Real time PCR detection targeting nifA gene of plant growth promoting bacteria Azospirillum brasilense strain FP2 in maize roots

Faleiro, Alexandro Cézar; Pereira, Tomás Pellizzaro; Espindula, Eliandro; Brod, Fábio Cristiano Angonesi; Arisi, Ana Carolina Maisonnave

doi:10.1007/s13199-013-0262-y

Cited by 24 publications

(21 citation statements)

References 35 publications

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“…seropedicae SmR1 and A. brasilense FP2, two effective N 2 -fixing bacteria now considered as model species for studying their beneficial interactions with several species of cereals, notably maize [46,59,60]. Evaluation of bacterial colonization inside and outside of the roots and leaves of the two maize lines by bacterial cell counting 7 to 14 days after inoculation revealed that both strains were able to colonize the two maize lines (Fig 1).…”

Section: Discussionmentioning

confidence: 99%

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Metabolic profiling of two maize (Zea mays L.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense

et al. 2017

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Maize roots can be colonized by free-living atmospheric nitrogen (N2)-fixing bacteria (diazotrophs). However, the agronomic potential of non-symbiotic N2-fixation in such an economically important species as maize, has still not been fully exploited. A preliminary approach to improve our understanding of the mechanisms controlling the establishment of such N2-fixing associations has been developed, using two maize inbred lines exhibiting different physiological characteristics. The bacterial-plant interaction has been characterized by means of a metabolomic approach. Two established model strains of Nif+ diazotrophic bacteria, Herbaspirillum seropedicae and Azospirillum brasilense and their Nif- couterparts defficient in nitrogenase activity, were used to evaluate the impact of the bacterial inoculation and of N2 fixation on the root and leaf metabolic profiles. The two N2-fixing bacteria have been used to inoculate two genetically distant maize lines (FV252 and FV2), already characterized for their contrasting physiological properties. Using a well-controlled gnotobiotic experimental system that allows inoculation of maize plants with the two diazotrophs in a N-free medium, we demonstrated that both maize lines were efficiently colonized by the two bacterial species. We also showed that in the early stages of plant development, both bacterial strains were able to reduce acetylene, suggesting that they contain functional nitrogenase activity and are able to efficiently fix atmospheric N2 (Fix+). The metabolomic approach allowed the identification of metabolites in the two maize lines that were representative of the N2 fixing plant-bacterial interaction, these included mannitol and to a lesser extend trehalose and isocitrate. Whilst other metabolites such as asparagine, although only exhibiting a small increase in maize roots following bacterial infection, were specific for the two Fix+ bacterial strains, in comparison to their Fix- counterparts. Moreover, a number of metabolites exhibited a maize-genotype specific pattern of accumulation, suggesting that the highly diverse maize genetic resources could be further exploited in terms of beneficial plant-bacterial interactions for optimizing maize growth, with reduced N fertilization inputs.

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Section: Discussionmentioning

confidence: 99%

“…These results include the metabolic events that occurred in a well-controlled gnotobiotic experimental system, when the maize plants were inoculated with two different N 2 -fixing bacteria, Herbaspirillum seropedicae SmR1 and Azospirillum brasilense FP2, and their Fix−counterparts, lacking functional Nase [45,46]. …”

Section: Introductionmentioning

confidence: 99%

Metabolic profiling of two maize (Zea mays L.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense

et al. 2017

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“…Soybean seeds were surface-sterilized by washing them three times with autoclaved ultrapure water, followed by soaking them in 70% ethanol for 3 min, and by a solution of 2% sodium hypochlorite and 2.5% Tween 20 for 30 min. Seeds were then washed three times with sterile distilled water by gentle shaking (Faleiro et al, 2013). All solutions and materials used were sterilized at 120 ºC for 30 min.…”

Section: Discussionmentioning

confidence: 99%

The combined analysis as the best strategy for Dual RNA-Seq mapping

et al. 2019

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“…Genomic DNA was isolated from bacterial cultures H. seropedicae strain SmR1 using Wizard ® Genomic DNA Puri cation Kit (PromegaTM, Madison, WI, USA) with modi cations (Faleiro et al, 2013). Total DNA from maize leaves and roots was isolated using DNeasy Plant Mini Kit (Qiagen, Venlo, Netherlands) as described by the manufacturer with modi cations (Pereira et al, 2014).…”

Section: Dna Isolationmentioning

confidence: 99%

Inoculation of Herbaspirillum Seropedicae Increases Biomass in Maize Roots in the Early Stages of Plant Development

Cunha

Pedrolo

Bueno

et al. 2021

Preprint

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Herbaspirillum seropedicae is a plant growth-promoting bacteria isolated from diverse plant species. In this work, the main objective was to investigate the efficiency of H. seropedicae strain SmR1 in colonizing and increasing maize growth in the early stages of development under greenhouse conditions. Inoculation with H. seropedicae resulted in 10.51 and 19.43% in mean of increase of root biomass concerning non-inoculated controls, mainly in the initial stages of plant development, at 21 days after emergence (DAE). Quantification of H. seropedicae in roots and leaves was performed by quantitative PCR.. H. seropedicae was detected only in maize inoculated roots by qPCR, and a slight decrease in DNA copy number g−1 of fresh root weight was observed from 7 to 21 DAE, suggesting that there was initial effective colonization on maize plants. H. seropedicae strain SmR1 efficiently increased maize root biomass exhibiting its potencial to be used as inoculant in agricultures systems.

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Real time PCR detection targeting nifA gene of plant growth promoting bacteria Azospirillum brasilense strain FP2 in maize roots

Cited by 24 publications

References 35 publications

Metabolic profiling of two maize (Zea mays L.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense

Metabolic profiling of two maize (Zea mays L.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense

The combined analysis as the best strategy for Dual RNA-Seq mapping

Inoculation of Herbaspirillum Seropedicae Increases Biomass in Maize Roots in the Early Stages of Plant Development

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