Real-Time Characterization of Fibrinogen Interaction with Modified Titanium Dioxide Film by Quartz Crystal Microbalance with Dissipation

Abstract

The adsorption of fibrinogen can be used as a quick indicator of surface haemocompatibility because of its prominent role in coagulation and platelet adhesion. In this work the molecular interaction between fibrinogen and a modified titanium oxide surface/platelet has been studied by quartz crystal microbalance with dissipation (QCM-D) in situ. In order to further characterize the conformation of adsorbed fibrinogen, αC and γ-chain antibody were used to check the orientation and denaturation of fibrinogen on solid surface. QCM-D investigations revealed the fibrinogen have the trend to adsorb on hydrophilic surface in a side-on orientation by positively charged αC domains, which would reduce the exposure of platelet bonding site on γ chain and enable less platelet adhesion and be activated. These observations suggest that certain conformations of adsorbed fibrinogen are less platelet adhesive than others, which opens a possibility for creating a non-platelet adhesive substrates.

Titanium and Protein Adsorption: An Overview of Mechanisms and Effects of Surface Features

“…The strong protein–surface interactions lead to denaturation of FIB [ 66 ]. This was confirmed by Zhao et al [ 67 ]. Furthermore, even though binding via αC domains shall result in side-on orientation of the proteins on the surface, FIB was found with preferred end-on orientation [ 67 ].…”

“…This was confirmed by Zhao et al [ 67 ]. Furthermore, even though binding via αC domains shall result in side-on orientation of the proteins on the surface, FIB was found with preferred end-on orientation [ 67 ]. Bimodal adsorption isotherms of immunoglobulin (IgG) suggest that adsorbed proteins may undergo structural rearrangements and orientation modification according to saturation level of the surface [ 68 ].…”

“…With this technique, proteins adsorbed onto the surface can be weighted [ 112 ]. Different QCM set ups allow to collet further information: QCM with dissipation (QCM-D) allows to measure the dissipation of energy in the adsorbed layer, thus providing information about the flexibility and the water content of the adsorbed layer [ 67 , 126 , 227 ]; QCM can be coupled with electrochemical and impedance measurements (EQCI) [ 66 ]. Mechanisms of protein adsorption are very complex to be characterized.…”

Biomaterials for Bone Tissue Engineering

“…The strong protein-surface interactions lead to denaturation of FIB [66]. This was confirmed by Zhao et al [67]. Furthermore, even though binding via αC domains shall result in side-on orientation of the proteins on the surface, FIB was found with preferred end-on orientation [67].…”

“…Furthermore, stimulus-responsive scaffolds have attracted much interest in terms of modulating their physicochemical and mechanical properties, as well as the degradation profile in response to stimuli (including photoirradiation, mechanical, magnetic, and electrical forces, and changes in pH or enzymatic reactions) [42]. For instance, thermoresponsive biomaterials based on different polymers are tailored to have transition temperatures to gelate when the temperature is elevated to physiologic temperature [67][68][69]. Recent studies have designed piezoelectric systems that might accelerate new tissue formation in response to mechanical loading.…”