Real-Time Polymerase Chain Reaction For Detection Of Optimal Thermal Profile For DNA Templates From Air-Dried Insect Collection Material

Kokina, Inese; Barševskis, A.; Latvele, Rita; Aksjuta, Kristīna; Jahundoviča, Inese

doi:10.1515/prolas-2015-0003

Cited by 2 publications

(3 citation statements)

References 42 publications

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“…A considerable part of IBULC specimens were sampled forty to fifty years ago. These historical arthropod specimens are useful in a wide range of studies, including genetic analysis (Bulat and Zakharov 1992;Harper, Maclean, and Goulson 2006;Kokina et al 2015), population genetics and microevolution (Holmes et al 2016;Wandeler et al 2007), and for investigations using stable isotopes (Hardenbroek et al 2012). Schimmelmann et al (1986) described the study of archaeological chitin samples for environmental and climatic reconstruction research.…”

Section: Historical Specimensmentioning

confidence: 99%

“…Use of the type material is often a top priority and an obligate element during the identification procedures for large taxonomical groups or "problematic" taxa (Enghoff and Seberg 2006;Lindemann 1846;Notton 2007;NSCA 2005;Singh 2007;Singh and Singh 2012;Walker et al 1999). Gutiérrez and Pine (2017) pointed out that according to the International Code of Zoological Nomenclature (ICZN 1999), specimens of presumably new species must first be compared with types of closely related taxa of the same taxonomical group before publishing a new description (Ellis 2008;Kokina et al 2015;Ownes and Duin 2008;Thomson 2005;Wiedenmann, Dowling, and Barnes 2014). In addition, Naumann et al (1994) discussed the importance of secondary types (those that are not holo-or lectotypes).…”

Section: Type Materialsmentioning

confidence: 99%

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The Importance and Potential Value of a Regional Midsize Arthropod Collection: An Example of IBULC

Kagainis¹,

Cera²,

Jucevica³

et al. 2020

The International Journal of the Inclusive Museum

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During the last three centuries, people involved in natural history sciences have produced thousands of natural history collections worldwide. Specimens have been used for scientific proof of various discoveries and for gaining new knowledge in many disciplines of science not only in the area of biodiversity but also in taxonomy, species biology and ecology, parasitology, evolution, organisms' responses to climate change, nature conservation and many other biological sub-disciplines. Despite never-ending financial struggles, the world's largest collections as well as many regional midsize collections contain billions of specimens. This provides an enormous database for present and future studies. This article presents a critical description of the material of the Institute of Biology, University of Latvia collection (IBULC) containing roughly 59,000 identified arthropod individuals of about 3,700 species, including twenty-eight primary types as a part of around ninety type specimens, to illustrate the importance of midsize regional natural history collections in detail.

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Section: Historical Specimensmentioning

confidence: 99%

Section: Type Materialsmentioning

confidence: 99%

The Importance and Potential Value of a Regional Midsize Arthropod Collection: An Example of IBULC

Kagainis¹,

Cera²,

Jucevica³

et al. 2020

The International Journal of the Inclusive Museum

View full text Add to dashboard Cite

show abstract

“…However, lack of knowledge regarding the contribution of each parameter involved in such analysis does not allow for quantitative conclusions. Each step in community analysis is open to error or bias , including cell lysis, DNA extraction and purification , primer selection, PCR conditions , and cloning. Although, many studies have attempted to reduce the PCR bias and artifacts in artificial communities, it remains difficult to apply these results to environmental samples.…”

Section: Introductionmentioning

confidence: 99%

Metagenome complexity and template length are the main causes of bias in PCR‐based bacteria community analysis

Peng

Wang

et al. 2018

J Basic Microbiol

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Multitemplate PCR is used widely for the study of microbial community diversity. Although such studies have established the abundance of different groups within many natural ecosystems, these reports are limited by uncertainties such as bias and artifacts in the PCR. Bias which is introduced by the simultaneous amplification of specific genes from complex mixtures of templates remains poorly understood. In this study, factors leading to the bias of the multitemplate PCR in bacterial communities were examined and optimized. Comparisons between PCR cycle parameters, DNA polymerases, PCR primer degeneracy, and 16S rRNA gene fragments GC content, revealed that annealing temperatures and DNA structure are predominant factors contributing to the observed bias. Pre-digestion of metagenomic DNA with the restriction enzyme Sau3A I and decreased annealing temperature reduced the bias significantly. The application of these optimized conditions to the ten-species model community in a soil sample verified the validity of these treatments.

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Real-Time Polymerase Chain Reaction For Detection Of Optimal Thermal Profile For DNA Templates From Air-Dried Insect Collection Material

Abstract: Contributed by Arvîds BarðevskisThe thermal profile for polymerase chain reaction (PCR)

Cited by 2 publications

References 42 publications

The Importance and Potential Value of a Regional Midsize Arthropod Collection: An Example of IBULC

The Importance and Potential Value of a Regional Midsize Arthropod Collection: An Example of IBULC

Metagenome complexity and template length are the main causes of bias in PCR‐based bacteria community analysis

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