Real-time PCR protocol for the detection of porcine parvovirus in field samples

Wilhelm, Sonja; Zimmermann, Pia; Selbitz, Hans Joachim; Truyen, Uwe

doi:10.1016/j.jviromet.2006.01.004

Cited by 44 publications

(23 citation statements)

References 12 publications

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“…Virus isolation in cell culture is laborious and time consuming and it cannot be achieved for all PPV strains. Other diagnostic techniques, such as conventional PCR, multiplex PCR or real-time PCR are widely used in the diagnosis of porcine parvovirus [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Pseudorabies Virus (PrV), Porcine Parvovirus (PPV) and Porcine Circovirus 2 (PCV2) in Swine in Republic of Montenegro

et al. 2016

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The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identifi ed in this study showed high similarity and signifi cant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and signifi cant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identifi ed in swine in Republic of Montenegro. Besides that, these results confi rmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine.

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Section: Introductionmentioning

confidence: 99%

Molecular Detection of Pseudorabies Virus (PrV), Porcine Parvovirus (PPV) and Porcine Circovirus 2 (PCV2) in Swine in Republic of Montenegro

et al. 2016

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“…Viral genome replication levels were monitored by quantitative PCR (qPCR) of cell lysates harvested at different times postinfection (p.i.) as described previously (4,26). Results are expressed as the increase in the log of viral genome copy equivalents (GCE)/cell over initial GCE/cell at 8 h p.i.…”

mentioning

confidence: 99%

Genetic Elements in the VP Region of Porcine Parvovirus Are Critical to Replication Efficiency in Cell Culture

Fernandes

Boisvert

Tijssen

2011

J Virol

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Factors controlling porcine parvovirus (PPV) replication efficiency are poorly characterized. Two prototype strains of PPV, NADL-2 and Kresse, differ greatly in pathogenic capacity both in vivo and in vitro, yet their genomic sequence is nearly identical (13 single-nucleotide substitutions and a 127-nucleotide noncoding repeated sequence). We have created a series of chimeras of these strains to identify the genetic elements involved in replication efficiency in the host porcine cell line. While the capsid proteins ultimately determine viral replication fitness, interaction between the NS1 protein and the VP gene occurs and involves interaction with the noncoding repeated sequence.

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“…Transplacental infection of fetuses occurs readily and may lead to death and mummification of the fetus. The reproductive disorder in swine is known as stillbirth, mummification, embryonic death and infertility (SMEDI) syndrome (Soares et al, 1999;Zee & McLachlan, 2004;Cao et al, 2005;Wilhelm et al, 2006;Wolf et al, 2008). The establishment of clinical disease and severity of symptoms depend on the virulence of the virus strain and the stage of gestation at the time point of infection.…”

mentioning

confidence: 99%

Vaccination against porcine parvovirus protects against disease, but does not prevent infection and virus shedding after challenge infection with a heterologous virus strain

Jóźwik

Manteufel

Selbitz

et al. 2009

Journal of General Virology

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The demonstration of field isolates of porcine parvovirus (PPV) that differ genetically and antigenically from vaccine strains of PPV raises the question of whether the broadly used inactivated vaccines can still protect sows against the novel viruses. Ten specific-pathogen-free primiparous sows were assigned to three groups and were vaccinated with one of two vaccines based on the old vaccine strains, or served as non-vaccinated controls. After insemination, all sows were challenged with the prototype genotype 2 virus, PPV-27a, on gestation day 41; fetuses were delivered on gestation day 90 and examined for virus infection. The fetuses of the vaccinated sows were protected against disease, but both the vaccinated and the non-vaccinated sows showed a marked increase in antibody titres after challenge infection, indicating replication of the challenge virus. All sows (vaccinated and non-vaccinated) shed the challenge virus for at least 10 days after infection, with no difference in the pattern or duration of virus shedding.

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Real-time PCR protocol for the detection of porcine parvovirus in field samples

Cited by 44 publications

References 12 publications

Molecular Detection of Pseudorabies Virus (PrV), Porcine Parvovirus (PPV) and Porcine Circovirus 2 (PCV2) in Swine in Republic of Montenegro

Molecular Detection of Pseudorabies Virus (PrV), Porcine Parvovirus (PPV) and Porcine Circovirus 2 (PCV2) in Swine in Republic of Montenegro

Genetic Elements in the VP Region of Porcine Parvovirus Are Critical to Replication Efficiency in Cell Culture

Vaccination against porcine parvovirus protects against disease, but does not prevent infection and virus shedding after challenge infection with a heterologous virus strain

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