Real-Time PCR studies regarding the borrelia burgdorferi, francisella tularensis, tick borne encephalitis virus (TBEv) and crimeean congo hemorrhagic fever virus (CCHFv) occurrence in the Romanian ticks

Vladimirescu, A.; Dumitrescu, G.; Ionescu, L; Necşulescu, Marius; Moraru, Valentina; Popescu, D.; Bicheru, Simona; Daneş, Doina; Baraitareanu, D.; Ciulacu-Purcarea, V.; Nicolescu, Gabriela

doi:10.1016/j.ijid.2016.02.448

Cited by 6 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…platys Ab)(Table 4). Despite several Romanian reports concerning the circulation of Ehrlichia canis and B. burgdorferi in dogs(Kiss et al, 2011;Mircean et al, 2012;Morar et al, 2015;Enache & Coprean, 2015) and in tickvectors(Vladimirescu et al, 2016), in our study non Ehrlichia and Borrelia species were detected in…”

contrasting

confidence: 92%

Vector-Borne Pathogens-Testing in a Romanian Canine Blood Bank

Ionescu

Rădulescu

Florea

et al. 2018

CBUP

View full text Add to dashboard Cite

INTRODUCTION: Canine blood banking in veterinary medicine is an expanding market. Once the demand for blood products increased all over the world, canine blood banks have focused attention on the risk of spreading diseases through blood transfused products. The need to preserve a healthy donor-pool, free of blood-borne infectious diseases, mainly in endemic areas, led to the implementation of appropriate protocols for screening canine blood donors using specific tests. OBJECTIVES: The aim of this study was to evaluate the presence of Anaplasma phagocytophilum/Anaplasmaplatys, Echrlichiacanis/Echrlichiaewingii, Dirofilariaimmitis and Borrelia burgdorferi using the enzyme immunoassay technology (EIA) among the donors of a Romanian canine blood bank, from January 2015 to December 2016. METHODS: Blood samples from 575 donors were collected and 1253 tests were performed with SNAP 4DX Plus® (IDEXX Laboratories, Fremont, CA) to reveal the presence of D. immitis antigens and the antibodies toward A. phagocytophilum and/or A. platys, E. canis and/or E. ewingii and B. burgdorferi. RESULTS: The results of this holistic approach show that all blood samples provided negative results for B. burgdorferi and E.canis/E. ewingii (0/1253), while 0.87% (11/1253) samples provided positive results for A. phagocytophilum/A. platys and 6.94% (87/1253) for D. immitis. CONCLUSION: The next studied topic would be to compare the results provided by the EIA technology with results of real time PCR and qPCR regarding these vector-borne pathogens. UDC Classification: 616.9; DOI: http://dx.

show abstract

contrasting

confidence: 92%

Vector-Borne Pathogens-Testing in a Romanian Canine Blood Bank

Ionescu

Rădulescu

Florea

et al. 2018

CBUP

View full text Add to dashboard Cite

show abstract

“…All 14 cases originated in 5 counties in northwestern Romania; incidence was low (<0.5/100,000 inhabitants) in Bihor, Bistrița-Năsăud, Cluj, and Mureș and moderate (0.5–4.0/100,000 inhabitants) in Sălaj. In a recent survey done by collecting I. ricinus ticks from vegetation, livestock, and reptiles in central and southeastern Romania, the prevalence the TBE virus estimated by using molecular methods was <1% ( 13 ). Coipan et al evaluated the seropositivity for TBE in 51 patients who had neurologic signs and were admitted to a hospital in Sibiu County, Romania; specific TBE antibodies were found in samples from 38 (75%) patients ( 14 ).…”

Section: Discussionmentioning

confidence: 99%

Tick-Borne Encephalitis in Sheep, Romania

Salát¹,

Mihalca²,

Mihaiu³

et al. 2017

Emerg. Infect. Dis.

View full text Add to dashboard Cite

show abstract

“…All positive samples were from one site in Sibiu County. Although the overall seroprevalence for Sibiu County was 4.9%, because all the positive samples were from one locality, the seroprevalence was 9.72% CI (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). We did not find any significant difference regarding potential risk factors for TBEV infection (see Table 3).…”

Section: Serum Neutralization Test Resultsmentioning

confidence: 69%

“…Thus, in a study published in 2009, analyzing vector arthropods collected between 1985 and 1993, nine TBEV isolates were obtained from ticks collected from farm animals in five counties (Hunedoara, Tulcea, Mures, Alba, Caras-Severin) [ 4 ]. In another study, in three Romanian counties (Sibiu, Tulcea, Giurgiu) selected as tick sampling sites, specific RNAs from TBEV (3′ UTR- genomic region) were detected in <1% of I. ricinus in the ticks collected from vegetation, livestock and reptiles [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Serosurvey of Tick-Borne Encephalitis Virus Infection in Romania

Panciu,

Cheran,

Militaru

et al. 2024

Pathogens

View full text Add to dashboard Cite

Background: Tick-borne encephalitis (TBE) is a disease with mandatory declaration in the EU since 2012. Information regarding the seroprevalence of the disease across Romania is limited, and only sporadic cases are rarely reported. We aimed to identify new areas of TBEV infection in different counties of Romania. Methods: We conducted a serosurvey assessing the immune response to TBEV infection in adult populations from rural areas in different counties of the country. Seropositivity was defined by a positive TBEV IgM/IgG ELISA test and confirmed by serum neutralization. Results: We collected 1116 samples from 15 different localities in 10 counties (divided into endemic/border/non-endemic counties) across Romania. Overall, 26 (2.3%) of the samples were tested positive using the TBEV ELISA assay in six counties. In those counties, 3.7% of sera were positive, varying from 1.4% to 6.9% per county. After performing the neutralization assay, seven (0.6%) samples were confirmed positive, interestingly all from one site in Sibiu County, where the seroprevalence was 9.7%. Conclusions: The identification of positive serum samples in serosurveys appears to rely on the discovery of TBEV microfoci. Further serological surveys should be conducted in Romania, especially after the identification of positive TBEV patients presenting for medical care.

show abstract

Real-Time PCR studies regarding the borrelia burgdorferi, francisella tularensis, tick borne encephalitis virus (TBEv) and crimeean congo hemorrhagic fever virus (CCHFv) occurrence in the Romanian ticks

Cited by 6 publications

References 0 publications

Vector-Borne Pathogens-Testing in a Romanian Canine Blood Bank

Vector-Borne Pathogens-Testing in a Romanian Canine Blood Bank

Tick-Borne Encephalitis in Sheep, Romania

Serosurvey of Tick-Borne Encephalitis Virus Infection in Romania

Contact Info

Product

Resources

About