Real‐time quantitative PCR‐based detection of <i>Coxiella burnetii</i> in unpasteurized cow's milk sold for human consumption

Ribeiro, Bruna Letícia Devidé; Peres, Marina Gea; Teixeira, Wanderson Sirley Reis; Pelícia, Vanessa Cristina; Motta, Rodrigo Garcia; Labruna, Marcelo Bahia; Ribeiro, Márcio Garcia; Sidi-Boumedine, Karim; Megid, Jane

doi:10.1111/zph.12609

Cited by 20 publications

(17 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, recent multi spacer typing (MST) and multi-locus variable tandem repeat analysis (MLVA) investigations in Brazil have identified evidence of unique genotypes [ 17 ], and this emphasizes the need of epidemiological and virulence studies that aim to better understand the disease in this region. MST-74, detected in Brazil, belongs to the same phylogenetic branch as MST 20 and MST 61 [ 16 ], which places the strains circulating in Brazil in genomic group III of Hendrix et al [ 70 – 72 ]. In a recent virulence study, it was shown that strains belonging to genomic groups I and III were more virulent, with increased pathogenic potential compared to C .…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, the possibility of transmission through consumption of cattle milk and dairy products should not be neglected, as the presence of C . burnetii has been evaluated in such products in Brazil [ 16 , 76 ].…”

Section: Discussionmentioning

confidence: 99%

“…Later, Riemann et al[ 14 ] reported a seroprevalence of 29% (45/156) in beef cattle. Recently, a survey using a commercial ELISA found 55.1% (172/312) seropositivity in a goat herd with reproductive disorders [ 15 ], and Mioni et al [ 16 ] reported the presence of C . burnetii in dairy cattle and their fetuses in the state of Goiás [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Coxiella burnetii in slaughterhouses in Brazil: A public health concern

et al. 2020

Self Cite

View full text Add to dashboard Cite

Q fever is an important zoonosis, yet it is often neglected and can present large outbreaks, as observed in the Netherlands. In the past few years, cases of Q fever have been described in Brazil; however, the epidemiological situation of Q fever in ruminants, the main reservoir of the pathogen, is unknown in this country. Our study aimed to estimate the prevalence of C . burnetii in cattle sent to slaughterhouses using an immunofluorescence assay (IFA) and quantitative real-time PCR (qPCR). From 1515 cattle serum samples collected from nine slaughterhouses, 23.8% (360/1515) were serologically positive by IFA (cutoff titer>1:64), indicating past or recent exposure to C . burnetii infection. Among the 54 cities sampled during the study, 83.3% (45/54) had at least one seropositive animal. Subsequently, all seropositive samples were submitted to qPCR for C . burnetii DNA, and 12.2% (44/360) of the sera were qPCR positive, which indicates bacteremia and suggests active or recent infection. The results highlight the risk for abattoir workers that results from exposure to contaminated aerosols produced during slaughter procedures. Moreover, the heat maps that were construction from the positive samples demonstrate the widespread distribution of C . burnetii in the State of São Paulo, Brazil and denotes the need for surveillance and preventive measures to reduce the prevalence in cattle.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Coxiella burnetii in slaughterhouses in Brazil: A public health concern

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…We performed qPCR, targeting the IS1111 element with specific primers and probes developed by ANSES (Sophia Antipolis, France) and produced by GENEWIZ (Leipzig, Germany). The quantitative PCR reaction was performed in a total volume of 25 µL, as described by Mioni et al [7]. A standard curve ranging from 2 × 10 2 to 2 × 10 7 genome equivalent (GE) bacteria/mL was used for quantification proposes.…”

Section: Quantitative Pcr (Qpcr)mentioning

confidence: 99%

“…The importance of Q fever, in terms of public health, increased after the outbreak in the Netherlands, where more than 4000 people became ill and 50,000 animals were slaughtered to control the epidemics [5,6]. Recently, we reported the presence of bacteria in Brazil by qPCR in raw Pathogens 2020, 9,30 2 of 11 cow's milk sold directly for human consumption and its risk to human health [7]. The use of molecular typing is useful to determine the source of the outbreak, leading to more precise preventive measures [8].…”

Section: Introductionmentioning

confidence: 99%

New Genotypes of Coxiella burnetii Circulating in Brazil and Argentina

et al. 2019

Self Cite

View full text Add to dashboard Cite

Coxiella burnetii, the zoonotic agent of Q fever, has a worldwide distribution. Despite the vast information about the circulating genotypes in Europe and North America, there is a lack of data regarding C. burnetii strains in South America. Here, we show the presence of novel multispacer sequence typing (MST) genotypes of C. burnetii in two clusters detected in Brazil and Argentina that seem to be distant in parenthood. Argentinian strains isolated from a tick belongs to a new phylogenetic branch of C. burnetii, and the Brazilians strains may be related to MST 20 and 61. Multilocus variable number tandem repeats analysis (MLVA) typing provided a deeper resolution that may be related to host clusters of bovines, caprine, ovine, and ticks. Our results corroborate with the reports of geotypes of C. burnetii. Thus, we highlight the need for more genotyping studies to understand the genetic diversity of C. burnetii in South America and to confirm the hypothesis of host-related genotypes. We also emphasize the importance of virulence studies for a better understanding of Q fever in the region, which may help in surveillance and disease prevention programs.

show abstract

Molecular evidence of sporadic Coxiella burnetii excretion in sheep milk, central Portugal

Pires,

Santos-Silva,

Cruz

et al. 2024

Vet Res Commun

View full text Add to dashboard Cite

Coxiella burnetii is the etiologic agent of Q fever, a worldwide zoonosis. Cattle, sheep and goats are considered the main reservoirs of the disease. Transmission to humans occurs mainly through the inhalation of infectious aerosols from milk, faeces, urine, and birth products from infected ruminants. In this study, a 2-year longitudinal approach was performed to ascertain the excretion of C. burnetii in bulk tank milk samples of sheep from a mountain plateau in central Portugal, with sampling conducted during the years 2015 and 2016. From a total of 156 bulk tank milk samples tested by qPCR, only one showed to be positive for C. burnetii (1.28% [95%CI: 0.03–6.94]), from 2015, the first year of collection. Bidirectional sequencing and phylogenetic analysis of IS1111 transposase partial region confirmed the presence of C. burnetii DNA. The presence of C. burnetii in raw milk samples highlights the necessity for additional research to determine if raw milk is a potential source for human infection. Animal health surveillance and prevention measures against this zoonotic disease should be considered.

show abstract

Real‐time quantitative PCR‐based detection of Coxiella burnetii in unpasteurized cow's milk sold for human consumption

Cited by 20 publications

References 35 publications

Coxiella burnetii in slaughterhouses in Brazil: A public health concern

Coxiella burnetii in slaughterhouses in Brazil: A public health concern

New Genotypes of Coxiella burnetii Circulating in Brazil and Argentina

Molecular evidence of sporadic Coxiella burnetii excretion in sheep milk, central Portugal

Contact Info

Product

Resources

About