Real-time loop-mediated isothermal amplification (LAMP) assay for group specific detection of important trichothecene producing Fusarium species in wheat

Denschlag, Carla; Rieder, Johann M.; Vogel, Rudi F.; Niessen, Ludwig

doi:10.1016/j.ijfoodmicro.2014.02.010

Cited by 22 publications

(13 citation statements)

References 37 publications

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“…Trichothecene mycotoxins such as DON, nivalenol (NIV) and T‐2 toxin are produced by a variety of Fusarium spp. on cereals in the fields and may be ingested by consumption of cereal products . It was found that noninfected by F. sporotrichioides wheat grains contained from 70 µg kg −1 to 140 µg kg −1 of DON (spelt and cv.…”

Section: Resultsmentioning

confidence: 94%

Influence of microbial and chemical contaminants on the yield and quality of ethanol from wheat grains

et al. 2018

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BACKGROUND: Higher alcohols and esters have a negative impact on ethanol quality; therefore, to analyze the influence of mycotoxins and pesticides on higher alcohols and esters formation during ethanol production from contaminated cereal grains is of outstanding importance. RESULTS:In this study, the yield of ethanol and the composition of volatile by-products (acetaldehyde, methyl acetate, ethyl acetate, methanol, propanol, isobutanol, amyl and isoamyl alcohols) after fermentation of wheat grains artificially contaminated with Fusarium sporotrichioides and pesticides (triasulfuron and sulfosulfuron) were analyzed. Wheat grains contamination with triasulfuron significantly reduced ethanol yield and increased isobutanol and ethyl acetate content. Moreover, wheat grains infection with F. sporotrichioides significantly increased methyl acetate, ethyl acetate, isobutanol, and amyl and isoamyl alcohols content. Significant differences between pesticides and wheat variety on volatile compounds formation in ethanol has been estimated. CONCLUSION:The results showed that grains contaminated with F. sporotrichioides and pesticides significantly influenced the process of alcoholic fermentation that leads to variation of by-products in ethanol, including higher alcohols, esters, and aldehydes.

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Section: Resultsmentioning

confidence: 94%

Influence of microbial and chemical contaminants on the yield and quality of ethanol from wheat grains

et al. 2018

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“…As shown in Table 1, two sets of LAMP primers were designed by genes of tri6 and tri5 that are involved in the trichothecene biosynthesis. 16 It was found that each of the two above-mentioned sets could detect a limited number of the trichothecene producing Fusarium species, but a combination of the two sets in one duplex assay was able to detect all of the above-mentioned Fusarium species. Moreover, this LAMP assay revealed that all the detected samples were positive if the concentration of deoxynivaneol exceeded 1.63 × 10 −7 g, and the detection limits of genome DNA from these species varied between 0.004 ng per reaction (F. graminearum) to 15.74 ng per reaction (F. poae).…”

Section: Advanced Assays Used For the Detection Of Mycotoxin-producinmentioning

confidence: 99%

“…It has been widely used in tests of bacterial pathogens and fungal contaminants, resulting in a significant increase in the number of publications from 2000 to 2012 . Most recently, Denschlag et al developed a LAMP assay to rapidly and sensitively detect the most important trichothecene‐producing Fusarium species, including F. graminearum , F. culmorum , F. cerealis , F. sporotrichioides , F. langsethiae and F. poae . As shown in Table , two sets of LAMP primers were designed by genes of tri6 and tri5 that are involved in the trichothecene biosynthesis .…”

Section: Advanced Assays Used For the Detection Of Mycotoxin‐producinmentioning

confidence: 99%

“…Most recently, Denschlag et al developed a LAMP assay to rapidly and sensitively detect the most important trichothecene‐producing Fusarium species, including F. graminearum , F. culmorum , F. cerealis , F. sporotrichioides , F. langsethiae and F. poae . As shown in Table , two sets of LAMP primers were designed by genes of tri6 and tri5 that are involved in the trichothecene biosynthesis . It was found that each of the two above‐mentioned sets could detect a limited number of the trichothecene producing Fusarium species, but a combination of the two sets in one duplex assay was able to detect all of the above‐mentioned Fusarium species.…”

Section: Advanced Assays Used For the Detection Of Mycotoxin‐producinmentioning

confidence: 99%

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Molecular strategies for detection and quantification of mycotoxin‐producing Fusarium species: a review

2014

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Fusarium contamination is considered a major agricultural problem, which could not only significantly reduce yield and quality of agricultural products, but produce mycotoxins that are virulence factors responsible for many diseases of humans and farm animals. One strategy to identify toxigenic Fusarium species is the use of modern molecular methods, which include the analysis of DNA target regions for differentiation of the Fusarium species, particularly the mycotoxin-producing Fusarium species such as F. verticillioides and F. graminearum. Additionally, polymerase chain reaction assays are used to determine the genes involved in the biosynthesis of the toxins in order to facilitate a qualitative and quantitative detection of Fusarium-producing mycotoxins. Also, it is worth mentioning that some factors that modulate the biosynthesis of mycotoxins are not only determined by their biosynthetic gene clusters, but also by environmental conditions. Therefore, all of the aforementioned factors which may affect the molecular diagnosis of mycotoxins will be reviewed and discussed in this paper.

