens epithelial cells (LECs) from the periphery to the visual axis of the lens posterior capsule [73].…”

Section: Structural and Functional Benefits Of Nanoporous Intraocular...mentioning

confidence: 99%

Exploring the Potential of Nanoporous Materials for Advancing Ophthalmic Treatments

Wu,

Brister,

Bélanger

et al. 2023

IJMS

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The landscape of ophthalmology is undergoing significant transformations, driven by technological advancements and innovations in materials science. One of the advancements in this evolution is the application of nanoporous materials, endowed with unique physicochemical properties ideal for a variety of ophthalmological applications. Characterized by their high surface area, tunable porosity, and functional versatility, these materials have the potential to improve drug delivery systems and ocular devices. This review, anchored by a comprehensive literature focusing on studies published within the last five years, examines the applications of nanoporous materials in ocular drug delivery systems (DDS), contact lenses, and intraocular lenses. By consolidating the most current research, this review aims to serve as a resource for clinicians, researchers, and material scientists engaged in the rapidly evolving field of ophthalmology.

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Section: Structural and Functional Benefits Of Nanoporous Intraocular...mentioning

confidence: 99%

Exploring the Potential of Nanoporous Materials for Advancing Ophthalmic Treatments

Wu,

Brister,

Bélanger

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…It is one of the most widely used diagnostic techniques because it offers the potential for high throughput screening of diseases 12 . In order to overcome the technical challenges of shortening analysis time, improving bioanalytical performance, and increasing reproducibility, numerous approaches have been attempted by integration with functionalized nanomaterials and nanopatterns 13 , 14 . For example, lateral flow assays 15 – 18 using nanozymes as signal catalysts have shown enhanced sensitivity and wide dynamic detection range compared to conventional gold nanoparticle-based assays.…”

Section: Introductionmentioning

confidence: 99%

Amplified fluorogenic immunoassay for early diagnosis and monitoring of Alzheimer’s disease from tear fluid

Lee,

Kim,

Moon

et al. 2023

Nat Commun

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Accurate diagnosis of Alzheimer’s disease (AD) in its earliest stage can prevent the disease and delay the symptoms. Therefore, more sensitive, non-invasive, and simple screening tools are required for the early diagnosis and monitoring of AD. Here, we design a self-assembled nanoparticle-mediated amplified fluorogenic immunoassay (SNAFIA) consisting of magnetic and fluorophore-loaded polymeric nanoparticles. Using a discovery cohort of 21 subjects, proteomic analysis identifies adenylyl cyclase-associated protein 1 (CAP1) as a potential tear biomarker. The SNAFIA demonstrates a low detection limit (236 aM), good reliability (R2 = 0.991), and a wide analytical range (0.320–1000 fM) for CAP1 in tear fluid. Crucially, in the verification phase with 39 subjects, SNAFIA discriminates AD patients from healthy controls with 90% sensitivity and 100% specificity in under an hour. Utilizing tear fluid as a liquid biopsy, SNAFIA could potentially aid in long-term care planning, improve clinical trial efficiency, and accelerate therapeutic development for AD.

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Implantable Intraocular Fluorescence Sensor for Visualized Monitoring of Alzheimer's Disease

Lim,

Lee,

Kim

et al. 2024

Adv Funct Materials

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With an increase in the older individuals, the global incidence of Alzheimer's disease (AD) is increasing. Early diagnosis of AD necessitates long‐term monitoring; however, conventional diagnostic methods such as positron emission tomography (PET) and magnetic resonance imaging (MRI) are unsuitable for frequent use because of infrastructure limitations. The eye, connected to the brain via the optic nerve, offers a potential window for monitoring neurodegenerative diseases. This study presents a novel system for continuous intraocular monitoring using a fluorescent aptamer‐conjugated intraocular lens (FAIOL). FAIOL emits fluorescent signals in the presence of beta‐secretase‐1 (BACE1), an enzyme associated with amyloid beta 1–42 production. These signals can be analyzed using mobile phone applications. In vitro, experiments demonstrates that FAIOL responded to BACE1 by detecting low concentrations of the target molecule over extended periods. Ex vivo tests using porcine eyeballs shows an increase in the fluorescence signal intensity by more than 8% within 5 h in the presence of BACE1. The integrated image analysis program reduces sensor noise and provides numerical signal values, facilitating a straightforward interpretation. FAIOL holds significant potential as an innovative platform for the early diagnosis and long‐term monitoring of AD and promises improved patient outcomes through timely intervention and ongoing surveillance.

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Real-time and label-free biosensing using moiré pattern generated by bioresponsive hydrogel

Cited by 4 publications

References 48 publications

Exploring the Potential of Nanoporous Materials for Advancing Ophthalmic Treatments

Exploring the Potential of Nanoporous Materials for Advancing Ophthalmic Treatments

Amplified fluorogenic immunoassay for early diagnosis and monitoring of Alzheimer’s disease from tear fluid

Implantable Intraocular Fluorescence Sensor for Visualized Monitoring of Alzheimer's Disease

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