Real-time monitoring of electrochemically active biofilm developing behavior on bioanode by using EQCM and ATR/FTIR

Liu, Ying; Berná, Antonio; Climent, Vı́ctor; Feliu, Juan M.

doi:10.1016/j.snb.2014.12.047

Cited by 14 publications

(4 citation statements)

References 38 publications

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“…Moreover, atomic force microscopy (AFM) analysis of the used gold disks showed that the αAgNAs treatment removed the matrix material (Figure B), which otherwise embeds the cells on the untreated gold disk (Figure A). Taking into account the hydrolytic activity of αAgNAs (Figure ) and the reported water displacement during EPS production, the observed frequency and dissipation shifts, after the addition of αAgNAs, were attributed to an increase of the biofilm water content due to the degradation of the EPS. In fact, water exchange has been reported to compensate mass changes on the disk surface with the adherence of cells. , Furthermore, the loss of water has been associated with an increase in the strength of the cell-surface contact .…”

Section: Resultsmentioning

confidence: 94%

Metal–Enzyme Nanoaggregates Eradicate Both Gram-Positive and Gram-Negative Bacteria and Their Biofilms

Ferreres

Bassegoda

Hoyo

et al. 2018

ACS Appl. Mater. Interfaces

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To palliate the appearance of antimicrobial resistance (AMR), the use of bactericidal agents acting differently than conventional antibiotics and the elimination of bacterial biofilm, are the two most promising strategies. Here, we integrated these two complementary strategies into new antimicrobial metal–enzyme nanoaggregates (NAs) of α-amylase and silver (αAgNAs) that are able to eliminate bacteria and their biofilm. The nanoparticle (NP) synthesis approach applied protein desolvation and laccase-mediated NP stabilization to innovatively produce catalytically active α-amylase nanoparticles (αNPs) for the elimination of the bacterial biofilm. At the same time, αNPs efficiently reduced silver for the incorporation of bactericidal Ag0 and formation of the αAgNAs. The bactericidal and antibiofilm efficacies of αAgNAs were demonstrated by 5.4 and 6.1 log reduction of Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli, respectively, and more than 80% removal of their biofilms, coupled with high biocompatibility. The biofilm−αAgNA interaction was assessed by quartz crystal microbalance and atomic force microscopy revealing how the degradation of a settled biofilm by αAgNAs caused an increase of the biofilm water content, thus weakening the biofilm surface attachment and facilitating its removal. With the present work, we not only provide a new efficient antimicrobial material to face the AMR threat, but we also envisage that the newly established method for the synthesis of metal–enzyme NAs is potentially transferable to other biocatalysts to expand the enzyme NP toolbox.

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Section: Resultsmentioning

confidence: 94%

Metal–Enzyme Nanoaggregates Eradicate Both Gram-Positive and Gram-Negative Bacteria and Their Biofilms

Ferreres

Bassegoda

Hoyo

et al. 2018

ACS Appl. Mater. Interfaces

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“…These two reactions compete in the course of the electrochemical CO 2 reduction. Surface-enhanced infrared adsorption spectroscopy (SEIRAS) has been employed to investigate the mechanism of the CO 2 reduction reaction. ,− Wuttig et al revealed that bicarbonate served as a proton donor following the rate-limiting step . Dunwell et al also studied the function of bicarbonate in the electrochemical reduction of CO 2 on an Au surface using the SEIRAS technique and proposed that CO 2 reduction benefitted from the rapid equilibrium of bicarbonate species and dissolved CO 2 .…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Reduction of Carbon Dioxide on Au Nanoparticles: An in Situ FTIR Study

