Real-Time Reverse Transcription-PCR Expression Profiling of the Complete Human ATP-Binding Cassette Transporter Superfamily in Various Tissues

Abstract

Background: ATP-binding cassette (ABC) transporters are involved in many physiologic processes, such as lipid transport, sterol homeostasis, immune mechanisms, and drug transport, and cause various human inherited diseases. Thus, the analysis of ABC transporter mRNA expression profiles for basic research, especially in the field of lipid metabolism, for clinical diagnosis, and for monitoring of drug effects is of great interest. Methods: We have developed a rapid, accurate, and highly sensitive real-time reverse transcription-PCR (RT-PCR) method for detection and quantification of all 47 currently known members of the ABC transporter superfamily. Our expression analysis is based on relative quantification using a calibration curve method. With our assay, expression monitoring of a large number of RNA samples in a 384-well format with only 50 ng of total RNA is possible. Results: In contrast to previous expression analyses of single ABC genes, our method allows the rapid and complete analysis of all ABC transporters in given RNA samples. We used our newly established expression panel to study the gene expression of all human ABC transporters in 20 different human tissues. As a result, we identified tissues with high transcriptional activity for ABC transporters. These organs are mainly involved

Transcriptional expression analysis of ABC efflux transporters and xenobiotic-metabolizing enzymes in the Chinese rare minnow

“…3). This agrees with previous findings in the rainbow trout (Lončar et al, 2010) and humans (Langmann et al, 2003;Nishimura and Naito, 2005), indicating an important role for P-glycoprotein (ABCB1) in mediating the efflux of xenobiotics into the bile, urine, blood, and intestinal lumen of fish (Lončar et al, 2010). Moreover, mRNA expression from the ABCB1 and ABCB11 genes was not detected in the gill, which agreed with previous findings in the rainbow trout and Nile tilapia (Lončar et al, 2010;Costa et al, 2012).…”

The biological regulation of ABCE1

“…Interestingly, ABCE1 gene has been demonstrated the presence in cytoplasm and mitochondrial, with expression specificity in different tissues. In a real time RT-PCR research on the expressions of the ABC family in different tissues, ABCE1 gene is universally found in tissues, with high expression in testis, prostate, and trachea, low expression in colon, myocardium, and muscle (13). Based on the structure and expression specificity, ABCE1 gene has been linked to a wide biological role in cell physiology.…”

Pregnane-X-receptor mediates the anti-inflammatory activities of rifaximin on detoxification pathways in intestinal epithelial cells

“…The genes involved in drug metabolism provide an adaptive response to environmental challenge, and as a consequence, their expression is tightly regulated by foreign compounds themselves [11]. The ABCB1 (MDR1) and additional ABC transporters with a high expression in the gut such as ABCC1-3 (MRP1-3) are critically involved in the maintenance of the intestinal barrier by excluding drugs, nutrients, or bacterial compounds back into the gut lumen [22,[30][31][32]. Furthermore, the association of MDR1 gene polymorphisms with susceptibility for IBD [23,24] implies that genetic [16,19].…”