Realistic aspects behind the application of the rat model of chemically-induced mammary cancer: Practical guidelines to obtain the best results

Abstract

Cancer is one of the most important public health problems worldwide. Despite the great contribution of in-vitro studies for biomedical research, animals are essential to study diseases’ biopathology and diagnosis, and searching for new preventive and therapeutic strategies. Breast cancer is currently the most common cancer globally, accounting for 12.5% of all new annual cancer cases worldwide. Although the rat model of mammary cancer chemically-induced is widely used to study this disease, there is a lack of standardization in procedures for cancer induction, sample collection, and analysis. Therefore, it is important to provide a practical guide for researchers aiming to work with this model to make the analysis of results more uniform. Thus, in this review, we provide the researchers with a detailed step-by-step guide to implement a rat model of mammary cancer, based on our wide experience in this field, to obtain the best results, maximum throughput of each experiment, and easy comparison among researches. Keywords: carcinogenesis, guide, modeling, rodents, tips.

Contribution of non-steroidal anti-inflammatory drugs to breast cancer treatment: In vitro and in vivo studies

“…Published studies indicate that MNU generates more aggressive mammary tumors than DMBA in a shorter time [ 130 ]. Sprague-Dawley and Wistar rats are the most commonly used strains in mammary carcinogenesis because they are more susceptible to carcinogenic agents than other strains [ 131 ]. A broad spectrum of hormone-positive histological lesions has been observed in rat models of chemically induced mammary tumors [ 132 ].…”