Recellularization on Acellular Lung Tissue Scaffold Using Perfusion-Based Bioreactor: An Online Monitoring Strategy

Doryab, Ali; Heydarian, Motaharehsadat; Amoabediny, Ghassem; Sadroddiny, Esmaeil; Mahfouzi, Seyed Hossein

doi:10.1007/s40846-016-0205-1

Cited by 13 publications

(8 citation statements)

References 24 publications

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“…On the other hand, the advantages of cell and tissue‐based bioreactor systems includes the creation of biomimetic culture conditions that significantly improve the physiological relevance of cell and tissue cultures by controlling microenvironment parameters and facilitating mass transfer of nutrients . Nonetheless, one of the main limitations of the currently available stretchable systems is the lack of suitable membranes, which are both suitable for ALI cell culture conditions and closely mimicking the physiologic conditions in the lung.…”

Section: Introductionmentioning

confidence: 99%

Evolution of Bioengineered Lung Models: Recent Advances and Challenges in Tissue Mimicry for Studying the Role of Mechanical Forces in Cell Biology

Doryab

Taş

Taskin

et al. 2019

Adv Funct Materials

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Mechanical stretch under both physiological (breathing) and pathophysiological (ventilator-induced) conditions is known to significantly impact all cellular compartments in the lung, thereby playing a pivotal role in lung growth, regeneration and disease development. In order to evaluate the impact of mechanical forces on the cellular level, in vitro models using lung cells on stretchable membranes have been developed. Only recently have some of these cell-stretching devices become suitable for air-liquid interface cell cultures, which is required to adequately model physiological conditions for the alveolar epithelium. To reach this goal, a multi-functional membrane for cell growth balancing biophysical and mechanical properties is critical to mimic (patho)physiological conditions. In this review, i) the relevance of cyclic mechanical forces in lung biology is elucidated, ii) the physiological range for the key parameters of tissue stretch in the lung is described, and iii) the currently available in vitro cell-stretching devices are discussed. After assessing various polymers, it is concluded that natural-synthetic copolymers are promising candidates for suitable stretchable membranes used in cell-stretching models. This work provides guidance on future developments in biomimetic in vitro models of the lung with the potential to function as a template for other organ models (e.g., skin, vessels).

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Section: Introductionmentioning

confidence: 99%

Evolution of Bioengineered Lung Models: Recent Advances and Challenges in Tissue Mimicry for Studying the Role of Mechanical Forces in Cell Biology

Doryab

Taş

Taskin

et al. 2019

Adv Funct Materials

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“…Therefore, many researchers aim at recellularising the bare matrices prior to implantation. 80,81 The seeded cells will re-populate the scaffold and deposit new ECM molecules throughout the matrix, thereby preserving its structure and function. 12 Furthermore, seeding the scaffolds with the patient's own cells will create an autologous construct, offering a potentially immunologically inert therapy.…”

Section: Bare Versus Recellularised Matricesmentioning

confidence: 99%

“…Numerous examples can be found in the literature regarding the use of various bioreactor systems for the recellularisation of decellularised scaffolds. 81,[88][89][90] A recent example is the work by Talò et al 90 who used an oscillating stretch-perfusion bioreactor that combined bidirectional perfusion with programmable, uniaxial strain to functionalise cell-seeded decellularised tendons. Decellularised tendon matrices from adult horses were seeded on their surfaces and within the tendon fibres with rabbit bone marrow mesenchymal stem cells and cultured in the bioreactor system for 7 days.…”

Section: Bioreactorsmentioning

confidence: 99%

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Decellularised scaffolds: just a framework? Current knowledge and future directions

et al. 2020

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The use of decellularised matrices as scaffolds offers the advantage of great similarity with the tissue to be replaced. Moreover, decellularised tissues and organs can be repopulated with the patient’s own cells to produce bespoke therapies. Great progress has been made in research and development of decellularised scaffolds, and more recently, these materials are being used in exciting new areas like hydrogels and bioinks. However, much effort is still needed towards preserving the original extracellular matrix composition, especially its minor components, assessing its functionality and scaling up for large tissues and organs. Emphasis should also be placed on developing new decellularisation methods and establishing minimal criteria for assessing the success of the decellularisation process. The aim of this review is to critically review the existing literature on decellularised scaffolds, especially on the preparation of these matrices, and point out areas for improvement, finishing with alternative uses of decellularised scaffolds other than tissue and organ reconstruction. Such uses include three-dimensional ex vivo platforms for idiopathic diseases and cancer modelling.

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“…This system provided a means for noninvasive, real‐time, and repetitive investigation of tissue growth (Mahfouzi et al, 2018; Figure 4). Also, a perfusion‐based bioreactor system (based on measuring the levels of dissolved oxygen in the perfusate at different time points using a two‐sensor setup) was used to monitor the growth behavior of HUVECs during recellularization of acellular rat lung scaffolds (Doryab et al, 2017).…”

Section: Bioreactors For Monitoring Growth and Maturation Of Bioengineered Lungsmentioning

confidence: 99%

Advances in bioreactors for lung bioengineering: From scalable cell culture to tissue growth monitoring

Mahfouzi

Amoabediny

Tali

2021

Biotech & Bioengineering

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Recellularization on Acellular Lung Tissue Scaffold Using Perfusion-Based Bioreactor: An Online Monitoring Strategy

Cited by 13 publications

References 24 publications

Evolution of Bioengineered Lung Models: Recent Advances and Challenges in Tissue Mimicry for Studying the Role of Mechanical Forces in Cell Biology

Evolution of Bioengineered Lung Models: Recent Advances and Challenges in Tissue Mimicry for Studying the Role of Mechanical Forces in Cell Biology

Decellularised scaffolds: just a framework? Current knowledge and future directions

Advances in bioreactors for lung bioengineering: From scalable cell culture to tissue growth monitoring

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