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“…Globally, the most dominant and aggressive pathogens are Fusarium graminearum and Fusarium culmorum, which produce the mycotoxins deoxynivalenol (DON) and nivalenol (NIV) (5); however, weaker pathogens, such as Fusarium langsethiae (6) or Fusarium poae, are able to coexist on infected hosts alongside the more aggressive species and, in doing so, contribute to the mycotoxin contamination of the grain (7). F. langsethiae is a newly identified species, first described in 2004 (8), that has been observed in Norway (9), Sweden (10), Denmark (11), the United Kingdom (12), Poland (13), Belgium (14), Italy (15), Germany (16), Russia (17), and Finland (18). There is also evidence that F. langsethiae is increasing in importance in northern Europe (19) with confirmed hosts, including oats, wheat, and barley (20).…”

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confidence: 99%

Aphid Infestation Increases Fusarium langsethiae and T-2 and HT-2 Mycotoxins in Wheat

Drakulic

Ajigboye

Swarup

et al. 2016

Appl Environ Microbiol

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Fusarium langsethiae is a fungal pathogen of cereal crops that is an increasing problem in northern Europe, but much of its epidemiology is poorly understood. The species produces the mycotoxins T-2 and HT-2, which are highly toxic. It was hypothesized that grain aphids, Sitobion avenae, may transmit F. langsethiae inoculum between wheat plants, and a series of transmission experiments and volatile chemical analyses was performed to test this. Manual translocation of aphids from inoculated to uninfected hosts resulted in pathogen DNA accumulation in hosts. However, the free movement of wingless aphids from infected to healthy plants did not. The addition of winged aphids reared on F. langsethiae-inoculated wheat seedlings to wheat plants also did not achieve successful pathogen transfer. While our data suggested that aphid transmission of the pathogen was not very efficient, we observed an increase in disease when aphids were present. After seedling inoculation, an increase in pathogen DNA accumulation in seedling leaves was observed upon treatment with aphids. Furthermore, the presence of aphids on wheat plants with F. langsethiae-inoculated ears not only led to a rise in the amount of F. langsethiae DNA in infected grain but also to an increase in the concentrations of T-2 and HT-2 toxins, with more than 3-fold higher toxin levels than diseased plants without aphids. This work highlights that aphids increase the susceptibility of wheat host plants to F. langsethiae and that aphid infestation is a risk factor for accumulating increased levels of T-2 and HT-2 in wheat products. IMPORTANCEFusarium langsethiae is shown here to cause increased contamination levels of grain with toxins produced by fungus when aphids share the host plant. This effect has also recently been demonstrated with Fusarium graminearum, yet the two fungal species show stark differences in their effect on aphid populations. In both cases, aphids improve the ability of the pathogens to cause and initiate Fusarium head blight (FHB) disease in wheat, but F. langsethiae may be able to act as a dispersal agent. F. langsethiae contributes harmful toxins to wheat grain that need to be controlled, but as yet, its epidemiology is unresolved. This work reveals insights into the role aphids play in promoting the successful colonization of this species in wheat and the benefit of controlling aphid populations on crops that are at high risk of FHB. Fusarium head blight (FHB) is a fungal disease of cereal crops that is caused by a pathogen complex comprising several species from the genus Fusarium (1) and two from the genus Microdochium (2). Fusarium species produce mycotoxins upon infecting host plants, which can be harmful if consumed by mammals, with different health impacts depending on the specific toxins produced (3). As well as causing mycotoxin contamination, infection can also lead to reduced yield and grain quality. The disease affects crops in all major cereal-growing regions worldwide, and different species prevail in different geograph...

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Real-time loop-mediated isothermal amplification (LAMP) assay for group specific detection of important trichothecene producing Fusarium species in wheat

Cited by 22 publications

References 37 publications

Influence of microbial and chemical contaminants on the yield and quality of ethanol from wheat grains

Influence of microbial and chemical contaminants on the yield and quality of ethanol from wheat grains

Molecular strategies for detection and quantification of mycotoxin‐producing Fusarium species: a review

Aphid Infestation Increases Fusarium langsethiae and T-2 and HT-2 Mycotoxins in Wheat

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