Recent advances in aptamer-based sensors for aminoglycoside antibiotics detection and their applications

Yue, Fengling; Li, Falan; Kong, Qianqian; Guo, Yemin

doi:10.1016/j.scitotenv.2020.143129

Cited by 66 publications

(28 citation statements)

References 143 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sequences in groups A and B were almost identical, suggesting that the selected RNA was enriched (Figure 6). Our data are consistent with those published previously [19,20,22] where they reported that some of the identified sequences were more or less indistinguishable, indicates the enrichment of the selected RNA. Amikacin is the most effective AG used to treat severe bacterial infections [4].…”

Section: Groupsupporting

confidence: 93%

“…Since in vitro selection was introduced in the early 1990s [19], the power of this combinatorial approach has been used to generate aptamers that bind targets ranging from organic molecules to proteins and even DNA [32][33][34]. It is reported that more than 2000 aptamers have been synthesized during the past 30 years [22]. Accumulating evidence suggests that a large number of generated aptamers can bind various target molecules, including AGs [26][27][28][29].…”

Section: Sequence Analysis Of Amikacin-specific Aptamersmentioning

confidence: 99%

“…In vitro selection of RNAs that bind to target molecules is usually performed over iterative cycles, in a process known as systemic evolution of ligands by exponential enrichment (SELEX) [19,20]. This method has enabled the identification of unique high affinity RNAs (aptamers) that bind to ligands of interest within large random RNA libraries [21,22]. Aptamers are single-stranded short oligonucleotide or peptide molecules that bind to a specific target, including proteins, carbohydrates, and small molecules with high affinity and specificity [23,24].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Screening of Aptamers That Bind to the Multivalent Aminoglycoside Amikacin

Alam¹,

Miura²,

Tsukahara³

2021

Preprint

View full text Add to dashboard Cite

Increased awareness of the multiple roles of RNA molecules has led to the realization that, in addition to their structural and functional roles, RNAs can be drug targets for small molecular therapy. The aim of this study was to identify multivalent amikacin specific RNA aptamers that can be a new target sites for aminoglycoside antibiotics, including amikacin using the systemic evolution of ligands by exponential enrichment (SELEX) method. Amikacin, a member of the aminoglycoside group of antibiotics, binds to specific sites in bacterial 16S ribosomal RNAs (rRNAs) and interferes with protein synthesis, leading to cell death. Here, we used the SELEX method to isolate high affinity RNA fragments (aptamers) that bind to amikacin. After five rounds of SELEX selection, in which a linear N25 DNA template was used for the first selection cycle, the resulting RNA was cloned and sequenced. Among the 38 clones generated, five groups of sequences (groups A through E) containing nine conserved motifs were identified. The sequences of groups A and B were almost identical, indicating that the selected RNA was enriched. Subsequently, the Basic Local Alignment Search Tool program was used to search for the conserved motifs in bacterial 16S rRNA sequences. Strikingly, no sequence homology was observed, suggesting that the conserved sequences (motifs) identified in this study may be novel target sites for amikacin.

show abstract

Section: Groupsupporting

confidence: 93%

Section: Sequence Analysis Of Amikacin-specific Aptamersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Screening of Aptamers That Bind to the Multivalent Aminoglycoside Amikacin

Alam¹,

Miura²,

Tsukahara³

2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Colorimetric biosensors based on aptamers have demonstrated their sensitivity and selectivity, in addition to their effective potential for rapid onsite diagnosis without complicated instrumentation. However, the colorimetric aptasensor has some limitations such as the influence of color from samples; the time-consuming nature of the fabrication process [57,58]; the difficulty in employing it in multiple-target assays, which are highly demanded in clinical diagnosis [59]; and the small range of optimized pH solutions [60].…”

Section: Colorimetric Aptasensormentioning

confidence: 99%

Recent Advances in Aptamer Sensors

Shaban

Kim

2021

Sensors

View full text Add to dashboard Cite

Recently, aptamers have attracted attention in the biosensing field as signal recognition elements because of their high binding affinity toward specific targets such as proteins, cells, small molecules, and even metal ions, antibodies for which are difficult to obtain. Aptamers are single oligonucleotides generated by in vitro selection mechanisms via the systematic evolution of ligand exponential enrichment (SELEX) process. In addition to their high binding affinity, aptamers can be easily functionalized and engineered, providing several signaling modes such as colorimetric, fluorometric, and electrochemical, in what are known as aptasensors. In this review, recent advances in aptasensors as powerful biosensor probes that could be used in different fields, including environmental monitoring, clinical diagnosis, and drug monitoring, are described. Advances in aptamer-based colorimetric, fluorometric, and electrochemical aptasensing with their advantages and disadvantages are summarized and critically discussed. Additionally, future prospects are pointed out to facilitate the development of aptasensor technology for different targets.

show abstract

“…By sensing different kinds of extracellular AMG residues, we identified an indirect method for microbial detection and discrimination. Over the past decades, a series of methods have been developed to detect AMG residues [26,27]. Colorimetric sensors based on noble metallic nanoparticles (Au and AgNPs) have been exploited in AMG detection because of their high sensitivity [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Array-based microbial identification upon extracellular aminoglycoside residue sensing

Liu

et al. 2021

Anal Bioanal Chem

View full text Add to dashboard Cite

Sensitive and rapid identification of pathogenic microorganisms is of great importance for clinical diagnosis and treatment. In this study, we developed an ultrasensitive colorimetric sensor array (CSA) based on the interactions between aminoglycoside antibiotics (AMGs) and Ag nanoparticles decorated with β-cyclodextrin (AgNPs@β-CD) to discriminate microorganisms quickly and accurately. Microorganisms can absorb different amounts of AMGs after incubation. Upon the addition of AgNPs@β-CD, the corresponding extracellular AMG residues will bind to AgNPs@β-CD, leading to color changes due to the modifications in localized surface plasmon resonance. The array was developed using 4 AMGs as sensing elements and AgNPs@β-CD as the colorimetric probe to generate a unique colorimetric response pattern for each microorganism. Standard chemometric methods indicated excellent discrimination among 20 microorganisms at low concentrations of 2 × 10 6 CFU/mL. Therefore, this ultrasensitive CSA can be used for microbial discrimination portably and efficiently. Importantly, the concentration of microbial discrimination by our array is much lower than that of prior CSAs. This method of extracellular residue sensing also provided a new strategy to improve the sensitivity of conventional CSA in the discrimination of microorganisms, to measure the amount of intercellular uptake of AMGs by microorganisms, and to screen drugs that can easily be accumulated by the pathogenic microorganisms. Keywords UV/VIS . Optical sensors . Nanoparticles . Bioanalytical methods . Biomedical analysis Published in the topical collection Analytical Chemistry for Infectious Disease Detection and Prevention with guest editors Chaoyong Yang and XiuJun (James) Li.

show abstract

Recent advances in aptamer-based sensors for aminoglycoside antibiotics detection and their applications

Cited by 66 publications

References 143 publications

Screening of Aptamers That Bind to the Multivalent Aminoglycoside Amikacin

Screening of Aptamers That Bind to the Multivalent Aminoglycoside Amikacin

Recent Advances in Aptamer Sensors

Array-based microbial identification upon extracellular aminoglycoside residue sensing

Contact Info

Product

Resources

About