Recent Advances in Aptasensor for Cytokine Detection: A Review

Kim, Jinmyeong; Noh, Samuel; Park, Jeong Ah; Park, Sang C.; Park, Seong Jun; Lee, Jinho; Ahn, Jae-Hyuk; Lee, Taek

doi:10.3390/s21248491

Cited by 23 publications

(18 citation statements)

References 128 publications

(148 reference statements)

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“…A fluorescence-based biosensor is based on the basic principle of visualizing the fluorescence signal that appears in a fluorescent dye, and the presence of an analyte is confirmed by a change in the intensity of the fluorescence signal [ 119 ]. Fluorescence-based biosensors have many advantages, such as good selectivity, high sensitivity, multiplex analysis, simple instrumentation, fast analysis time, and simple operation.…”

Section: Optical-based Detectionmentioning

confidence: 99%

Recent Developments in DNA-Nanotechnology-Powered Biosensors for Zika/Dengue Virus Molecular Diagnostics

Park

et al. 2023

Nanomaterials

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Zika virus (ZIKV) and dengue virus (DENV) are highly contagious and lethal mosquito-borne viruses. Global warming is steadily increasing the probability of ZIKV and DENV infection, and accurate diagnosis is required to control viral infections worldwide. Recently, research on biosensors for the accurate diagnosis of ZIKV and DENV has been actively conducted. Moreover, biosensor research using DNA nanotechnology is also increasing, and has many advantages compared to the existing diagnostic methods, such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). As a bioreceptor, DNA can easily introduce a functional group at the 5′ or 3′ end, and can also be used as a folded structure, such as a DNA aptamer and DNAzyme. Instead of using ZIKV and DENV antibodies, a bioreceptor that specifically binds to viral proteins or nucleic acids has been fabricated and introduced using DNA nanotechnology. Technologies for detecting ZIKV and DENV can be broadly divided into electrochemical, electrical, and optical. In this review, advances in DNA-nanotechnology-based ZIKV and DENV detection biosensors are discussed.

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Section: Optical-based Detectionmentioning

confidence: 99%

Recent Developments in DNA-Nanotechnology-Powered Biosensors for Zika/Dengue Virus Molecular Diagnostics

Park

et al. 2023

Nanomaterials

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“…The cell-released proteins, such as cytokines and proteases, play critical roles in regulating physiology and homeostasis in the change process of the extracellular environment. − Optical spectroscopies based on fluorescence, SERS, and SPR have been exploited in the protein secretion analysis (Table ). A fluorescent immunoassay-based single cell analysis platform has been developed for investigating the differential modulation effect in cytokine secretions by the tumor microenvironment (Figure A) .…”

Section: Approaches To Single Cellmentioning

confidence: 99%

Optical Sensing Strategies for Probing Single-Cell Secretion

et al. 2022

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Measuring cell secretion events is crucial to understand the fundamental cell biology that underlies cell–cell communication, migration, proliferation, and differentiation. Although strategies targeting cell populations have provided significant information about live cell secretion, they yield ensemble profiles that obscure intrinsic cell-to-cell variations. Innovation in single-cell analysis has made breakthroughs allowing accurate sensing of a wide variety of secretions and their release dynamics with high spatiotemporal resolution. This perspective focuses on the power of single-cell protocols to revolutionize cell-secretion analysis by allowing real-time and real-space measurements on single live cell resolution. We begin by discussing recent progress on single-cell bioanalytical techniques, specifically optical sensing strategies such as fluorescence-, surface plasmon resonance-, and surface-enhanced Raman scattering-based strategies, capable of in situ real-time monitoring of single-cell released ions, metabolites, proteins, and vesicles. Single-cell sensing platforms which allow for high-throughput high-resolution analysis with enough accuracy are highlighted. Furthermore, we discuss remaining challenges that should be addressed to get a more comprehensive understanding of secretion biology. Finally, future opportunities and potential breakthroughs in secretome analysis that will arise as a result of further development of single-cell sensing approaches are discussed.

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“…Furthermore, unlike antibodies, aptamers can be probed against nonimmunogenic and toxic targets that may be of significance in different infectious disease pathogenesis (Hong et al, 2012). Due to these distinct and favorable advantages of aptamers, recently, the aptamer-based sensors have gained traction in the field of biosensing, especially in cytokine detection (Kim et al, 2021). These aptasensors have also been designed for clinical diagnostics, where they have been utilized in the detection of cancer biomarkers, cancer cells as well as infectious microorganisms (bacteria, virus and prion) (Hong et al, 2012).…”

Section: Trends In Biosensor Design and Scheme Of Detectionmentioning

confidence: 99%

Sensing Soluble Immune Checkpoint Molecules and Disease-Relevant Cytokines in Cancer: A Novel Paradigm in Disease Diagnosis and Monitoring

et al. 2022

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Conventional detection of cancer involves highly invasive and expensive diagnostic procedures, often leading to non-compliance from patients. Therefore, there is a strong requirement for the development of non-invasive techniques that can facilitate rapid and timely diagnosis of the disease. The tumor-immune interaction often leads to anomalous expression of different soluble immune signaling molecules like cytokines and chemokines, thus making them promising candidates for sensing disease development and progression. Furthermore, differential expression of soluble isoforms of several immune-checkpoint molecules like PD-L1, CTLA-4 etc., has been found to have strong correlation with tissue-specific tumor development, disease progression and in many cases, disease prognosis. Therefore, development of biosensors, to rapidly detect and analyze the levels of these soluble immune molecules in different body fluids, requiring minimal sample volume, has the potential to be a game-changer in the field of cancer diagnosis. In addition, real time monitoring of these soluble immune checkpoint molecules in patient-derived biofluids may serve as decision support tools for patient selection for immunotherapeutic interventions. Majority of the biosensors designed to detect the soluble immune biomarkers, have used a two-antibody based sandwich system to capture the target analyte. However, new technologies using bioreceptors like the aptamers or nano-yeast scFv antibody fragments have made possible multiplexed detection of several analytes simultaneously. The use of gold nanoparticles or carbon nanotubes on the electrode surface serves to increase the sensitivity of detection, due to their high electrical conductivity. Further, fabrication of the biosensors on microfluidic platforms enable the detection of these analytes at ultra-low levels. This review discusses the recent advances made in the development of biosensors for specific and selective detection of these immune-markers that can be successfully translated to the clinics as a new paradigm in disease diagnosis and monitoring.

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Recent Advances in Aptasensor for Cytokine Detection: A Review

Cited by 23 publications

References 128 publications

Recent Developments in DNA-Nanotechnology-Powered Biosensors for Zika/Dengue Virus Molecular Diagnostics

Recent Developments in DNA-Nanotechnology-Powered Biosensors for Zika/Dengue Virus Molecular Diagnostics

Optical Sensing Strategies for Probing Single-Cell Secretion

Sensing Soluble Immune Checkpoint Molecules and Disease-Relevant Cytokines in Cancer: A Novel Paradigm in Disease Diagnosis and Monitoring

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