Real-time, multiplexed holographic tomography

Kuś, Arkadiusz

doi:10.1016/j.optlaseng.2021.106783

Cited by 18 publications

(6 citation statements)

References 42 publications

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“…In the future, it is possible to take advantage of the large amount of data present in this double six-pack technique to increase the number of images acquired and thereby increase the reconstruction accuracy, at the expense of spatial bandwidth per image, by dividing each of the 12 CC terms into multiple subterms, as seen in ref ( 18 ). By utilizing our 12-fold increase in dynamic tomographic data in this manner, the double six-pack tomography technique outlined in this work is expected to lead to dynamic single-shot tomography at accuracies comparable to that of scanning tomography of static samples.…”

Section: Discussionmentioning

confidence: 99%

Dynamic Tomographic Phase Microscopy by Double Six-Pack Holography

2022

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Three-dimensional (3D) optical imaging of rapidly moving biological cells is difficult to achieve as such samples cannot be scanned over time. Here, we present a dynamic scan-free optical tomography approach for stain-free 3D imaging of biological cells using our new double six-pack tomography technique, whereby 12 off-axis holograms are captured in a single camera exposure without sacrificing resolution or field of view. The proposed system illuminates the sample from 12 angles simultaneously, and 3D refractive index (RI) tomograms are reconstructed from each recorded video frame of the dynamic sample. The technique is verified experimentally by recording flowing silica beads, 3 μm in diameter, with the resulting tomogram RI accuracy being 98.5%. A live swimming sperm cell is also imaged, and dynamic 3D imaging results for both beads and sperm cell are presented. The proposed technique represents a 12-fold increase in dynamic holographic data for tomography.

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Section: Discussionmentioning

confidence: 99%

Dynamic Tomographic Phase Microscopy by Double Six-Pack Holography

2022

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“…Snapshot tomography is another possible approach to multiplexing holograms and accelerating acquisitions [ 193 , 194 , 195 , 196 ]. This method, compared to the six-pack method or double six-pack holography, has the advantage of simplicity, through multiplexing the illumination using a microlens array, which translates the detection camera into many sub-apertures, each delivering an off-axis hologram corresponding to a different illumination angle.…”

Section: Present and Future Trendsmentioning

confidence: 99%

Recent Advances and Current Trends in Transmission Tomographic Diffraction Microscopy

Verrier,

Debailleul,

Haeberlé

2024

Sensors

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Optical microscopy techniques are among the most used methods in biomedical sample characterization. In their more advanced realization, optical microscopes demonstrate resolution down to the nanometric scale. These methods rely on the use of fluorescent sample labeling in order to break the diffraction limit. However, fluorescent molecules’ phototoxicity or photobleaching is not always compatible with the investigated samples. To overcome this limitation, quantitative phase imaging techniques have been proposed. Among these, holographic imaging has demonstrated its ability to image living microscopic samples without staining. However, for a 3D assessment of samples, tomographic acquisitions are needed. Tomographic Diffraction Microscopy (TDM) combines holographic acquisitions with tomographic reconstructions. Relying on a 3D synthetic aperture process, TDM allows for 3D quantitative measurements of the complex refractive index of the investigated sample. Since its initial proposition by Emil Wolf in 1969, the concept of TDM has found a lot of applications and has become one of the hot topics in biomedical imaging. This review focuses on recent achievements in TDM development. Current trends and perspectives of the technique are also discussed.

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“…Kusé t al. 31 introduced a high-throughput ODT scheme by segmenting the image sensor into multiple regions over which projections under various illumination angles can be simultaneously recorded. The method is effective in terms of improving the tomographic imaging throughput for a single cell but may compromise the spatial resolution or the field of view.…”

Section: ■ Introductionmentioning

confidence: 99%

“…This method, however, exhibits nonisotropic spatial resolution and image artifacts due to the significant amount of missing information in the object 3D spectrum that can be obtained with a single measurement. Kuś et al introduced a high-throughput ODT scheme by segmenting the image sensor into multiple regions over which projections under various illumination angles can be simultaneously recorded. The method is effective in terms of improving the tomographic imaging throughput for a single cell but may compromise the spatial resolution or the field of view.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Measurements of Red Blood Cell Indices Using Spectroscopic Differential Phase-Contrast Microscopy

Moon,

Yang,

Song

et al. 2023

Chemical & Biomedical Imaging

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Red blood cell (RBC) indices serve as clinically important parameters for diagnosing various blood-related diseases. Conventional hematology analyzers provide the highly accurate detection of RBC indices but require large blood volumes (>1 mL), and the results are bulk mean values averaged over a large number of RBCs. Moreover, they do not provide quantitative information related to the morphological and chemical alteration of RBCs at the single-cell level. Recently, quantitative phase imaging (QPI) methods have been introduced as viable detection platforms for RBC indices. However, coherent QPI methods are built on complex optical setups and suffer from coherent speckle noise, which limits their detection accuracy and precision. Here, we present spectroscopic differential phase-contrast (sDPC) microscopy as a platform for measuring RBC indices. sDPC is a computational microscope that produces color-dependent phase images with higher spatial resolution and reduced speckle noise compared to coherent QPIs. Using these spectroscopic phase images and computational algorithms, RBC indices can be extracted with high accuracy. We experimentally demonstrate that sDPC enables the high-accuracy measurement of the mean corpuscular hemoglobin concentration, mean corpuscular volume, mean corpuscular hemoglobin, red cell distribution width, hematocrit, hemoglobin concentration, and RBC count with errors smaller than 7% as compared to a clinical hematology analyzer based on flow cytometry (XN-2000; Sysmex, Kobe, Japan). We further validate the clinical utility of the sDPC method by measuring and comparing the RBC indices of the control and anemic groups against those obtained using the clinical hematology analyzer.

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Real-time, multiplexed holographic tomography

Cited by 18 publications

References 42 publications

Dynamic Tomographic Phase Microscopy by Double Six-Pack Holography

Dynamic Tomographic Phase Microscopy by Double Six-Pack Holography

Recent Advances and Current Trends in Transmission Tomographic Diffraction Microscopy

Quantitative Measurements of Red Blood Cell Indices Using Spectroscopic Differential Phase-Contrast Microscopy

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