Chen

2019

J. Phys. Chem. C

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For the conversion and storage of renewable energy, the electrochemical reduction of CO2 to hydrocarbon fuels and chemicals comprises one of the most promising strategies. A number of studies have focused on the development of robust electrocatalysts for the reduction of CO2 with high efficiency and selectivity. Most of the proposed mechanisms of the CO2 reduction are based primarily on theoretical calculations. Although a few studies have been dedicated to the in situ direct detection of intermediates, the mechanisms are still unclear and questionable. As Au is one of the most efficient catalysts for CO2 reduction, it is selected as an electrocatalyst model for this work to examine intermediates and to explore the kinetics of the electrochemical reduction of CO2. In this study, gold nanoparticles were prepared by a facile sputtering method. In situ attenuated total reflection Fourier transform infrared spectroscopy (FTIR) was employed to investigate the reaction mechanism of the electrochemical reduction of CO2 at the Au nanoparticles. To assign the IR peaks and to identify the formed intermediates and products, H2O and D2O were used as solvents. Our experimental results have provided the direct evidence of the formation of *COO– species on the Au nanoparticles, confirming that *COO– is one of the main intermediates during the CO2 activation. Besides the main product CO, another product HCOO– was detected during the CO2 reduction on gold nanoparticles. The amount of the formed CO and formate products depends on the applied cathodic potential. The mechanism of CO and formate formation on the Au nanoparticle surface is proposed; the kinetics of the CO2 reduction has been further studied using the in situ FTIR technique. The findings from this study provide direct IR spectroscopic evidence of the CO2 reduction mechanism at the Au nanoparticles; the approaches reported in the present study would be useful to investigate various electrocatalytic reactions for energy and environmental applications.

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“…Liu's group studied the in situ interactions between the bacteria and the solid electrode during the biofilm formation at the status from early to over‐growth mature by Electrochemical Quartz Crystal Microbalance (EQCM) and EC‐SEIRAS [55]. They investigated the relationship between biomass and current according to the bio‐electrogenic process of the Geobacter sulfurreducens biofilm and found that the cellular biomass on the surface of the QCM‐crystal showed a maximum value from 6.0 to 11.5 μg/cm 2 on the biofilm from early formation status to mature status accompanied by a similar maximum current density ( ca .…”

Section: Surface‐enhanced Infrared Absorption Spectroscopy (Seiras)mentioning

confidence: 99%

Recent Advances in Plasmonic Nanostructures Applied for Label‐free Single‐cell Analysis

Liao

Tan

et al. 2021

Electroanalysis

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Cellular heterogeneity presents a major challenge in understanding the relationship between cells of particular genotype and response in disease. In order to characterize the cell‐to‐cell differences during the biochemical processes, single‐cell analysis is necessary. Profiting from the unique localized surface plasmon resonance (LSPR) and Mie scattering, plasmonic nanostructures have revealed stable and adjustable scattering signals, avoiding photobleaching, blinking and autofluorescence phenomenon. These characterizations are propitious to the dynamic trace and biological image of single living cells. In this review, we discuss the recent advances in plasmonic nanostructures applied for label‐free detection and monitoring of target cells at single‐cell level by using three different techniques, surface‐enhanced Raman scattering (SERS), surface‐enhanced Infrared absorption spectroscopy (SEIRAS), and dark‐field microscopy. Various avenues to design plasmonic probes combining spectra and imaging for single‐cell analysis are demonstrated as well. We hope this review can highlight the superiority of plasmonic nanostructures in single cellular analysis, and further motivate the development of label‐free cell analysis technique to elucidate cellular diversity and heterogeneity.

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Real-time monitoring of electrochemically active biofilm developing behavior on bioanode by using EQCM and ATR/FTIR

Cited by 14 publications

References 38 publications

Metal–Enzyme Nanoaggregates Eradicate Both Gram-Positive and Gram-Negative Bacteria and Their Biofilms

Metal–Enzyme Nanoaggregates Eradicate Both Gram-Positive and Gram-Negative Bacteria and Their Biofilms

Electrochemical Reduction of Carbon Dioxide on Au Nanoparticles: An in Situ FTIR Study

Recent Advances in Plasmonic Nanostructures Applied for Label‐free Single‐cell Analysis

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