Recent Advances, Challenges, and Opportunities in Riboswitches

Kesharwani, Manish; Velmurugan, D.

doi:10.1007/978-981-15-0081-7_11

Cited by 1 publication

(1 citation statement)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another recently pursued technique is applying riboswitch, a stem-loop RNA structure with regulatory and ligand-binding domains, to modulate mRNA stability and translation in plants [ 426 ]. A synthetic riboswitch library with theophylline acting as a ligand has been created for Arabidopsis to regulate endogenous and transgenes via posttranscriptional regulation [ 427 ].…”

Section: Synthetic Biology For Improving or Redesigning Abiotic Stres...mentioning

confidence: 99%

Biological Parts for Engineering Abiotic Stress Tolerance in Plants

2022

View full text Add to dashboard Cite

It is vital to ramp up crop production dramatically by 2050 due to the increasing global population and demand for food. However, with the climate change projections showing that droughts and heatwaves becoming common in much of the globe, there is a severe threat of a sharp decline in crop yields. Thus, developing crop varieties with inbuilt genetic tolerance to environmental stresses is urgently needed. Selective breeding based on genetic diversity is not keeping up with the growing demand for food and feed. However, the emergence of contemporary plant genetic engineering, genome-editing, and synthetic biology offer precise tools for developing crops that can sustain productivity under stress conditions. Here, we summarize the systems biology-level understanding of regulatory pathways involved in perception, signalling, and protective processes activated in response to unfavourable environmental conditions. The potential role of noncoding RNAs in the regulation of abiotic stress responses has also been highlighted. Further, examples of imparting abiotic stress tolerance by genetic engineering are discussed. Additionally, we provide perspectives on the rational design of abiotic stress tolerance through synthetic biology and list various bioparts that can be used to design synthetic gene circuits whose stress-protective functions can be switched on/off in response to environmental cues.

show abstract

Section: Synthetic Biology For Improving or Redesigning Abiotic Stres...mentioning

confidence: 99%